專利名稱：人類肝臟相關(guān)基因的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物技術(shù)領(lǐng)域，尤其涉及一類在人類肝臟中表達(dá)的相關(guān)基因。
背景技術(shù)：
肝臟是人體的第一大器官，是物質(zhì)代謝、能量轉(zhuǎn)換及供應(yīng)的“樞紐”，是機(jī)體內(nèi)多種重要信息調(diào)控分子的“集散地”，是機(jī)體內(nèi)再生能力最強(qiáng)的器官，在人類生命活動(dòng)中占有重要地位。肝臟的主要功能是進(jìn)行糖的分解、貯存糖原；參與蛋白質(zhì)、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機(jī)能；制造凝血因子；調(diào)節(jié)血容量及水電解質(zhì)平衡；產(chǎn)生熱量等。在胚胎時(shí)期肝臟還有造血功能。
肝臟是淋巴細(xì)胞以外最常見(jiàn)的病原體持續(xù)感染的場(chǎng)所，肝臟疾病又分為肝炎、肝硬化、脂肪肝、肝癌等?，F(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)證明，肝病病毒侵入人體后，并不直接引起肝細(xì)胞的損害，只是在肝細(xì)胞內(nèi)吸收營(yíng)養(yǎng)賴以生存，并在肝細(xì)胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細(xì)胞膜上，引起人體免疫系統(tǒng)對(duì)這些抗原物質(zhì)產(chǎn)生免疫反應(yīng)，這種反應(yīng)造成肝細(xì)胞的損傷、壞死。免疫反應(yīng)的強(qiáng)弱決定于肝臟受損程度及臨床癥狀輕重。這場(chǎng)由病毒引發(fā)的、免疫系統(tǒng)對(duì)肝細(xì)胞的戰(zhàn)爭(zhēng)，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場(chǎng)，肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身，它還可以引起其它多種疾病。常見(jiàn)的有(1)糖尿病；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎?。?6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結(jié)節(jié)性動(dòng)脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對(duì)患者的身體甚至生命造成危害，而且對(duì)患者心理上的打擊也是十分沉重的。無(wú)論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學(xué)等方面都會(huì)受到嚴(yán)重影響。
肝臟疾病相關(guān)基因在以肝炎、肝癌為代表的重大肝病的控制、診斷、防治與新藥研制領(lǐng)域起著重要的作用。
在本發(fā)明之前，還沒(méi)有出現(xiàn)涉及本發(fā)明的一類人類肝臟相關(guān)基因的公開(kāi)報(bào)道。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問(wèn)題是提供一類在人類肝臟中表達(dá)的相關(guān)基因。
為了解決上述技術(shù)問(wèn)題，本發(fā)明通過(guò)如下技術(shù)方案實(shí)現(xiàn)在本發(fā)明的一個(gè)方面，提供了一類在人類肝臟中表達(dá)的相關(guān)基因，其包括(a)SEQ ID No.1～SEQ ID No.35所示的序列；(b)SEQ ID No.1～SEQ ID No.35所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.35所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.35所示的序列。
本發(fā)明還提供了一種探針?lè)肿?，所述的探針?lè)肿雍猩鲜鲂蛄兄屑s8-100個(gè)連續(xù)的核苷酸。
由本發(fā)明的一類在人類肝臟中表達(dá)的相關(guān)基因，可為研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
具體實(shí)施例方式
下面結(jié)合具體實(shí)施例，進(jìn)一步闡述本發(fā)明。應(yīng)理解，這些實(shí)施例僅用于說(shuō)明本發(fā)明而不是限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來(lái)源于5個(gè)成年男性，在肝臟切除手術(shù)后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內(nèi)，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實(shí)施例2cDNA文庫(kù)的構(gòu)建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補(bǔ)平末端后，加含EcoRI切點(diǎn)的接頭。磷酸化EcoRI末端后，用XhoI限制性內(nèi)切酶消化1.5小時(shí)，再進(jìn)行片斷分離。過(guò)柱篩選長(zhǎng)度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無(wú)菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進(jìn)行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細(xì)菌。涂板并測(cè)定滴度。
