專利名稱：人類肝臟相關(guān)基因的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物技術(shù)領(lǐng)域，尤其涉及一類在人類肝臟中表達(dá)的相關(guān)基因。
背景技術(shù)：
肝臟是人體的第一大器官，是物質(zhì)代謝、能量轉(zhuǎn)換及供應(yīng)的“樞紐”，是機(jī)體內(nèi)多種重要信息調(diào)控分子的“集散地”，是機(jī)體內(nèi)再生能力最強(qiáng)的器官，在人類生命活動(dòng)中占有重要地位。肝臟的主要功能是進(jìn)行糖的分解、貯存糖原；參與蛋白質(zhì)、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機(jī)能；制造凝血因子；調(diào)節(jié)血容量及水電解質(zhì)平衡；產(chǎn)生熱量等。在胚胎時(shí)期肝臟還有造血功能。
肝臟是淋巴細(xì)胞以外最常見(jiàn)的病原體持續(xù)感染的場(chǎng)所，肝臟疾病又分為肝炎、肝硬化、脂肪肝、肝癌等?，F(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)證明，肝病病毒侵入人體后，并不直接引起肝細(xì)胞的損害，只是在肝細(xì)胞內(nèi)吸收營(yíng)養(yǎng)賴以生存，并在肝細(xì)胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細(xì)胞膜上，引起人體免疫系統(tǒng)對(duì)這些抗原物質(zhì)產(chǎn)生免疫反應(yīng)，這種反應(yīng)造成肝細(xì)胞的損傷、壞死。免疫反應(yīng)的強(qiáng)弱決定于肝臟受損程度及臨床癥狀輕重。這場(chǎng)由病毒引發(fā)的、免疫系統(tǒng)對(duì)肝細(xì)胞的戰(zhàn)爭(zhēng)，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場(chǎng)，肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身，它還可以引起其它多種疾病。常見(jiàn)的有(1)糖尿??；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎??；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結(jié)節(jié)性動(dòng)脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對(duì)患者的身體甚至生命造成危害，而且對(duì)患者心理上的打擊也是十分沉重的。無(wú)論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學(xué)等方面都會(huì)受到嚴(yán)重影響。
肝臟疾病相關(guān)基因在以肝炎、肝癌為代表的重大肝病的控制、診斷、防治與新藥研制領(lǐng)域起著重要的作用。
在本發(fā)明之前，還沒(méi)有出現(xiàn)涉及本發(fā)明的一類人類肝臟相關(guān)基因的公開(kāi)報(bào)道。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問(wèn)題是提供一類在人類肝臟中表達(dá)的相關(guān)基因。
為了解決上述技術(shù)問(wèn)題，本發(fā)明通過(guò)如下技術(shù)方案實(shí)現(xiàn)在本發(fā)明的一個(gè)方面，提供了一類在人類肝臟中表達(dá)的相關(guān)基因，其包括(a)SEQ ID No.1～SEQ ID No.47所示的序列；(b)SEQ ID No.1～SEQ ID No.47所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.47所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.47所示的序列。
本發(fā)明還提供了一種探針?lè)肿樱龅奶结樂(lè)肿雍猩鲜鲂蛄兄屑s8-100個(gè)連續(xù)的核苷酸。
由本發(fā)明的一類在人類肝臟中表達(dá)的相關(guān)基因，可為研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
具體實(shí)施例方式
下面結(jié)合具體實(shí)施例，進(jìn)一步闡述本發(fā)明。應(yīng)理解，這些實(shí)施例僅用于說(shuō)明本發(fā)明而不是限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來(lái)源于5個(gè)成年男性，在肝臟切除手術(shù)后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內(nèi)，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實(shí)施例2cDNA文庫(kù)的構(gòu)建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補(bǔ)平末端后，加含EcoRI切點(diǎn)的接頭。磷酸化EcoRI末端后，用XhoI限制性內(nèi)切酶消化1.5小時(shí)，再進(jìn)行片斷分離。過(guò)柱篩選長(zhǎng)度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無(wú)菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進(jìn)行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細(xì)菌。涂板并測(cè)定滴度。
實(shí)施例3測(cè)序及數(shù)據(jù)庫(kù)建立(Seqencing and Database Constructing)挑選文庫(kù)中有外源片段插入的克隆，擴(kuò)增后抽提質(zhì)粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標(biāo)記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測(cè)序儀(Perkin-Elmer，USA)上進(jìn)行EST大規(guī)模測(cè)序。測(cè)序結(jié)果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450 Server上進(jìn)行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(kù)(Genebank+EMBL)，將無(wú)同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫(kù)。
實(shí)施例4基因的全長(zhǎng)克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上，進(jìn)行cDNA全長(zhǎng)克隆，分兩階段進(jìn)行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針?biāo)褜bEST數(shù)據(jù)庫(kù)，將重疊序列＞50bp，同源性在98％以上的表達(dá)序列標(biāo)簽(Expressed Sequence Tag，簡(jiǎn)稱“EST”)序列認(rèn)為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進(jìn)行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開(kāi)放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長(zhǎng)完整性如何。通過(guò)電子克隆的方法，通?？色@取人肝臟相關(guān)基因的全長(zhǎng)序列。
(2)cDNA末端快速擴(kuò)增(Rapid Amplification of cDNA Ends，RACE)如果通過(guò)“電子克隆”方法仍未得到完整的cDNA全長(zhǎng)，則在已有序列5’或3’端設(shè)計(jì)引物，在人類肝臟Marathon-Ready cDNA文庫(kù)(Clontech Lab，Inc，USA)中進(jìn)行長(zhǎng)距離PCR反應(yīng)。然后對(duì)PCR產(chǎn)物克隆、測(cè)序。用AUTOASSEMBLER及STRIDER軟件分析被延長(zhǎng)的序列有無(wú)完整的ORF，如無(wú)，重復(fù)上述過(guò)程直至獲得全長(zhǎng)。
(3)RT-PCR對(duì)于5’和3’端的已知的序列，如果中間有一段間隙(gap)無(wú)法從已有的公共數(shù)據(jù)庫(kù)或自身數(shù)據(jù)庫(kù)獲得，可考慮采用RT-PCR的方法。在序列5’端設(shè)計(jì)引物，3’端引物采用Oligo-dT，在肝臟總RNA庫(kù)中進(jìn)行擴(kuò)增。然后對(duì)產(chǎn)物進(jìn)行克隆、測(cè)序。最后拼接便獲得全長(zhǎng)。
通過(guò)組合使用上述3種方法，可獲得人肝臟相關(guān)蛋白的全長(zhǎng)編碼序列。
序列表<110>上海人類基因組研究中心<120>人類肝臟相關(guān)基因<130>NP-10161<160>47<210>1<211>498<212>DNA<213>Homo sapiens<400>11 tttgagttat aagtgttgta atagggggta gtaacaagac gttgtaggac tacacaaaat61 aaaacaagga ggccaaatcg caagcgtttg tgggtttcac aggggacatc tgacagcaaa121 cattccaggg caccgagaga tggtgaggag agatgatggc attggagaga tgagctgaga181 tggactgcgc agaggacgag ggaaagccgg tgcactggag acggaaggaa cgcgaggagc241 gcctcatcac caggcgggtg tatctagggg cgaaggctgc agatctgggg cgcaggctgg301 aaagtggggt gcagccggtt gacgtgaggt ctgggagttg gatggaccga atgaggatgg361 agacacctag ggatgtgtcc gaaggatctg gcgctgcaca aatgagggcg gtttggggga421 cggaggcccg ggcgggggcg gagtgttgtc caagagtaag gagggggttc ctggagacgt481 ggcactcgga gcggggct<210>2<211>676<212>DNA<213>Homo sapiens<400>21 gcgtttaaca tttttatttt taactccgct ttggtagtac aaaagtcata aaagtacaaa61 ccagacagtt aaaaatacac ttgacactcg aaatggtgaa aattttcctt acaaattttt121 acatcaaggt agtagccaac tcattgatga caccaaaaag ttgtccatca ttagtgtttt181 ctagagaaag tctgttgtgg attccctcat ccttagaaag gaggaggaga aacacaagac241 ctgtaaacat cagttgcttt gggaacacaa gaattctcat cagatagttc agtataaacc301 agtaaaaagc gtatgtgttg aaaatactga acgcttaatt ttggcaaatt tggaagcctg361 ccagacaaaa accgctcaag tatttattag aaaatattta aaacatactc ttggtatcaa421 tacagtttta aatatttttg agtattctct tgcctgttgt attgctattt aaaaaaaagt481 gctctgactt gaataagatg gaaaaataat taaagctaaa gaatatctta cattttatcc541 cncaccattn tgagggcata ttnttaaagc aaaaaagtat gctttattgt tttaattaaa601 tgatagccta gactgacata tatatntaca ctatacatac ccctaaagtc ctatattgct