實(shí)施例3測(cè)序及數(shù)據(jù)庫(kù)建立(Seqencing and Database Constructing)挑選文庫(kù)中有外源片段插入的克隆，擴(kuò)增后抽提質(zhì)粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標(biāo)記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測(cè)序儀(Perkin-Elmer，USA)上進(jìn)行EST大規(guī)模測(cè)序。測(cè)序結(jié)果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450 Server上進(jìn)行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(kù)(Genebank+EMBL)，將無(wú)同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫(kù)。
實(shí)施例4基因的全長(zhǎng)克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上，進(jìn)行cDNA全長(zhǎng)克隆，分兩階段進(jìn)行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針?biāo)褜bEST數(shù)據(jù)庫(kù)，將重疊序列＞50bp，同源性在98％以上的表達(dá)序列標(biāo)簽(Expressed Sequence Tag，簡(jiǎn)稱“EST”)序列認(rèn)為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進(jìn)行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開(kāi)放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長(zhǎng)完整性如何。通過(guò)電子克隆的方法，通?？色@取人肝臟相關(guān)基因的全長(zhǎng)序列。
(2)cDNA末端快速擴(kuò)增(Rapid Ampiification of cDNA Ends，RACE)如果通過(guò)“電子克隆”方法仍未得到完整的cDNA全長(zhǎng)，則在已有序列5’或3’端設(shè)計(jì)引物，在人類肝臟Marathon-Ready cDNA文庫(kù)(Clontech Lab，Inc，USA)中進(jìn)行長(zhǎng)距離PCR反應(yīng)。然后對(duì)PCR產(chǎn)物克隆、測(cè)序。用AUTOASSEMBLER及STRIDER軟件分析被延長(zhǎng)的序列有無(wú)完整的ORF，如無(wú)，重復(fù)上述過(guò)程直至獲得全長(zhǎng)。
(3)RT-PCR對(duì)于5’和3’端的已知的序列，如果中間有一段間隙(gap)無(wú)法從已有的公共數(shù)據(jù)庫(kù)或自身數(shù)據(jù)庫(kù)獲得，可考慮采用RT-PCR的方法。在序列5’端設(shè)計(jì)引物，3’端引物采用Oligo-dT，在肝臟總RNA庫(kù)中進(jìn)行擴(kuò)增。然后對(duì)產(chǎn)物進(jìn)行克隆、測(cè)序。最后拼接便獲得全長(zhǎng)。
通過(guò)組合使用上述3種方法，可獲得人肝臟相關(guān)蛋白的全長(zhǎng)編碼序列。
序列表<110>上海人類基因組研究中心<120>人類肝臟相關(guān)基因<130>NP-10163<160>35<210>1<211>646<212>DNA<213>Homo sapiens<400>11 tttttttttt ttttttttga gctaaaggat attttatttt atgttcctgc atacttttgc61 caaatggtta catacaaagg atgaagcctt tacattccca ctggcagaaa atgagtaccc121 acaagtgccc caagtctatg gtaacctgtc ctatggaggg aaatctgaaa gctcttagtg181 agaccagtgg gcagatgctg agcctgcccc cagacagagg gagaagtcga accaccaagc241 atttgctagg cggcgcagca tctatgtgat cagggccaat gatattcttt tgctctttcc301 agcattgccc agtgatcctg gggatgccgt ttggaagctg cacacctttg acatctttag361 ggtcgaagag atgatgctcc ccatggggac tgaccagtga tccattagtt tcttgtggag421 tcttcggagg tctacggggc cttcctgggg tgcacctcat ttcccccact cctgacttcg481 caatttactc ctttggatct ttccagaaac cgtcttttgc agttctgaaa caaaggccac541 ctttcctggg gtatttgtat ggggcagnca cccttttcac atgcttctgg agttcccgcg601 ttagtgcctt tggtcatgaa gagagtangc tgagaaggac tataat<210>2<211>564<212>DNA<213>Homo sapiens<400>21 caacaaagca tatttttcaa ccggtttatt gtatctttac aataataaaa ataaattagt61 cacacacaaa aatagtcatt tttgctgaag agaaaacaga ccatatttac tcacataatt121 cgccttctac ctttcacctg cttatgtaat aatttaacac tgtagagggg acatggaggt181 gacggagtat ttagtgggtt ccttgctcct ggctgtgcag ttcacaggcc ccacaggcct241 tggccccagc attccccaag ctgggacagt ggaagtgtca gagcagtaaa tgccaccact301 tccctctcag cctgtccctg tccccgccag ccacctgcct cacccacggc tctaaccaag361 cccaccgagc aactgacgct gccagtctaa caacggggac aaagcaaaga gaaagggata421 acagacaccc tcccatttcc ttctccaaat gtctgtggat gaggggaagg cagattagat481 gaggaaatat aatatttaag atgaaaaagt agcaatcagg ttcaggtaca atgctacctg
541 aatgagtcga aaagcagagt aatt<210>3<211>524<212>DNA<213>Homo sapiens<400>31 tttttttttt tttttttaca gaggaggagc tctttattgt gggaggtgtc tggaactgca61 ggaaggtcag cagcgcagtg gggcgcgacc ccagccagct ggcagttagg gctgggctgg121 gcgccatccg ccccgtttcc catctccctc ggcgcatccc tggggagccg ccatcactcg181 gcagattccg catcaaacct aaggtcctgg agaacctcgc tgatcgcctc catggtttta241 attaccttca ttttcagctg ctgcatccgt acagcgtcct cctgccccgc cagctctggc301 gtctccatca actgcagcag ggccaccgtg cccagccgcg tctgctccag ctcctgcagg361 ctgcgcacgt ggccggcgag cagcggctgc ctgcgaggcc cgcccagctc tgcttccacc421 gccagcacct cccgcgaggc ggcggcgaag gcctgggtca ccccgtgcac tgtgctccgg481 tagcgcgcga agtcgtagtc agggccgctg cgcaggtact ggcg<210>4<211>520<212>DNA<213>Homo sapiens<400>41 tttcatcaat tcattcatat ttcaacatac acttgttgat gcccggctct gtgcccgaca61 agatggtagc tgtggagact gatctgaaaa agcaaccttc gtgacaggtg gaagttggat121 gggaacaacc cacccagcca attaggttcc tcagttgcat gacaccaagg agctgctgtc181 tgggaaatgc acttattacg agagggacaa agtgtgactg agccacaggc tcacctcagc241 cagtcaaaag gacttgggca ctgtctcatt taagccacct gggagcagac gtggctacgc301 tcccagcagc cctgtaccca tcggccactg aagatctcag ctctcgtcag ccttgcaaag361 cttgctgcat ctgagtcttg ggctcccggt ccactgctta aaaagttact atcacacaaa421 gtcgaaagta gacagttcct cttgacctgg aattgtccaa gtcagcacca cagctggtta481 aggcaggccc tctctctttc ctcttcttat tntcttggct<210>5<211>771<212>DNA<213>Homo sapiens<400>51 tttaaagatt aactaacatt ttattaattc tgattccttt tatcatgtgc ttttttatac61 aaagcacttt caaatacatt acattatctt aaatttataa taggagtttc tttcggattc121 agtttaaaaa tgacaaatag cattcgttgt gcccaagtta gaattacacc aaaattacca181 tgtgctggca cataccatca tcccactggt ggctggaaaa ctgggttgca ggagtgtctg