661 acttctggct cgtgcc<210>3<211>469<212>DNA<213>Homo sapiens<400>31 cagagcattt aaaggttttt attataatga attctgcaat gaacatcttt ctatataaat61 ttttgtgagt actctgcatt atttcctaaa ataaattaga agaaggaatg atggagaatg121 taaacacttg gagcttttga aacagacagt ttttataaat tagagtctgt agttttaatt181 cagggagatt gttgtgtctc agaagatatt tcacggtgaa gtctcacctg acggcacttc241 ctcgtcctcc gcagagccgt gtgtgtaggg cccagggtgc ggaacgggca gttcttcaac301 acagtgagcg gcagcgggcg tcccgaaggt tctcaggcct tgtctccgtg gagggcattt361 tgtggcctct cccagggcag ccggcaggag ccaggcgaga acagatgcgt ctgtagcagg421 agggcgttga tgcctctgag gttgtagcga ggttggtatt taccttttc<210>4<211>435<212>DNA<213>Homo sapiens<400>41 ttttccaaca atttatccca tgtaatgaaa ttgctaagcc tatatattta tagacacata61 catacacata tatatataca cacacttaca aatatgtatt atatatacag atctatgtat121 aaacactgac atagagggag tgtatagctt ttaattgggc cgtcttctca aaataattaa181 gccagtttta atgaaatact gtttaaggct ggtggcaagc aaagaagcca tctttagcag241 agcattaata aactgagtca ttcgcacaag ggaatggcta gctgtatcag tcacattcat301 ggtgctaata aaagagctca gttctctgtg gcaggccaag gattggaggg ccgggaggga361 gcacagagcc cagtcatctc gggctcactg tcacagacag tccttacggc tgcagtggag421 ttcacagtcc gagct<210>5<211>546<212>DNA<213>Homo sapiens<400>51 tgagtactga atgtctttat tgataaacta aatatatatt taaagcataa tacgataatt61 atgtagagga taaaatataa attgttcact taggagatta atacataaat catatttaca121 gaccattgac ttgagaaata cagttcgatg acatttcaat ttgagcaaaa gatgaggtgc181 tcaagtatgg cattctgtct cattaagaca gaattcaaac tttctgcaaa gaaaaagtcc241 ctcatttctt ggtgtgctcc aggacctcca tattctttag atttggaggc accgcccatt301 gaggagctgg ctgcagtgtc agcgtgagaa ggaggaggaa ctccaggcca ggggcggggt
361 actccagatc cactggcctc aaggacccct cagatggagt cagtgtggga gcacagagcc421 tggcagctcg ggaggcctgn gtggagggga aaagaacagt gctgctctaa gacccagccc481 cctcgtgggg gagagagcag cagggcttgg ctctcagctg catggcaggt gtggaaaagc541 tccttg<210>6<211>350<212>DNA<213>Homo sapiens<400>61 ttttaatgtc ccaagttctt tataagtgga gaaaggcaga tggggcttta aaaatcactt61 ggtgcagcag aacctgaggc ccggcggcca gcgaaggccc cacggtgtgc ccgatggtca121 ggggctgcag tggatgggga cgtggaggct gcgtaggagg caccctggag gctggacacc181 ctggaggcct ctgggcttga gctttgcacg caggtcagat gttttttaca gtcagcttca241 aatggtgaag gggccctact cctaggtggt ctggaatgtt cccacagctg cctggtggtg301 gcccccaccc aggacccagc agggtacccg ccacaggacg ttcccgcact<210>7<211>524<212>DNA<213>Homo sapiens<400>71 tgcttattga aactgaaggg atgttgggaa agacagactg ggagctttct ctaaatttta61 atacagcatc agtgcttcct ataatgtcca ggttaggaga gaagcaaatg gagctttact121 aaggaagaga aagtgatcaa taccagtgag aaaggtgaaa aaaaaaaaaa acaaacaaaa181 acgaaaaaaa aacctaagca aattcagtga gaaaagaaaa agcagaactt agagtcctta241 cccttcaatt taaggaagga gagttattgc ctagcagaat cttgaaataa aatttcctta301 gaaagcccca gaaagttttg tgtgtattgc aagtccaaag gataaggaga acttctatat361 gctttcttct tatttccact gggcaaagta ctgctccatc aagactcagc ccgccatgag421 gctttccaat caactctcaa ccaccacaac agttagggct ttttcctcta tgttgcaaag481 cactttctgc ataactcaga atgcaaaatg tactcattca tttg<210>8<211>618<212>DNA<213>Homo sapiens<400>81 ttaagagttc tgaagtgatt ttacttttat ttccttcact ttaagccaat tatgaaattt61 cagtgatttc tggggtaggg gggtaaggca gtgttaagaa tcattggggc tgtggcccag121 tcagcctgcg gaggtgcagg cagggtgggc cctcatgggg cagccggagg agcggactgc181 cccgccggca ggtaggtcat gttccgagag cctccgcggc ttccaacttg cacagctcca
241 tcaggccgtc ccccgcggtg gccagtgagt tggcaatcag ctcggttgcc ttggagtcgc301 cctcagcaga gatcacggct gcctttttct gctgctcaac ctttttctgc tgctcagcct361 ttttctgctg ctcagccttt tccttcacaa atctggccct ctctgcttcc tgctgagcca421 cctgtttggc ttccactgct tctgtgaact cctttccaaa ggtcagatat gtcaaggaca481 cgtcgtccag gatgagcccc aatgtggctg cttgctccgt aggtcgttgc tcacctgcct541 ggagaccagc tctctctgag tgataacttc tccagcatca aagcgagcca ccactgactt601 tgagatcttc gtcgtgat<210>9<211>517<212>DNA<213>Homo sapiens<400>91 aaacttttaa tatattatttt tattgtaca tgttcacctt gagattatta cagacatttt61 tcaaataaaa agtttttgta aaaaaaaaaa aaaaaggaaa catttcctga attatcactg121 gatagttgaa acaaagaaat aaaatatata aatatgaagg tcattctcca agtattagaa181 cagaatacgg atggaatcac ttcagtaaag ttattcataa acatttgcat ggttacccac241 atactgtatc accttccaaa aaatcaaaca catgagagga aggggaacct caaatgagat301 gtcttttctc catgggattt ggtcaacgca tatcagtaag ataatttctt tgctatatac361 acaacataaa catttgaaaa tgcagaatac attgtgtaaa ttaaaaattt tgtcagcttt421 gttgtatggg gagtaactac acagaccacc cacacaaaca tttaaaatat actgtgtata481 tattatctta caaaaatcgc atgtgtaagg gaataag<210>10<211>343<212>DNA<213>Homo sapiens<400>101 tttttttttt tttttttttt caaacaataa ttcatgtatt taccaagcat ttatcaagca61 ctttttnnaa ggggnnttta aggtttgggc nnnnngcann aaaactgggt ccttatgggt121 tttgcagtag ggaaaacagt gaaggcaacc atctcacaaa ctgctggagt tgaaaccacc181 aaaggggaca ataaaatgtt agttccattt gatcaatgaa accaaagagg actggggtga241 aattactttc ccacagtcac aagtagtaaa actgggattc aaacgtgtgc tactgtaaca301 tgagccccta accacaggag tctttgtggt ttttaaaagc aaa<210>11<211>476<212>DNA<213>Homo sapiens<400>111 tttcagaggt agaaaggatt tactcttcag ccatctggta gagccccaag aacaagttac
61 atgtggacaa agggagggag aggtatcatg gtgattaata aattcaaaca aagctgaatg121 ataagacccc aggatggaat acagtctgag aaaggcctgg gcaaagggag gcagagggac181 tgaaggaagc aggtcaagga agatacaccc atggtagcct aggaaattgg cagtagtgtt241 ggtgggtggg gaaaaggagg gtttggctgt atgcctagct ttcacaaggt aggggatgag301 gaagatgatc atgatattgc acagaggacc caacactgaa ttgtgggagc tagaggttgc361 ttgaggcaaa gtaaaaaggc ttcggacact ttcaaaagga ggctggccaa aggcgtgatt421 agggactcca gtaatggaca tactagaggc cctcgggcac ctcaagccct gaatgg<210>12<211>445<212>DNA<213>Homo sapiens<400>121 catggcacaa ataaataatc tttaatttca ataaatagct ccccatcaca ccagacatta61 tgctgtggaa aaagatacag aagcatccaa cagagagaga ggaaagggag agagagagag121 agatctattc taactacact aattccttag cacgagagac gtgacattcg ttcacagaca181 aacacagaaa gatcctcaga gtatgattaa ggaagaagat catcatggat ggggtgtgat241 agccatttta atccatatat caggtctttg tatgtctttc gtgggtcaca atcaccatga301 ttcggtcaaa aaatgttact tttgctctat catatcctat ttacacacac acacacacac361 acacacacac acacactact tttatccaaa gtgggatctc tgacagacag gaggacactg421 gttggactct cactggacag gagag<210>13<211>555<212>DNA<213>Homo sapiens<400>131 cagtaagaat atagattttt attatacaat taacaaatta tatttggcat cacttaatac61 ctaaaactcc tctgggagta ataataatga acatttctca aagccatact atattcttac121 actatcctat gatgtataca aatatacttt cagtgaattt tcataacaac tcttccaatt181 ttacagctaa gaaaactgaa gcacagaaag tcaagaaatt tgcccaaaat cccacagcta241 ataaatggca gatctagaat tcaactctca ggaaatctga ttctagagcc cagttctgaa301 ttcctatgct atttttcaac atcttccatt ttctctccct tcttgagtca attttggtat361 ttttcatctt catagaaaat catctatttc atttggatgt taaaatttat tggcatgact421 tgacttcaaa ctntggtgtc aagtaccaga atttatatca gaaataaaac tatttttagc481 cctatattcc ttntcctata tcatgagcct tataaattca tcccagaggg accagtacac541 ccagatgttg gactg<210>14<211>476<212>DNA<213>Homo sapiens