241 tcactgagat gggccaccac cccagttggc cattatggta gagatgaggg aaggatggac301 tagaagcaag ctgggtcttc tgggtcgtct ctactccttt ttcacttcat caccgttttc361 cccactgagc ttgaacacag gaatctgctg accatccttg agctctaaaa agacctcgtc421 aaggtgccac ctctgaaagg ggccacctct ggatgagtgg acgtagagaa ggccctctga481 tttggatcca gagacaggaa tcttcaactt ggcatcaaca atgtccacga agtttttcct541 gtcgatgagc ttgagataat ggatgntgag aggagggccc agagcttcct gtgccttcgg601 atggctctgc agctgctcca ctgccaactt gttatattaa gccctggatt ganacttttg661 aatgaggtaa tacacantgg caaagncccc ggcattagaa cacaccgtgt gaaaaagatc721 cttgctcccc agagcatttg accgcctgct tcctgcatac ttttgccaca t<210>6<211>596<212>DNA<213>Homo sapiens<400>61 tttttctgga cactaaatgt ttaatattag aagaatgatt acacatagct tgttacagat61 ttccaaaaaa cagtaggtac agtttttaaa atttacattc acagagaatt ttaatgacat121 tggaaaatgt aagaaacttt gaaaaaagat ggagtaaaat atgtataaaa ttgataatag181 ttgatttagg gtggtagaag taaacataat tttttctgtt tatatttttc tctatctttt241 aaattttgct aatgtgcata gattctttta aaataataag aaaataataa agttaatacg301 ttataaaaaa tagggacctg gctgttgaag tgcgatggag acaatttgtt agaacatgtg361 gcttgttaca cagacgcttg agaagtttgt tgagagagaa cgattaccta gaaacaagag421 ttacagtaaa tggggtaaaa agggcaaaag ttcttcagat tactatccta tttaccaaag481 tttgtgatat gtattttgaa tatatgtgaa gagcttcact tctatcaagc catagcactt541 atttgtcact ctgatataac aatntaacat aaaattgagt tcattcaaat gagcat<210>7<211>573<212>DNA<213>Homo sapiens<400>71 tttaacttga acaggtaatg tttaatatag agaattactg gctttaacag ttaactggaa61 taatgagggc ttcactggta aaatgcttct gaattgactg gaaatccatt tggggtgctg121 gggaacgtta ttcccagaga ggtgcctcag tggaggcgct gtgtctccta cgcaacttct181 gagggctgga gggtgccaag ggcagctgct gaccgcctgg tgcttcagga gctgggtgct241 ggggaagcca catgcactgc ggcgttcaga ggcagaagca caaccaacaa gaaccacgaa301 ggaggcgcct ttcctcctat aatgcctgtt tggtgccctc tactgacaaa gcttatcccc361 tttcaaaaaa cagccaactg aaaaagctga atttggaaca taaagtcaat aaatccataa421 ccagcaatac tatggggcct ggggtgcgct ggcctttagt gagtggagtg gggcgaagga481 tgctgcatgt cctgcagtgg gcacagcggc cctgcacggg ggagaaccat ccctgtaaag541 tgtcagtagt agcctctgtg tcagtcaggg tcc
<210>8<211>591<212>DNA<213>Homo sapiens<400>81 tttttttagc attaaatctt ttattttgaa acatttttag gtttacagga gagttgcaga61 aatagtagag agatttccca catatctttc atccagctta cataaccatg gtacatttat121 caaaacacag aaattaacat tggtataata ctattaacca aactacagac attatttgaa181 ttttaccagt ttttccacta atatcttttt tcttttccag ggctaagtcc aggatagtac241 attgcattta gagctgtatc attgctttaa aattcttcac agattcacaa tccctcattg301 cctgccctgg gggctgacca ctcccagctc ccctactcca cctgccttgg tatacccctg361 attccactaa acttttgtgg tattgggggt aagaagatct aatggcttca agttagcaat421 ggtgtgttaa aatttttttt tttaataaaa acaaaataaa agaagatcta atggttgtga481 agtctcatcc tttggtgaaa ggtgctgttg agcctgatac aatatattca actgccagag541 cacagacact tctactttgt ctagctggag attctttagc caggtactcc t<210>9<211>593<212>DNA<213>Homo sapiens<400>91 tttttttttt tttttttttt tttttttttt taaaagggtc ctccatgggg cggttaatga61 agtatatgca actagtttaa cagcatgaca attgttattc caagacatct tcagtaactt121 ttgaaatagc aaatttaaat tttaacatgt tcttatattt aacatgtttg atattttctt181 ctaaaatatc atagcaaata aatattcaac atacaccaaa agtacttaaa agaagctcct241 ttcctttgga catcaacttt taaaaacacg aacaactttt tgaaagacag aatttacaaa301 tacagaactg tactgactta aatttggaat ttactaatta ctggggatac tttagtgagt361 ctgcatatgt gtattattaa tacatgttaa accatactgc agataacaaa aaatatactt421 acatttctct tccagagagt aatgactgta ttcaaagtct gagggaatga caaaacggga481 tgcacatcta acactgatac acggttcttc agaaaagact agtttcagct gtttccaggt541 ttacataaga tgatggaagc agtctctaat atgttaatca agaaaatata tgc<210>10<211>565<212>DNA<213>Homo sapiens<400>101 gttatttata aaataattat ttattatgct caatatacat atttcctttc agatgttaac61 atattgtttg atagccatca ttaacacagt acttagcact aaaattgtac ataataggag121 ccactgcctc acagccagcc cacaaatttc ctacagtttt cctgtcgatc agttatttcc181 tttgattcat tctgtacatt cctgaaaaac atctgtatgg ttttccagta aaactgcatg
241 agtaatatcc atgcttttgc acttaaacgt caatttagat agacatgcaa tttgtgctgt301 agtaagtgac ctaattctcc taccttacaa atttccttca cacaaaaaac agtctgatgc361 tttttagttt ttatcttttt gttcccctga acatagtgag tactgcccac ttctggtgta421 gtatcttgag tttagggaag tttttgtcct gtttcctcta aaatgttgtt cctgttacat481 gtgttctgtt agtattttcc ttctatcact taacaagact ctggattctc attgtctgat541 ctctgtatct atgatattct gtttg<210>11<211>548<212>DNA<213>Homo sapiens<400>111 ttttttcgca catcaaaaat ttatttcttg ctcatattac atgtgcatcg cggtcggcag61 aggggctctg ctcatcacag gctatcaagg atgacagcag acccctccct tctgccgcag121 ttacacttac aggtggactc aaggttgaat gtcaaaggca gaaagttaca acctgggaat181 acaggataat gcctttgtac cccccaggta gggaaagatt tcttaagtga gaaacagcat241 cccatacagc acaactgggc agtgcctact caacagccaa ggcttggcca tccccactcc301 tcagtatggg ccccagaacc cagggcctgg gggactctgc acacaggcat gtggagacca361 cagaagcctg cccaatatag cgctgccagg aacagcaaag acaaaaccag agaagcctat421 cgcgggcacc gctgtgggca gaactatgtc ccttcaatat ttttatgttc aagtcctaac481 ccctagtact tacatttgaa gacagggtct ttaaagaggt aattcagggt taaatgaggt541 catgaggg<210>12<211>715<212>DNA<213>Homo sapiens<400>121 tttttttttt tatcataaaa acctttttat atcagctcac ccacacctcc ctccgttcct61 ccctgccctt tctcgccctg gtgatcatca gggatgctgg agtctggcgc ccccccacac121 caccagagct gaagccgaca ttcaaggtgg acgtggcgcg tggagagcaa gggaaggccc181 agctcctctc cttcgtcccc actctctgtg ggctcaggaa acacacgctg accggaggca241 gtggccagga gaggcaggct agggtgcagg ctcacgccat tggtgcagtc cttctggggc301 agaaaactca acacgggctc cggcttccca tcattgccag gcccgtccgt gtcccacaca361 gagacagccc cgctcgtgct gccactcact aggaactgcc cggtcgggtc cagatcgaag421 tagatgcgct gattggtggt cacctctcga cccagggacc acagtgggta accagactgc481 cggagatccc agcacaggag ctcagcatcc ttgcgggctc ctgagaagaa gcggttgcca541 tcgggatgaa agcagaggtg gggatgcccc cttggtgccc tcccagcaag gcgagagggg601 agccatcatc ccaggcatac agacccaggg agccgccgta ggagccacag gcctagaggg661 gctgggctgg gctgaaagct ttncaagaaa tgatgcggtc tggccctgct ttttt<210>13