<400>141 caccttgaaa caatttaata atgtattaca ttatagtagc atcacagcag cagtcaataa61 tgccacttta gacaaaaatc agtatttcca ttatgcattc tgtgtataag aattcataaa121 tcggtaaaag tcattctaag aaaacttggc aaatacagct ttggactgga attggcattt181 ctttgtctac ttttccttcc cctagattct ttgttttaaa ctacagtatt catattttaa241 aatgttttaa attattttaa gacgttaata tagcagttac atttgaatag ttatttgaaa301 gtgactgtaa gataaagttt tagagaatct attatggata gggttgattt acattttcac361 attttctaaa aatcagcttt ggttttagaa ctgattgttt ttcattttgg gaaaacctac421 caggtttaat caattactnt aaaaataatt atcatatttt gcagtcttta aatagg<210>15<211>503<212>DNA<213>Homo sapiens<400>151 tgttttgttt tctttttctt tttttccttt ttctctgttc tctacaaatc atttgggtct61 acatccagct tctcccagca tttactcaat atgaaacttc cagttataga ttaatttgaa121 ttctttgcac taattgttta aaagaccata ggaacctcag accatccctc ttcatggtct181 caagagctcc cagaatcatc ctcttggtaa gctaagtagt tattagtaag gcccagggac241 aggactgaga agtaacatct tctatttttt atttcagttt tcctttaatt tggaagcaaa301 caaacaaaaa acccttcagc ttagggtttt ttcaattgtt aaagtaatac tgagcttcag361 ttaaggctat gaaagaaaag aaaaaatttt aaaaagtagt aacgtgtttt tttcaagata421 ttgtgggtaa aaacataata aaaagaaaat aataattact tggaatcatg attaacattt481 tgatgtattt ccttccaatc ttt<210>16<211>518<212>DNA<213>Homo sapiens<400>161 gtgacaataa aatcaaactt taattgggga gccagataat attgcctttt ctttccaaaa61 ttttaattta tactttttag taaaaaacat aacacatttc tcattcattt aaccaattaa121 tccaaaggaa aaaagtgaag tactataatc acaggtacac taacaaacac ttttacaaag181 acattcagaa atatttgcag caggttttat aagactctga tataaaacaa agtaaaataa241 aacgttttga aatggcaaaa tcggtttacc tagcctgaat tgcttcaaag aggcatttgc301 acttcttgtc ctggcttaat aaaacaaagt aaaataaaac attttgaaat ttcaaaatca361 gtttagccta aattgcttca aagaggcatt tgcacttctt gtcctggctt aaaaacaaac421 cctgaaatta ctttctttta tccaaatcaa ttgaccatat taatctgcat aaagtctatg481 aggatacgtt ttgataaatg ctataaacac aaaattaa<210>17
<211>593<212>DNA<213>Homo sapiens<400>171 gcggccgctg cttccgcgag ccgcgcttcc tgctcaacca ccagcgcacc cacgcgcgca61 tgcccgcgcc gcacccgcgc cgccccggcg tcttcgggga gcggcggccc tacttctgcc121 cccgctgcgg caagagcttc gcgcgcgagg gctcgctcaa gacccatcag cgcagccacg181 gccacgggcc cgagggccag gcggcccact taggacgcgt gctatgatgc gcccggggcc241 tgctgccgac ggctgcttcc cgccccgcac gtgcgtcccc gacccctgga gatggccctg301 ggccgcagct ccctctctag atgggatccc gggagagggg cagcgctggc ttgggctctt361 tgaggtgtgg gccgccgcca ggctccggcc gccccgctcg cgttcctccc tcgggaccct421 ctggctggct gcgcacagct gctttcggct tctggccatg gtctcctctc tgcccattcg481 cctaanacca gtgcggagtg ggccagacct tagaaccgga tggtgcgaac cattctgaca541 ccggacacaa ggattgattc cttgaggccg gttggactgt cccccgccgt agc<210>18<211>473<212>DNA<213>Homo sapiens<400>181 ggacacagtc ttgctgttgt cgcctaggct ggagtgcagt ggcgcaattt tggcctactg61 taacctgttt cctgggttca aacttctgcc tcagcctccc aagtagctgg gattacaggt121 gccactgtca ccacgcctgg ctaatttttt gtatttttag tagagacatg gtttcaccat181 gttggccagg ctggtctcga actcctcacc tcaggtgatc tccccgcctc ggcctcccaa241 agtgctgaga ttacaggtgt gagccacagc gtccagcccc agagttttac ttcttaaaaa301 taatttattg taccacaagt agctattggt agagatgaaa gctaaagcag aatgatggaa361 tacagttgat gaaactttaa gaagcaatgt agcatgtaat cctcatagct tactgtcagg421 atgaagcctt tatgtttaca tccaagaact gagttcactg atgtcaacac cta<210>19<211>500<212>DNA<213>Homo sapiens<400>191 ttttttagaa aaaaatagtc ttttattttg ataaacatgt agatagcagt aactataaag61 gctagtatac ttaatatgta cattatatct tttaaaggag aatctggggc atttccttgt121 gttatataca ataacagaat catggtccct tcaggttatt gttcttttgt tttaatacat181 acatcttgag tttcttctgc atccttgttc tccttgacat cagccactct gtcaaatctc241 tgtattccta agtttctttt ctaagagtta ttttcctgca gaaattctcc ttttatccaa301 atcctggtca agcaggtgtc aaaaggtctg atgctgttag tcagggacat caattctctc361 ttttcacatc tcattacaga ggccttaagc tcagataccc actggtgata gacagggcag
421 gtcacacatt gagagaagaa ctccttacag tacacatggc cagggaggag tggaccccca481 gcatgacatt gctctgagag<210>20<211>527<212>DNA<213>Homo sapiens<400>201 tttaaagaaa gtttctttta tgggtttatt ggtcctttaa gttaattgaa gtaaagaaac61 tttaaattta caagctaata tttatcaagc aatacaaatt cttttgttta cctcaaaatg121 taatactaat tccatagtaa acaacaattt ttttatacac agatatatga aaaaagtgga181 caaaaacctc gtgccagcat caaaaagtca acaaaaaaaa cgattctttt ttccatttta241 caatctctgg tttccataac aaacaaaaaa aagggtgaca ttctttttaa agtttggtag301 cttcatgaaa caaagacttc catctgtaaa taaatcactt tcttatcaac acactctctc361 aagcactttt tttatcacag cttttgtttt ggcataatgg cagcatggag agaaggtaac421 atttacacgg tggactacat caatacctca gaaggttttt attttaaaat tttaaacgta481 aagaatgact tttaaaaata agatgacagt ccataatgtg gacatgt<210>21<211>465<212>DNA<213>Homo sapiens<400>211 ttaagtttta attaaatttt tatttgacca agaaaaaact gtctagaatt gcctgacatt61 taaaatccac ttaaagcaga ttagcaactt caagcatcac aattattcag cataaagagc121 ctttgttgaa acatctttct catttattta gaagaaaaca tctagaacag aaccaagcaa181 aacttttgta tttttgtaaa aaatctcaat tcctgaaact actataaaca atctactgaa241 actgtattca gcaagggaca gatttacctc aaggccatgt tcacctgcaa cctgctgata301 agtctgttaa aaaacaaagc aaaaaaaaga gtttttaaaa attagaccac tgtagccaca361 atccctaaca ctggacagtt aaaacaaaag ttatcaaaat ctgttctttt cttagcttta421 cctgtatcac tttataactc ctgagaaagt cattcacata agctg<210>22<211>525<212>DNA<213>Homo sapiens<400>221 acatgagata ttcaacattt tattataaaa caggctttct gttagatgat tttgctcaac61 tttaggtgtt ctgagcatgt ttaaggtagg ctaggctaag ccatgatgtt tagtaggtta121 ggggtattaa gtgcattttc aaattaccat attttcaact tacaatagtt tcaacgggag181 gtaaccccat cgtaagtgga ggaacatcta gtgcctggca cagagccggt tctcaataaa
241 tataactctt ctccatcttc ttcaaacctc aggccaggtt tcagtgacct cctctcactt301 tctaagatta tttttgcttg ctggtgggtt tactgtcatt tttaaccaca tctaacctac361 cttaaaaaag tgtatggatg ggggtgccag gtacaaagac ttagcataaa gaaaacgacc421 atttactttg aaacataaac aaaggccaat taataattta tgagtctgtt ccttgtccct481 cgagttttta gcaggcatgg aatcattcca caggtcatta tttca<210>23<211>539<212>DNA<213>Homo sapiens<400>231 tttttttttt tttttaaagg agacagaaga gctttattta agttctctaa ataaaagttc61 aaagataagg aaaaattact ttaattccac caataaaaaa attacaaaca agtagccatg121 tattctacac tagatgaaca taagtgttta agcacaagca aggtgaataa gaaaagtttt181 catttccctg ggcgaggaac atcttctccc tttgtcctca gcatcagttt tacttgcccc241 aagtcagtgc atcacaagct ttgctgctag aatacgcctt aagatgcagg gcagcaagca301 atcctccaat tgttccaggg tgtgatcttg ttttactttg ttaattatta ctattttaag361 atttcaatag ttttgggggt acaggtggtt tttggttaca tggatacatt ctttagtggt421 gaattttgag attttagtgc acccatcact ggagcagtgt acactgtacc caatatgtag481 tcttttatcc ctcaccccac tctcaagctt ccccttgcaa gtccccatag tccattata<210>24<211>504<212>DNA<213>Homo sapiens<400>241 aaatgggtat atttgtgtct acaacttttt tttaattata gcaatagaca atgtgtatat61 agatacgtat ataaagcaat ggaagtggca ttgttttcat actagtctag aatattaaaa121 aatatgtaat atgtgatcag caaaataatg gccccccaaa gatgtccaca gattcctaga181 accttacatg aaaacggtac atcatgcatg tgattcaggt aaagaccttg acatgaggag241 actaccctgg tgctatggaa tgaacgttgt gttccgtcac tgttcatgtg ttaaaatcct301 aaccctcaag gtgatggcat taggaggtta agccttacgg gagactctgc cctcatgact361 gggattagca ccttattaaa ggcacaaggg agcttgtttg ttcctctcac catgcgaaga421 cacagcaaga aggaagcctc tatgagaaag caggccttca ccagacacca agactgccgg481 caccttgatc ttgcacttct cagc<210>25<211>601<212>DNA<213>Homo sapiens<400>25
1 tttttttcaa aattcaataa ataggtttta ctatgtagat gaacaaagct ttaaagtgaa61 taaggacagc agtgaagggc aagtcaagga tagatacttc acaagccaac tgaatgaaga121 ccatggattc gttagctctg agccgtttaa catttcatat tatacttagc aggaatgtat181 gacaatatta ctttgatttt ttgctgggcc cgcttaatta ctgcatccag ttcttccttt241 tttcattttc ttctcgaagt tttctttgta gctgtatctt ttgatatttc atgtgatcta301 cactctgctc tagttcatta gcacgtttct cattttggat tgacagtgat gccagtttct361 tactatcttg ttgcttcttc tgtaagacct gaactctcag ctttgcagca tccacctttt421 tacgaaactc tttctgtaat tttaccttca ttgcaacatc agaaagatct ttgttttcca481 gctcctgtag ctgcttttgt gtttcagtta gttcgacttt tgcctgttct gcatcatgct541 ctagctttgt tactttcaaa gtatactgct tgcttacaga cttggcatcg ttacctgttt601 t<210>26<211>511<212>DNA<213>Homo sapiens<400>261 tgatattatt tatttctttt ataaattttt aatcaaaaca ataaaacaac atgcttaatt61 attaggtggt agttacacag gggctattgt ttctgtgtat tatttgtatt tagtaaaggt121 ttaaaggcat aaaatagaag aaaatttgga aaaatatttg gggtgaatga gacaattcag181 gaacaaatac agaaaactat attatcatat agcaaaaagc tatttaaaat agagataagc241 aaactatagc tcacaggcca aatgtggcct tctgattatt ttttgtaaat aaagttttat301 tggaacacaa caatgtccat ttgcttatag gtagaatttc tcaacctcag aactactgac361 attctggatg agactttttt tggggggtgg gggcagttgt actatgcatt gtaggacgct421 tagcaggatc cctggcttct gctcaccaga cgccagcagc acccaccctt catactgaaa481 accaaaaatg actctggaca ttgccaaatg c<210>27<211>2240<212>DNA<213>Homo sapiens<400>271 gtaggccttc ggaacagcgg aagagcagtg tcgggccatg gccagggccc gcaggctgga61 gctggccgag gccctggcgc tggggccagg ctagaggcat gcctgctatg cgctcctgca121 cgcgccggac cctggaattc tctctggccg cctccctctg cgctacgcca tactgatgca181 gatgcgcttt gatgggcgcc tgggcttccc tggcggattc gtggactcgc aagacagcag241 cctggaggac gggctgaacc gtggtctgct ggaactgctg ggcgaggcgg cggccgcctt301 ccgcgtggag cgccctgact accgcagctc tcacgccgga tcaaggccac gtgttgtggc361 ccacttctat gccaaatctc tgacgctcga gcagctgttg gctgtggagg ccagcgcaac421 aggggccaag gaccacgggc tggaggtgct gggcctggtg tgagtgcccc tgtaccctgc481 atgatggtgt gggaggcctg cccatctttc tggagaattg ctttattggt gctgcctggg541 agcagttact ggataccctc caggacttgg gagtggtgga agctggtgct ttctcaggac
601 ttcagatccc agttcgtctg tggcagccct ctgtggactc agggaacctg agctgaggac661 tttggtacaa ggaagggaat gttttctctt ccggacctaa agacgatacc aaattaaaag721 aagacatgta tatcatatgt tttgagcaga ggaccctcca attcatggga tcaggggcaa781 aaatccacag ctcatcccag gggccctggc aggttctcag agcctacctc ttccttgtat841 atttgcatac agcctggtaa cccccaggca tgcatatgta caatgtgacc acacacagcc901 tgacatcttt cacctgtcaa gtccaattta accaggaatt ggcatttgtt accataccct961 gcctcttgct ttgcagagag cttctacagc aaggctgagt ccttcccaca gtcctccatg1021 cctgctcttt agggtctacc ccagcccatg ttttttggga aggatagaga agcagaggac1081 cctctttgct ttcctgaaag tagccatgcc cttgctggag ggttcaccac agcaattggt1141 gggaaagggt cttaaccaat ctccaacact tttcttctcc tgaaactgag aaggggtaag1201 gaagtagcta ggtccccttg gacggagcat ggacatgaga cctgtgagta ctggtgtctc1261 tccttcagag ctttcatctt tggttcgctg tagactctgg gactatcaga agagaactga1321 cccatacctg tctagccctg cccttccaaa aaaagagcct cccattaaag aggagttaga1381 gctgggcctt gaggctcttc acttctttca acagatattt aatatgtacc tactaaatgc1441 caggtactat aaaaggtata gagagccatc cttgctctcc aagactccag ggaagggatg1501 ggcaaacatt ttcttaaaag ccagatagca aatactttaa gctttgtaaa ctacatagtt1561 ttttaaaaaa tattttctaa cattgactaa taaaaattgc ctatgtttac tgtgtacaaa1621 atgatgcttt caaatatgta tacattgtgg cgtggctaaa ttgagctaat taacatatgc1681 attacctcac atatttatca tctttcatgg taagaacaca aaatttactc ttagcaattt1741 tcacaaatta atgcagttta ttaactatag tcaccatttg tgcaacagat cttttgaact1801 tatttctcct aactgaagtt ttatattctt cgatcaatat cttctcaatt actgttccct1861 acccccaacc cctagtaacc attattttac tctctgtttc tatgtgttta acttttttag1921 atgccatata taagtgaaat catgtggtat tttcacctgg cttatttcat ttaacatgtc1981 atctaggtta atccatgttg tcacaaatga cagaattttc ttctatttta aggccaaata2041 gtattccatt gtgtatatat accacatttt ctttatctgt ttatctgttg atggacactt2101 aggttgattc cacatcttgg ctattgtgaa tagtgttgca gtgaacatgg gagtgcagct2161 atctctccag atcctaattt caattacttt gaataaatac cctgtaatgg gaaaaaaaaa2221 aaaaaaaaaa aaaaaaaaaa<210>28<211>2993<212>DNA<213>Homo sapiens<400>281 gtgcacgcgc tgaaaccgga gcgcgcaggg cgcgccaggg tgagggggac gggggcgggg61 tgggggcagc cctttcccag gcggtagcgg gggcggcgtc gctgttgccc ttttaagccg121 cggggccgcc gcctcctgcc gctggagtcg gttacaaagg aagcgccacc caggccgcca181 cacgccgagg cttccgcgcc cctcgccatt ttccagcagc gctcgacgag gcggagccgc241 gagagcgcgg cccaggccgg ccccgcgggg cggtcgcggc cgtgacggcg gctccgggcc301 cggctcccct tccgcgcccg gctccccttc cgcgcccctc ccgccggaga tgaggggaag361 atgtccgtgt cagggctcaa ggccgagctg aagttcctgg cgtccatctt cgacaagaac421 cacgagcgat tccgcatcgt cagttggaag ctggacgagc tgcactgcca gttcctggtg481 ccgcagcagg gcagcccgca ctcgctgccg ccgccactca cgctccactg caacatcacg
541 gaatcctatc catcttcttc accgatatgg tttgtggatt ctgaagaccc aaatctgaca601 tcagttctgg aacgtctaga agatactaag aacaacaatt tgaatgggac aacagaagaa661 gtgacttcag aagaagagga agaagaagaa gagatggctg aagatataga agacttagat721 cactatgaga tgaaggaaga agagcctatt agtgggaaaa agtcagagga tgaaggaatt781 gaaaaagaaa atttggcaat attagagaaa attaggaaga ctcaaaggca agaccattta841 aatggtgcag tgtctgggtc agtgcaagct tcagatagac ttatgaaaga gctcagggac901 atatacagat cacagagtta taaaacaggg atttattcag tggaactcat aaatgacagt961 ttatatgact ggcatgttaa actgcagaag gttgaccctg atagtccttt gcacagtgat1021 cttcagatct taaaagaaaa agaaggcata gaatatattt tgcttaactt ctcttttaag1081 gataactttc catttgatcc tccatttgtt cgagtggtgt tacctgttct ctcaggaggg1141 tatgtattgg gtggaggagc attatgtatg gaacttctca caaaacaggg ctggagcagt1201 gcctactcaa tagaatcggt catcatgcaa ataaatgcca ccttagtcaa aggcaaagcc1261 agagtgcagt ttggagcaaa taagaatcaa tataatctag caagagccca acaatcctat1321 aattccattg tacagataca tgagaaaaat ggctggtaca cccctccaaa ggaagatggc1381 taaatatgtt gactgttgta tgtttggact aatgttgctt taaagaaaat ctttccaaca1441 tgcagacaaa agctttgagt gcccctatta cagcagtacc gaagatgtta gttaatagat1501 attttagtgg ataatctgtc atctgacatc cagtataagt tacagccttc gcattttgct1561 cattttagat atcttggact gagcagtggg gcctttactg tatttttcct gataaataca1621 catactggcc actccttatc tctttttctt gaaaagtgaa ctttttaaag cagccaagtc1681 aacatcaggc tactgaagtt gaggctttag ggtaactttc ctatattgag cccatgggtt1741 acaaggattt gcaatatatt gttccattta cagccaatac aggtttaatc gatgttcaat1801 attggtttag gaaatttaag gccttctaaa tcataatagc tctttcatgt ctaaaaccat1861 tttatgatat tgccaaaatg tgataggaaa cctactcatt aaattgttaa actttttaat1921 gactatgtga agatatgaat tgtttcctga agataatact cttaattgag ttgtattgta1981 cttcttaggc aaagcagtgt aaaactgtat cagttaaggc ttgtgagtag tgatttccac2041 tggggcatca gagtcttggc tgggctgaat ctgctgcttg ttggttcagt gtttcttatg2101 aacaagagcc acagtacaga gcttcaagtt atttaaaata