<211>551<212>DNA<213>Homo sapiens<400>131 ttacatgcat aggcatttaa tcaaggtaag aggaacagca gtggaactta aatatgatac61 aatttatcaa caataaataa acatttcagt gcaaatagtg cagaaaaatt tctcaaagat121 catagcaatc attctaatcg gtacaaaatc agtctcagtt tctgtataca atctatatag181 gatattctng tgaatttagc cactgatgaa agcccagttt gaaacataat taaacaggnt241 ttggtttttt ggtcccattc ctttgggcag tnccatgttt cagggaaatc tagaaagaaa301 catanaaata ccttaatttt aagagaggaa atggtatctt ttacatttct ttcattagat361 tgcccccagg ggctcacgca gatgctgaag gacagtttcc agctcagtcc cagagacacn421 gaaggggggg ggatttttga tttcagtgga accgggttaa tcatggaatg ttaaaacngg481 gggtgaccac gggggggaac aaaagnnggg gggggttgct cccggtaggg ggggggttgg541 gaaatactcc t<210>14<211>516<212>DNA<213>Homo sapiens<400>141 catcaaaagt ttattgagca tccagtatgt gctaggcact ctgctggatg ctagtaatac61 aaagatgaag atgaaaacaa cacaaaaaca aaccccactt ccttacttac ccctccccac121 ccccaattta aggcagattt ggcttaagcc tggcaagctg gcccctcagg gattcagcac181 cagggtatgg catgttctca tctcacccta accttcacct gtgcatctca aggctgacca241 gcaggtactc cttatccaag tcctgctgcc tcttccacct tcttgagaaa cttttcccct301 acatgcatta tctcattatg gatgaggcac ctgggaagtt taggggagct gcgaaggcac361 acctgctcaa atgagcctgg ggaagtgccg agggacaggg agctgagaca ggcatgctgt421 ggggttcagg gcanaggggg caaactagaa atgtcagtgc ctttggggct gagacaaaac481 ttgacctgnt gtggaggtga tgggtaactg gggcct<210>15<211>586<212>DNA<213>Homo sapiens<400>151 tttttttttt tttgttgttt cccttcattt actcacagat gaaaagatta acacgtcagc61 aaatcaaaat cagagaattc ctaaggcact ctgtgcaaca ggagcgggtg gacgtggggg121 tgggtgcacg actggcgcgg ctgcctccac acgggccctc ctgaagttac cttggcccgg181 ctgctggcta cgggaacgct gggtcctcga tgccatggtc ggggttgcag ctgaaggcga241 tggtgatggc gtaacgggtg ccccagtgga ccttctccac gcggtgtagg ttctcggacc301 ccgaggtgaa gaaggagacg cgaccagctc tcggctccac cgtcttgttg gcaccctcct
361 ccatgaacat gaaccgccct ccgccgaagt cctccaggta gttggagagg tacagcagcg421 aggtgtagtc gaaggagccg taggtcacct tgtccacgtg cgcatgccag tactcgtcgt481 gcgccgtccg cgcttccgtg ctgtttatgc gggagaagaa ggtgggcttg gtcagatgca541 gcgaggatgc gctgatgcca aaagcctcag caatggtgag ctggac<210>16<211>620<212>DNA<213>Homo sapiens<400>161 tttatttata acatttcctt tatttatcaa cagcaacgat atgacattac aaagtgaccc61 gtgacatctt ttttaatctt gcacacgcac tgcagcaagg ttaatcctgt atgtgaaaac121 ctctgagtga ctatctcaaa gtgtatatgt catataacct gctgagtttt ctgatattgt181 aatactagat acaagatgtc tgaataactc