ctaagtcatc ttacgtttcc2161 attttattaa cgggatgttg caatcgtttg taaactaata aacttataaa gtgattggca2221 caaagactcc ttgagcaaaa gctgtgcagt taagtacaaa aagatactta atttggagac2281 tcttacagta atttttgcca tgtcaaaaca atggctttta cattgaaaga ttaatagaaa2341 ctctacatat gttaattttt ttatagaacc tgactcaaat caaggtactc tccattttat2401 tgccttacct gaatcagtcc tttttggttg gtaatagatt tttttataca cccacgtttg2461 atttaaaagt aaattctagt tcttaagcac ttttaacaag aaatccagaa gcacattttt2521 ctgcacaaac aagttacaaa gttcaaaagt gtttcttgtg cattagcttt gagattcagt2581 ttttaacttt gtaaaccaca tctgagagac ttgtcatttc tacattgtgt gtgtttaatt2641 tcttttgatt ccattttggt taagagagca gtaaatagat tttctggtat tcttgttcac2701 ttgattacat ttgtataaag ttctgattgc cagttgctca gataacaagt aacaaggcag2761 aattctttaa atcagtaaag ttccttaagc ctaaggctaa atcttgaata cattgttgaa2821 ttctttaata tcctgatggc aagcagactg atagctgcac atttggcatg ctttgtttaa2881 tggattttat ttttaattgc agatttattt ggcaatgtac agtaaatttt gtaaacttgc2941 atcaagttta tgaataaaga accatttaaa aaaaaaaaaa aaaaaaaaaa aaa<210>29<211>2309
<212>DNA<213>Homo sapiens<400>291 acagtatata ttaccaactg tttgtagtat cctataactt tgttcatact taagttgtat61 ctctttcctt tttgtacttc atggcaaaat taattttcta aaggattcag agaggcgttt121 atattttata acatttatta gaaataattt gttccaccac tagattactg tattttatgg181 aatctaagct gtatatgtgt acatttgtat acatgtatgt gtaaatgtaa gcctgacgtg241 ggttcagatt ccagttttgt catcagcaag aggcctcgga cccatgactt actgcctctg301 agccttggct ttctgtgtat ctctgccact gggataatgg tacacataac agtggagtgg361 ggaccaattt ctaaaactca tggcacctag tctttggtcc ataaatggtg tttgttattt421 tagacactga gaaaaatctt attcacagaa gttgcagatt atttctgcta gtcacttttt481 tatttcttga gagctgccac tcatttaata tttctcattt atgagaagag acttgactga541 tttagataaa agacgaatta aggaaaacct ttgaacgtga gtagacaagc ttcatatatc601 tgtaaagtga atattgaatg gtagcttgag tcattcgtac ttatcacaga aaagaattga661 actcccagtc atacacctgt aactgaaata ccaaactgtc ttaatggaga tgtaatgaag721 taagatggtc acatctttaa ctaaacttat aaaaattggt cagtttgatc attttcattt781 aaatgagaaa catctgggga cacattcatt cattcaacaa atatgtattc agtatctact841 gtagaagaag ctctgagatt ctgcggtaaa tagacatgca aagcccctgg cctcatggaa901 ttcctattct ggaaaggaga gacaaacaaa taagcaaggg aatcttagtg tgtgtggagt961 tctctgaagt aaacagtgtg atggtgtaga gagcacctgt gtgagtctga gatgagggga1021 tgtgatgttg tggacttcag ccaggttttt ggcagctagt gtctgtccct ttttcagtag1081 ctccagtctt cttatgaaga atgagttctt tctgtggtgt gtgactttag tgggagggca1141 gctcttgctc tatgcctctc agttgccttc cactgtagcc agtcctgtaa ctggtctggg1201 cacactcggt gggggatcca tggtagctcc ttgccatgaa gatgtgggta gaggagggtt1261 ctgtggggcc tcggaggtgt tcaacgtttt ctagaagtgt tccttacggt agagtgccaa1321 ctgtacatct agaagaaagg acagaattag cagattgatt caggccagaa tcatccgtga1381 gtgctatttc aagtgtaggg actctctgat gagtaacagg atttacatca tcccaaagca1441 tctccccaca aactactcat tataaaagca aaaatagcaa ctgcacaatg gagacaccgg1501 atcccacctt cactgtgtgg ttactgccat catcaagagc agaagggcct cctgggtgtc1561 tgtggagccc tggtaagttc acagcttcac aggtggggtg ttgccaaaga ccacacaccg1621 cactgctcat gagcacacat cagaggaccc accgccaggg ccactcggtc cgataactga1681 agaatgttat tgtcacgaaa accaaagaaa gactgagcaa ctgttttccg tctaaggaga1741 ccaaaggaat gttatggcta aatgcaaaaa tggtcctgga ctggatcata cccttgggaa1801 atgttataca gaacattatt ggggcaatga cagaatcaca gtgttatttg gaattattat1861 attggaatac aatgttaaat ttcctgaata taactgtgct gggattatgt atgaaaatat1921 ttttcttaga aaatacacac tgaagtatta aggaagtaaa gtagcatatt gtatgcaacc1981 ttttctgcaa tggtttagga aaaaaatgtt caaagagagg aagagaacaa atgtgacaaa2041 atgttgaaaa tcggtaaacc tgtaggccag ctgcagtggc tcacgcctgt agtcccaaca2101 ctttgagagg ctgaggtggg cagatggctt gagctcatga gttcaggacc agcccgggca2161 acatggtaaa accccatctc tacaaaatta ggaggttgag gtgggaggat gatttgagtc2221 taggaggcgg aggttgcatt gagccaagat cacaccaatg cagttcagcc tgggcgataa2281 gtcttgtctc aaaaaaaaaa aaaaaaaaa
<210>30<211>1625<212>DNA<213>Homo sapiens<400>301 acagcggggc gtcacctcgg acatacgaac agcgggcggt gttcccggct ccgtggggcc61 gtcttcccgg cgagtccgcg acggtcgccg cccacggcga gatgaaatct cgctctgtcg121 cccaggctgg aatgcagtgg cgtgatctcg gatcactgca gcctctgcct cccgggttcg181 ggcgattctc ctgcctcggc ctcctgagta gctgggatta caggtgcgtg ccaccacgca241 cggctaattt ttgtattttt agttgagacg gggtttcacc atgttggtca ggctgttctt301 gaactcctga cctcgtgatc cgcccgcctc ggcctcccag agtgctggga tttacaggca361 tgagccaccg cgcccggcct gttttttttt tgttggtttg tttttgagac ggagtctcgc421 tctgtcgccc agcctggagt gcagtggcgc catctccgct cactgcaagc tccgcctccc481 gggttcacgc cattttcctg ccgcagcctc ccgtgtggct gggactacag gcgcccgccg541 ccacgcccgt caaatttttt tttttttttt tgtatttttt ggtagtgacg ggtttcacca601 tgttggccag gatggtctcg atctcctgac ctcgtgatcc gcccgcctct gtctcccaaa661 gagctgggat tacaggcgtg agacaccgcg ccctgtccgg cctgttatta tacatatgat721 tgatatctcg tgactcccta aaatgaatac aaccaagctg tgccccgacc accttggacc781 cacgtcgtca ggatctccgg aggctgtgtc atgggcgtcc ttaactttgg gaaataagcc841 tcctgaaatg attaagactt gtcatttttc tcgattgaca ttgataagca ctgagagatc901 ctcagtgaag gtaacccggc ctgcagcagt tcgcctattg atcctcggta ttggcttggc961 accttattgc ccaaactggt aggatgattg ctgaaatctg ggaccttgtt cctccaggga1021 tccctgatct tccaacgttt ttcggtgggg ggtctgaggt ttatttgctg ttaaaaaact1081 ccttttttgg gagttcccac tcgcttcatc aaggaaggca agcctgcctg cttctgcatc1141 gacagatagc cgtcttcagc ttgagcccca tcactagatg tgtcttgtat aactatccca1201 cagctactaa gcagctggct gggactcagg atcaacgttg gcctgactct gaagcccatg1261 cacctccata gaccacacta ctcagccagg tttcctggac cttcaagggg ctggagtgac1321 acttctgaaa taggagcaag atttttttga gaatgtcatc ccatccatcc attactcttc1381 accattggaa ataattcact ccagggaatg gttctgaagt ctcttgggaa aagcgaaaca1441 atttgagaaa attgctgagc attgggttta ggaaattaat gtccaacatt acatttttaa1501 tttatcatag gagaaatttg aaaacaccta taatgcactg atcatatctg caaactcttt1561 gtattttttg tatttgttga ctatttatga tttttaatat agacaaagtc aaattactgt1621 tcctg<210>31<211>2467<212>DNA<213>Homo sapiens<400>311 atctctgcaa gacaaaagtc agaaatggct tccctgggga ctgagagccc atagttctct61 tcccactgct gcttctaccc ttttgttttg cttggctctc taaaattgtc tcagctccag121 ctcttctcac agacagtttt atcatcttat catttttggc acaaaacaaa ctatgtttta
181 ttcagtttac caagaagatg gagtcttctg atgtaaacaa aagactggaa aaactctcag241 ccttggatta taagattttc tattatgaaa tacccggccc aataaacaag acaacagagc301 gacatctagc tatcaactgt gttcatgata gagttgtttg ctggtggcca ctggtcaacg361 atgatgcttg gccttgggcc cccatttctt ctgagaagga cagagccaat ctactcctcc421 tgagttatgc tcaaggaaga ctagaggttc tgagttctgt ccgcacagaa tgggacccac481 tggatgttcg ctttggcacc aaacagcctt atcaggtgtt cacagtggag cactccgtaa541 gtgtagacaa agagcccatg gctgacagct gcatctatga atgcattcgg aataaaatcc601 agtgtgtgtc agtcaccaga ataccactaa agtcaaaggc catcagctgc tgcaggaatg661 ttactgaaga caaactgatt ctgggctgtg aagattcttc gctaattctt tatgaaactc721 accgtagagt gactctctta gcacagactg aacttttgcc ttcattaata agctgccacc781 caagtggtgc cattctgcta gttggcagca accaagggga gttgcaaatt tttgatatgg841 ctctatcccc tattaacatc caactgttgg ctgaagaccg cttacccagg gagactctgc901 aattcagtaa attatttgat gcctccagca gtcttgttca aatgcaatgg atagctcctc961 aggttgtttc tcagaagggt gaaggtagtg atatctatga tctcctcttc ctcaggtttg1021 aaagaggacc tttgggtgtg ctgttgttta aactaggcgt cttcactcga ggacagctgg1081 gcctgataga catcatcttc cagtacattc actgtgatga gatctatgag gcaataaaca1141 tcctgagcag catgaactgg gacactctgg gccaccagtg ctttatcagc atgagcgcca1201 ttgtaaacca tcttcttaga cagaagctca ctccagagag agaagcacag cttgagacaa1261 gccttggaac cttctatgct ccaacaagac cacttctgga ttccactata ttggaatata1321 gagatcaaat cagcaaatat gcaaggagat tcttccatca cttgctcagg taccagaggt1381 ttgaaaaggc atttctccta gctgttgacg ttggtgctcg tgacctcttt atggatattc1441 attaccttgc actagataaa ggtgaattgg cactagctga agtggcaaga aaaagagcta1501 gtgacattga tgcagaatca ataacctctg gggttgaact cctgggaccc ttggacagag1561 gggatatgct aaatgaagca tttattggcc tgtctttagc acctcaagga gaagactcat1621 ttccagataa cctccctccc tcttgcccaa cccacagaca tattttacaa caaagaatac1681 tgaatggctc ttctaacaga caaataattg acagaaggaa tgaacttgaa aaagacatct1741 gttctggatt tttgatgact aatacctgta atgcagaaga cggagaactg agagaagacg1801 gcagagaaca ggaaatcaga gatggtggtt ctctcaaaat gattcacttt ggtctggtgt1861 aaacattaag aaaaaaaaga aaactgcctt cttcatatct aaacttttca tggcctgata1921 cagacttaaa atcccccgtg tggattttta acaagagaca gtatgaatca tataaattag1981 ttaagagttt tgagttgagt gtttgttttc aaaataaagt tttattattt caaaaacatg2041 cagcataaca gcaaagtgat cgtaagtaaa agtcttcaac ctacttattt tcaataatgt2101 aaaattttga aagtgtctta aattgtatgt taattaggtg ctacaaaaat agaatattta2161 aactttgttc tcccaggtac tttaaaaaag gggcagtagg gggcaggcat agtgctcatg2221 cctgtagtcc cagtgcttta ggaggccaag gctgaaggat cacttgagcc caggagttca2281 agactagcct gtgcaacata gtgaaacgct gtctctagaa aaaattttaa aaattagcca2341 ggcatggtgg tacatgcttg tgaagctgag gcagaagaat tgcttgagcc caggagttca2401 aggctacagt gagctgtgat cgcgccactg cactctaacc tgggcaacag agagtgaccc2461 tgtctcc<210>32<211>3079<212>DNA<213>Homo sapiens
<400>321 tcttaaacaa ggagaagtgc cagaaagccc ggaagcaagg aaggaaagtg accaagcatg61 tggcaaaatg gatactgtgg atattgcaaa caactctact ttgggaaaac ccaagaggaa121 aagaagaaaa aagaaggggc atggctggag cagaacggga acgagaacgc agaaaaacaa181 ccaacaaaat gataacagca aagccgatgg ccagctggtc tcgagtgaaa agaaggcgaa241 catgaatctg aaagaccttt ccaagattag ggggagaaag agaggcaaac ctggaaccca301 ctttactcag agtgacagag ctccacagaa aagagtccga tcaagagctt caagaaagca361 caaagatgaa actgtggatt ttcaggctcc tttacttcca gtgacctgtg gtggggtgaa421 gggaatttta cataaggaga aattggaaca aggaaccttg gcaaagtgta tacagactga481 ggatggaaaa tggttcaccc ccatggaatt tgaaatcaaa ggaggctacg caagatcaaa541 gaactggagg ctgagtgtgc gctgtggcgg gtggccccta cgacggctga tggaggaagg601 atctctacct aatcctccaa gaatatatta caggaacaaa aagagaatac tgaagtctca661 aaacaatagc tcagttgacc cttgtatgag aaacttggat gagtgtgagg tgtgccggga721 cggaggggag ctgttctgtt gcgacacttg ttcaagagtc ttccatgagg actgccacat781 cccacctgtg gaaagtgaga agtaagtgac atgcaggcac ctctctttca tcctttaagg841 gaccttccac ctgccatgcg cactctccag cagaatgtct gcttgtgtct agatggggaa901 ggagcaggtt catcgggtac tgggacccag cagtgtgatc cagagagcag tgggagacgc961 tggcagcagg aggtaaatgc tctctgtgct ctcagggaca cctcctctga tatgctgagg1021 ccagtgttgg ggacctttgc ctacatactg gtattgtcct ttccttgaag ttttgcagta1081 gggtctcctg ccccaggggg ttgaggaaaa aaaacaccag ttgtttatat tttgtgctta1141 ttcatacaag acaggaagca cggtcttagg cggatggagg cctgacgtcc ctggagttga1201 aagcccagtt tctgacacac aggcctggca ggcaggactc cttgtgctcc cagcagctca1261 gacagggaaa ggatagtccc cactcacggt gacaccacct tcctgaggct tccctcctgc1321 tgctaccact gatgcgaggg aaaggccagt ggctggagac cccatgtgca gttcttggat1381 actctgggag gtgttcccct ccctcccatg actgagccca tcagccattc ctgaaggaag1441 acagtggtag ccacaggaac ggtggccatt tccaagctcc ctgccctctg ctcacccatg1501 tccaatctct caggaccccg tggaattgca tcttctgcag gatgaaggag tctccgggaa1561 gccaacagtg ttgtcaggaa tctgaggtcc tggagaggca gatgtgtcct gaggaacagt1621 tggtaaatca gatgcaaacc ccaagccttc tcctttcccc tcacacgtga gaaccatgaa1681 caagcgcaag ggcaggggaa tgtactgaaa ggagcctgct ctaccgcgcc acacttcaat1741 ggcactgata gaacaccaca ccttgtttgc acaaaaaata actcagccat gacatgtctg1801 tttccaagag ccacacatgt tagagaaact tgaggaagaa gggtgtgagt cgtgtgctgt1861 gtctcatgca tgtgggctca ttctctccaa gctgcttttc atttacggcc tctttctttt1921 gcagaaatgt gagttcctcc tcttgaaagt ctattgctgt tctgagagct ccttttttgc1981 caagattcca tactattatt atattagaga ggcgtgtcaa ggcctgaagg agcccatgtg2041 gttggataaa atcaagaaaa ggctgaatga gcacggttac ccccaagtgg aggggtttgt2101 acaagacatg cgcctcatct tccagaacca cagggcctct tacaagtata aggattttgg2161 ccaaatggga cttagactgg aggctgaatt tgagaaggat ttcaaggaag tgtttgctat2221 tcaggaaaca aatgggaaca gttgactggt ttagtggatg ctgaaggcct tcaggaaata2281 tgctactggt tgccactgac ttcaaactga gagcacttgg gaaatagcac atgcagggag2341 aggcttttct ctgagcctcc ctcatccgcc caaaaacaaa tcctcaaaag aaatttgatc2401 atcatgaatc ccatccccaa gaatctcatc aaccagggaa gaggaactga gatcacaggg2461 aaggagattg gaggtgatac cagcacagac agactctcac ctcttctcct gaggtctgct
2521 ccagacaaca tttattactc acaagacctt tttcctccgt tttttttttg agatggagtc2581 tcacacagtc acccgggatt ggaatgcaat ggcgcgatct cagctcactg caacctctgc2641 ctcccggatt caagtgattc ccctgtctca gcctcccaag tagctgggat tacagtgcct2701 gccaccatgc ccagctaatt ttttgcattt ttagtagaga cggggtttca ctatgttggc2761 caggctggtc tcgaactcct gacctcatga gccgcccgcc ttggcctccc aaagtgctgg2821 gacgtgacag gagtgagcca ccacacctgg ccactcgcaa gaccttttat ctgaaaacca2881 gccaagcttt attcacgaca cacttcttcc cttcactctc ccacttctgt ggtcaactcc2941 ctgcagaact cccaaactgc cgttcttttc gatagctcac gatggtgtat gagtgtcaat3001 catctgaccc ttcttggagt ctcatatttc gtggaactcc tgtgcaaaca tatattatta3061 aatttttttt cctcctgtc<210>33<211>2750<212>DNA<213>Homo sapiens<400>331 atttattttg atgtcggcgg ggtgtgcaga tgaccctttc ctcacgccgt ctccgatatt61 ggtgttcacc aaaacatctt tatccagtag tgcaaaatgt catgctaacc ttgtcttagt121 ttcacgtctt taattctgaa acttgaaaca aaaacaatgc atcagtgggc catggagact181 tcttcttctg tgtatgggat actgtgtctt ttattcttct attctggtat gtcctccctg241 atttctagtc atttttgaat tatttataga cagtaaacat tgcccaatat attatgaaaa301 tgtgtttcca ctttattgtt gcctctgaaa acattttgaa cagaagcttt tataagcaag361 gcttgaattt ttctttttta tgttgtctta ggtttgtgtg agtttccacc tttgtgtaac421 acatttttcc ttttaacaga accattttat cactgtctct cttccttctt gggaaaaaga481 aaaccatctt tttcttttca tttgcattac ttacaactat gaattgattt atagagtgct541 aaaatatttg taaaatcgag cagattcttc tgtgtgcctt tttctcctgg tgatttattt601 