cttacattta taaattctta tttaatttta241 tagctttaat gcggtccatc atttctaata atgatattgg ctacaagaat aatgatcata301 ttatttatca tataatttac cattttgtaa gcaacaaatt gcccattaac aatgaaattg361 tagttacagc atctaaaatt attagaaaat tatattagca tggaaatatg ttcatgatat421 atcactgagt gaaaaaaatc tggctaccaa atatgtagtc tgactcattc ttatttcaaa481 aaaaaaaaat atgtgtatgt ttgaaaatat gcacattagt atttgtattt tatatatatt541 atgtacatca ttatatatat atgcatacat atttgtatat acacacagat agcacaggag601 agagacagag tgggagagag<210>17<211>468<212>DNA<213>Homo sapiens<400>171 atcaataaaa tatttttaat gcctacaatt tatgtataca agaaattgta aaactttcac61 ctgtatatgc aaaagtatat tctcttgcag gtcaacagca ccagaaaaca agctaagcta121 tctaaaaatt tttttaactt gatttgtcca ttgtgtaaaa gacaggatgg atttaattac181 tccaaagaaa tctctatctc actcttaatc atacactaaa cgtctgaata tttaatcaca241 tccactcaga aaataaatag tagtctaata tattcaatac attgaaaata aaacaaccag301 tctctcttca tatatttatt ctctttccaa acatgttttg gtctcctgct atatctcaga361 aatttaccaa tagctgcttt taagttacaa aatacaaaac aacaatttat ttcttggaga421 aaatcttggg gattacatta aaaatgaaaa attgagttcc cacattct<210>18<211>505<212>DNA<213>Homo sapiens<400>18
1 tttttttttt tttttttttt attttcacat tggttaattt tatgtacatt tcacatatca61 taaatacaca caatacaaaa agctatacaa aaaccctact aattcaacat ttaaaaaagg121 gagagggaga tactgcattt gatttctgac acttttctgt ttaaataata cttaaattca181 gttttcaaga agacaacaaa ttgaaaatca ttaatttttc agagggcttt gtgttcacat241 aaattcatca ttcaggctgg gcatgggggc tcacacctgt aatcccagca agttgggagg301 ctgaggcagg tggatcacct gaggtcagga gttcgagacc agcctggcca acatggcaaa361 accccatctc taaataaaaa caaaacaaaa cataaataaa ttcatcattc aagtatattt421 ttattgaagc aataaaccac ttcactagct gtagagtcaa actgggtaag cctcaatatt481 gagcaaaagt agagaactct tgctt<210>19<211>842<212>DNA<213>Homo sapiens<400>191 gggggttatg gtattaattc attaaaggat cggcaaccac cattgtatcc atttctggat61 cctttccaaa atatccaaat cctacactta cagaaacaca acagacctaa ggaaatgaag121 cagcttcagg ctgcgaggat gaagacacca ttgctgaagc aaaggtctcc tcctagaggg181 atagccagga tccatcacag taaacccttt tacccccatt ttataaacat ccaattatta241 actgttcctg ctgggttgga gtgtaagatg agaccaaatg gagagatgtc tagtagaagc301 cccactggng gtaacctgct taacattaaa aaaaagacag gctcagttct attccctgga361 cccacatctg taggaattat attatacagc accagtctta aatggtgttc tgcttcagat421 tcatgcatac ctgaatgggt tgaatgacaa tactgtacaa gtcaataaac tgtatcacaa481 tacaacttac acagttntgt aaggttgaat aaatactttc tgtgccagtt aaaaccatca541 gcaganactg aagaaacana ctgacattng ctactacatt aatcaatgan nagtgcacca601 tctgaacttg atntgcccat gacgttgaaa cacacagaat aacactgcac atcactgaaa661 cttctcttcc atcactactc tactacttga tataagtctg atactagtgt tatgtcaaga721 tgtcatatcg