aaatggattt ctaaggtgtt ttcccgtcag tattgatttg ttttggtgag tacgatttta661 tgtgcgctat ttgtctctct gtttctgaac tatagcaaca tttttgtgca ttctctctta721 ctctccactg aatgatgaac ccttgtgaac ttccaattgt tttctcttca tttcttaatg781 tttctgagaa gaccgacata ttatttgtaa gtgattttaa aatttgtctc cttttctatg841 ttttttcgtt gtcttcagag tgtcttcaga gtgcattcgt atttttgttg aattggcttt901 gctagagaac acatgatggc tttaagtatg agtgaagaga gtagacattt tttattcctg961 atttcaaggt aaaaacctaa agaggttcac ctgtaaataa aataaattgt ctttacaaaa1021 ccagatactc attccaagaa gatctctcta ttctagtata cttttttttc taaacataca1081 atagtatagg ttctcatact gtgagacttc ttatttcttt atacacaata tatttgaaaa1141 gtcattggtt ttttagtgat tactaatgta ctgttacatg gttttgaaaa gtgttggatt1201 caaagtttcc ttcatttaag cctctttttt ctcccttccc catataaaaa gttgatgatg1261 gccaggaata gaggttcaca catataatcc cagcactttg ggaggctgag atgggagcat1321 cttttgagcc caggagttca agaccagcct gggcaacata gcaaaacccc ctctctacaa1381 tgaaatgaaa aattagccag gcttgttggc acatacctgc tgtcccacct gcttgggagg1441 ctgaggcagg aggattgctt gaggccagga ggcggagcct gcagtgagcc gtggtcacac1501 cactgcactc cagcctgggt gacagaggaa gaccctgtct caaaacaaaa caaaaatggt1561 tcctcgactt tagttaggat ttgaaaagct tagaactgga aggcagcctc tgaaattatc
1621 aagaatgtca cagcagtttg tggaacacac ctgggtgggt agacccactt tttagacact1681 ctctagccca aatctgatca cagtcattct tcctcacctc ttagggtctc cctcacagaa1741 ggtgttgggg gccttggacg agatgttgat gggatgggaa taaacaggag caaataatgt1801 gcaggcgaag ttggagttcc tgctcagtct gggaggagaa ttgatttatg aatttctttc1861 tttctctctt tgtatctatc tcttctttct ctctctctct ctctctcccc ccagccacct1921 tctgatctct aatccattca tgtgagagaa gagtgatgtg aaaatggtca gcaatttgct1981 ttttctgatg agatcctggt gagagtcatg ttcaataaag tatttagtca cgtggggctc2041 cagtgatttc tctgtttaca agctcattcc ttcctcattt tctcagaact ttggtgttaa2101 cagcctgttt cctatttgta ggggctgact ttgacttagc agatgccttt cgtgatggag2161 gaaataacga cccagcacct cttaattcac ccaagctgaa gccaaatgcg aaccctgagc2221 agcctggatt cattggggat gactttgact tagcagatgc cttacatgac aaaggaaact2281 acgagccagc accactgaac ccacccaaac caaagccaaa tccaaacccc aagcagcctg2341 attccaccgg ggatgacttt gatttcacag atgtttcttc atggtgaacg aaacagggag2401 ttgcctcgat tctgatgtgt ttgcttcggt gctctgtgta ttgtattcat cccactcttc2461 aattcataaa ctctggagat tcttcaattc actgggcaaa ccatatgcct aagtctatcc2521 tctgatggag gctcgtacat gcaaaatcca atagcaacct gggaagattt cagtcaagac2581 ccaagaggag gctggcatca ggctctcggt caccgatctt cacattcagg tttcctgatg2641 cactttgcga ctctttgggc aacctctgga ctccttgttc ccagggtcca catttagttt2701 tatctttact gcattgcttt tatgaaaaag aataaaattg gatgaaacag<210>34<211>553<212>DNA<213>Homo sapiens<400>341 ttaaaatgac ttttatttat tacacaagtg cttagttaca cttatctgaa aattataata61 ggacaattgg tgcatgtcac attcatatta ctatccatat aagaacaaat acatttagtc121 aactatatca ggataacaca caaggttttt gtttgtttta ggcttctcag ttgaaagaaa181 acatcgagta aggaaaatga atttccagtg attaagtatt aacaatataa ataataaaaa241 ttacttctaa atgtcttaca ttttgacaac tttgaattat acttacatac taatatttcc301 aaaaatcatt taagttacaa gggtcactgt cagggtgaaa atatattttg aaaagctttt361 taaagatatg ttattattat gctttaacat catttcattt attcttggca cacacggcat421 cttggaaagt aaaaatacat cttgccaatt ttctgctatt gtgcagttac aagagaaaga481 gaagtgtggg gcagacagac ctgtgttcag ttactagctt tgtccaaaat tgtgttgagg541 ctggtcaatt acc<210>35<211>823<212>DNA<213>Homo sapiens<400>351 tttttttttt agaaaacaat ttttatttaa aaaatcataa aaagtactgg aatgaaaata
61 caggtaaagc atttgaaaat tatttttctg gcttagactg tttactggct cgattctagc121 tctgtcctgg taaaggcctc cagaaccttc acctactatt atcaagtctg gcctcatagg181 gagggacttt tactgtactg aaaggaagcc agctgaagcc gagatctgaa gcgctcagct241 tccagttccc ggaagaaaac atcatcatca ttctgtgtta tcatgtcctg cagctgctga301 atttccttct ccatcttaga tctcagctcc ctcttcacct tatgtaatgt ctgggcctga361 gatttctctc tggctttaag ttcttgatgt tcctgtgata tggcttctgc cagcaatgaa421 aactggtcct tatagtagtt ctccatggag tccagttcat cctggtggcg tctccttttg481 tcatctcgct tttctttggc atagntnctt aggtctcgta atctttgctt ttgaatgttt541 aaacctcttc aaaacagttc ttaatatcat ttcttccggg tcctcattct atcattttgc601 acacaactga ctctatatca tcatatattt cgagcacgag aattgctgcg atttctttat661 ctggatggtc aacttgccta caatgcgccc ctgagnctga agctntggta tgtatatctt721 tttgacaggg aanaagagat gctttttagc ttctcaatac aactgaattc tcttgacatt781 ccttcatgtc tatctagctg aacctgcaat ggtgtcacat ttg<210>36<211>550<212>DNA<213>Homo sapiens<400>361 tttttttttt tttttttttt ttttaaaacc ctcatcaata gagggggaca tacaaaaata61 gtcaaaccac atttacaaaa tgccagtttc aggggggggg ttcaaaacca aagggatgtt121 tggatgtaaa gggaaatatt agttggggga tgaagcaaat aggggggaaa gttgagccaa181 taatgacggg aagtccgggg aagcctgggg ctacaaaaaa tgttgagccg taaatgcccc241 cggaaatggg gaagggagac tccaantact ctgaggcttg tgggagggta aaatagacac301 cccgtaaaat tgtaataagc agggcttgaa ttatttggtt tcgggtgttt ctattaaact361 atggggagct caggggattg atactcctga ggcgagaaaa aacggatgtg tttaggagtg421 ggacttttag gggatttaaa cgggtgaatg ttgggtgggg gcaaggcccc tccaaatggg481 ggggtagggc ttggctggaa tggtaaaggc tcaaaaaaat cctggaaaaa aaaaactttg541 gggaataata<210>37<211>477<212>DNA<213>Homo sapiens<400>371 ttagtgcagt acattgttgg aatttatatt ttaagttata aggtataaca aggtgaatct61 gggaaacaga ggtgaatggt aggttactgc ctaatagtga gagtcctaga aagccacact121 aagccttctg ggatctaatc ttacttggaa ttctctatat tgaaggtacc agccccattt181 ctcctttggc ttttcagcag agtatttttg aaagaagcag gaattgactg gacagagctt241 ttgatcaaat gttaagcagc ttctgggata tttggcattt ttagtaagtt tactatttat301 ttcatcagat tctgatcaaa ttttctagta acattgagct ggattttcga acatatttat361 ctctaccgtg tgtgcgcgtg tgtggttgtg tgtgtatctt agatagctgg aaccaatttt
421 aatagccttc tgaactttac acatttaaaa cttccattaa atgaaactaa attgacg<210>38<211>531<212>DNA<213>Homo sapiens<400>381 agagtttatg aaaatataaa gggttttatt tgttggaatc caacaagata tatgcccaaa61 tatatagtca tcaaacataa aattcagggg cactggagat atatataata ttagatgatt121 ataccaatag ctagactttg aactccagtt attattaaca taaaaattac agtatgacta181 ctaaataatt agtacagtca atgtggtaaa tacattaaat tacaaatatt tacatttgaa241 catagctcat tttacaaata aatgttttaa gccccaactg tccaactttc actgaaacac301 taaatgttgc tgaacaaaaa gtctgcaata aatactccct ccagtgagtg ggggaaatga361 ttttacatac actctgtgtt acaatgaatg tctgagtata tatttaaaac tttaaactcc421 tcatgtgatt attgaaacct tgtattctgg aaaaactcat attgtaaaca aaatttagtt481 atttatttaa aaagtaaatg ttctcattat gctaatatta ttcatggtta g<210>39<211>592<212>DNA<213>Homo sapiens<400>391 acatttatag tgaatatgtt taataaaaat ttgcttcaca aaatggtcag aagactcact61 tgatatgttt gaaacatttg aaacatgttg gttacttttt tcttttcttt gtgtctgcaa121 tgttcttcat cagcgtcctc tcacttttca gctaccactg ctttaaacag cattgtccac181 agctccgtct gcagggtcag ggcatggcct ctctccgtgt tcctgtgaag agccttcatt241 ggaatcatcc cgggacatac agcttgaatg tgctgtctgg ctagcccctc cacaagtcgg301 tcactctgca caaggaatcc gagagctcat caaggatcag cacggtctgg ggcccaggtg361 gggtggaaca cgcacggtcc acaagcaatt ctgtctttct caaggctttt tcttgtgcag421 tatgaaatcc ttcatatttc atatgaagta tgtgccttct ggggcactga gctcaggaac481 tccaaaaata ccccttcggg ccggatcccg gcttcaaggc tgccccgacc cgccgcctgc541 gccgcagagc cgctgctctg gctccgaagc cgcccggctt tntggcgcac ag<210>40<211>603<212>DNA<213>Homo sapiens<400>401 agattagtaa accacagcaa agtttattca tgtgccaggg acataaagat acattaattg61 tttgtttttc taggctgcca gtataagctt ttcaaaatgt ttaaagtaaa taaaaccaat121 aatacagcta gataccacat tgcaaagaac cctaatgcta tttatcagct ttaaaatact
181 ttcaactgaa atcaattaca ttttaatata cacatattat acagaaatta cctatagctc241 ttttaaaaat tctatgacat taacatgaaa ctttattaga atttaagatt ctatatgcag301 acctgataaa gccttaaatt taattatttt ggagtaatgt cttcttaaga aatggtagat361 aggataaaat aatttacaga tttatctgcc tttgaactgg aaggaatttt aggtaatcac421 ctggttcaac ctactcccta aagtcacaca gctctataat ttccccctca ctcagctact481 tttttctgtc taaagatatg tcaaagcgat ttcatatgta atcatgagac attcaagtgc541 aagataattt accaagaaat cccactccaa gatccactgt acttatttaa caagacaaat601 taa<210>41<211>533<212>DNA<213>Homo sapiens<400>411 tttttttcag gcccgatggt ttttatttct ttcagttatg cttgcggctg gagtggggac61 gggggaagag cccatggcct gggctccctg gagaccaggg ctgatgatac cagctgccat121 aggtgaggta ccaggcaaag gtgcccactg cggcgcccac caccttgtga gtgtactggt181 ggaaatagat gacggtacag agcagcaaga agttccagag gcccagcagc agcacgttca241 gcaggaagac aaggcgcaat gggggcgccg gcaggaagcc catgggccag gtacttggcg301 aacacagctg cttcctctgc catgagcagg cagcaaaagg tgagcaggaa ggtgtgggag361 gagacggtgt agcctcgcca ctggtggccg gctgccaggc agctgcggcg gtcaggcagc421 tcgtggagca gcagaccctg nggcagtggc tcgaagcagg agccagtcag gtcctcgatg481 agcaggaagg cccggccggc tccccgccac acggctgccc ctactaccag tcg<210>42<211>489<212>DNA<213>Homo sapiens<400>421 gaggatacat atcttttaat aacaaaatgc ggggaagaga aaatatctaa atatttgggt61 gtaattattc caaacataga aaacacacaa tagaatcaag tgaagggaaa gctactgtaa121 cagcttagat actttttaag gagaactgat agtaaatctg ggaatgggaa aaaaacacag181 agctagaatt tgtttagcac tccgtgcaaa aggcaaaagc tgataaaact ggagtggacg241 gaggctccga atgtggccct ccgctgctct ctctcctgca gtctcatccc cggccttagc301 cctgacctct cctccgaagc ccctgaaggg agaagtgtgg ctaagatgga aattgctagg361 cagcagagct gttggcttgt ttgcatttat tgctttagga acccagagtc cactcttgca421 ccgggcctgc ctgcctgtga aggcagagct gggttgctca gagcacaagg cctaccccat481 cctgcgtct<210>43<211>526<212>DNA
<213>Homo sapiens<400>431 aacctgcaaa ctctttatta aattctccca tttcatctgt acagaaaaaa atgcacatta61 tgttcagaac atatctcagt aacatctcaa aattacacag catgaacatg taaaaacaag121 ggaccaccac gattttatac atagaaagga aacccattta caaaagaggc ttgttaattg181 tatttttttc tttctttcaa aaacaaaaca aaacaaaaaa aagtgaaaag cctaagatct241 cacacagcat ttgctgtaca gactgttttc cggatggact ggtttgggaa cactgtgctg301 ggggaagctg cccaggaagc gctcccgctg ccggctttcc ggaggtctct gcccagtgca361 ctgcaggggg acctggaggg cccatttcta ccaccctagc atgtctgaca gaaagccctg421 ctgggctctg gggtccagat gtcaactcta cattggagga ggcaaaacac aatctagagg481 cactgtctga actttcccct ggcccaggga gatttctcca ctgcac<210>44<211>501<212>DNA<213>Homo sapiens<400>441 ttttttatct tttttatata atgatttatt tttctttggg tgtattccca gcaggcagat61 ggctggatca aatgctagtt ctagtttcaa ttctttaaaa aaaaattcca cgcagttttc121 cagagaggtt gtttttggac tgagcaaccg agagggagag tgaccatctc ctgatggagg181 agcagcccca cgggtataga ccaggagggg gagtgaccat cttctgaggg aggagaatcc241 catgggtata gaccaaggag gggagtgacc atcttctgag ggaggagaat cccacaggta301 tagaccagga ggggagtgac catctcctga tggaggagca gccccacggg tatagaccag361 gagggagagt gaccatctcc tgatggagga gaatcccacg ggtatagacc aggaggggag421 tgaccacctc ctgattgagg agaaccccac gggtatagac caggagggga gtgactatct481 ccagatggag gagaatccca t<210>45<211>552<212>DNA<213>Homo sapiens<400>451 ttagccaaaa tgaaggtcct ttaattcaca tgactttagc actgaaaacc caaaacatat61 gatgctcttc tttcacacca aaggcattgt gatgattagc gtggattttt ctttgataaa121 ttttgctgtg gtcatttcag tatcaataag ttttggaaga ttcagatgta atctgtactt181 ctcagatact tcaatcaata aatcatcctc agaaacacta aggtcacaga gagagacaga241 attaatacca ggtaattcaa ctttcaactc aattttcaga ggtttctcac tgtgatcatg301 cacaattttt agttcatagg ctggcatctt catctccacc tggatttcag tactggaaat361 ctcttctatc agacacactg ctttgcctga aacttggtct tttggcagta acagttgagg421 aaagtgatct ggattgctca tagtactgct tcgtatctgt ccaagagtta gttcccttct481 cattttttct cttaaatcta tggaatcagt ttggattccc atcagatttt gtttcattct
541 ttgaatgctt cc<210>46<211>554<212>DNA<213>Homo sapiens<400>461 aaagattctc cagtacgttt atttcacaga cttttttgta cagttgaccc ttacacacgg61 gtttgaactg ggtgggtaca cttacgcgca tacttttttc aatcaaatgc aggtgaggtc121 acacagatca gcatttgtgc atgggatgtg aaactcatgt gtacggaggg tcaactggag181 ggccaacttt tctacatatg ggttctgcaa gactttctgt gggacttgag tatgggcaga241 tttttgtttt atgtgtggcc ctggaaccag tccctagcgt atacaaggga tgactgtatt301 ttaatggttt atagtgttaa aaagagaaaa atatccacat aaacacaatt ttgaacactg361 aactatttta atatgaatta atgtcttatg tttattacca attgatcttc aaattgcagc421 agcccatgag tttggttgta agtgttcttg ccattccacc ccagctgtca ctgagtcagt481 ttaaaataca cacacacaca cacacacaca cacagacaca agccttttgt aaagtaaaca541 aatcttttat actg<210>47<211>508<212>DNA<213>Homo sapiens<400>471 tttttttcca tttttttctg ataatttctg ggcacattat gggtattgtt gtatttagct61 ttacaacttc aactgaacaa cttattctag acatctttat agacagtatt cttttaatgt121 acaatgaatt atttaatcct gttattattg ggtatttagg tggttcccat tttcacattt181 aagacattgt tgcagggaat atccttatcc atccaccctt tataattaac cagttatttc241 tgaagaataa attgacatca aaagtataag acttgcatca aattgtatct ccctccacac301 catcttgaaa atacccactt tataaatctt tttttctttt aactttcaac ctgatcagcc361 agatatattt gtggtgattt ccccctaaat gactaggaaa gttcagcatc ttcatgttac421 tggacattta aaaaaatcaa ctttattggt ccaattttct aagtcaggtc tcctggaagc481 agcatttcaa attaggattc ttgtgtaa
權(quán)利要求
1.一類在人類肝臟中表達(dá)的相關(guān)基因，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.47所示的序列；(b)SEQ ID No.1～SEQ ID No.47所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.47所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
2.如權(quán)利要求1所述的相關(guān)基因，其特征在于，所述序列包括具有SEQ ID No.1～SEQ ID No.47所示的序列。
3.一種探針?lè)肿?，其特征在于，所述的探針?lè)肿雍袡?quán)利要求1中所述的序列中約8-100個(gè)連續(xù)的核苷酸。
全文摘要
本發(fā)明公開(kāi)了一類在人類肝臟中表達(dá)的相關(guān)基因序列，它包括SEQ ID No.1～SEQ ID No.47所示的序列；SEQ ID No.1～SEQ ID No.47所示的序列中每條序列的互補(bǔ)序列；與SEQ ID No.1～SEQ ID No.47所示的序列中每條序列有至少70％同源性的序列；上述三種序列中一條或數(shù)條的組合。利用本發(fā)明的人類肝臟相關(guān)基因，可為研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
文檔編號(hào)C12Q1/68GK1970759SQ20051011059
公開(kāi)日2007年5月30日 申請(qǐng)日期2005年11月22日 優(yōu)先權(quán)日2005年11月22日
發(fā)明者黃健, 韓澤廣 申請(qǐng)人:上海人類基因組研究中心