agctattaat agagcaaatc acatttaggc ataaagagct attagcacta781 taagtctatc tagactgaag tgcttgacac tatagaattt atataagaat ctttatctac841 ga<210>20<211>554<212>DNA<213>Homo sapiens<400>201 tttgttcatt ctaaatttat tgagctgcaa ctgtgtgctc aggaatgaga atatagcagt61 gagcaagatg tgaacaagat ctctgccctc gtgagcttac aatctagcag cgcagccagc121 ctattacagc cataatttta caattgtaat aaaaactctg gaaaatgcat ggtgctaact181 gatttatcag aaagtcttga cccaccaaga aatcagaaaa gacctccctg aggaaggaac241 atttaggatc agctaggtgg aaagtgaaag gaagagtgtt gcaggcagag aggacagtgt301 gtgagggtct gaggcaagac atgtaatgaa ctgaaagcca gtgtgtctag gccatagaga
361 acaaagaggg gtgttgcgag atgagcctga tttatacaag atctttgaat ttaggacttt421 attccaaaag cagttggaag ccactgaaga agctaaggaa agttcgatgt ttaggtttgt481 ttttcatagc tgactctcat tgcaccatgg aagtcagaag tggaaatggg agaacaggtt541 aggaagtttc cgta<210>21<211>604<212>DNA<213>Homo sapiens<400>211 tttttttata ttttaagtat ttatttttaa tgcacatatg ggacctacaa aatttacaga61 aaataaaaca aagcagaata tatagaatac cctcttaaga cttcttagga gctgagggtt121 ttattgctgc agttcaaaga atcaaatttt atacaagtga aagctaagat gaacacattt181 aagttaaatg gcagccttgt taaaaagctt ttttatcctg ttattgaatt ttcagcttta241 tgttaaatga aatttaaaag attgctcatg aaataattta aaccttttca aaatctaata301 aacaggtaaa aggcacctcc agtactttaa aatatttaca gcaatcccaa tagtttaatt361 ttaagggcta ttatagtaca tgcggctatt atgcatacac agtatgtcat taacactctc421 taaacagtga gaattgtaga ctctagtttc tggcaccact cccaagttat tctggtgaag481 gcataggatg accaattaca cctgtgcaac actagtgtca cagcctgtct accacagcgt541 anaatccagt tatctaaaac aatgactatc aaattcccag acaatacata atttatcagt601 agac<210>22<211>548<212>DNA<213>Homo sapiens<400>221 ttttttttta ggtcttggag ttcttttata caggattcaa aactaaaatt ccacaacaca61 cttaactgta gttctaggac atatgtatgg tcaggacaac agagcagaag ttgataatgc121 tccgaggaat caggtaagta aacggttgtc ctgagaatga gatcactcca ccccaaggag181 aaagatcgga acctttaaac tttgggttat taaaattaag cttattagta ccttgatgca241 agttagagtt tcttacaatg actctaaaag tagagccagg gtcctggcaa gtaaatgaac301 aagcttttaa tacagcttag ttatgtaaaa tactgtacac tgtaaaagat ctagaaaact361 agagcttcaa gtcatgtttt aaaaaagcca ttaagcttaa ttgaaaaacc aagtatagag421 tacaaaactg caacaagagc cacagtgatt ccatggcatc cagaccccga gtccttcatg481 cagagctgca gccggtcagt ggaagtatca cattggtgtg ctgtgtggac acaagcatgt541 ctagcctg<210>23<211>516<212>DNA<213>Homo sapiens
<400>231 ttggcaatca caccgtttat tcgcacttgg cagcaagaca atggatgatg tgggaggtgc61 caggcccctg ggttggcact aatttggagt atggttgaga cagggctgga gagaggcatc121 ttagaggtgg cccccaaatc cgcaatcggg agaaaaaggc aagaatcgac tagagattgt181 caggataaag ggaggcactg cccacccctg ctatgtctgt ctgcccccac aggggcttct241 ttaatacctg nggttccctg ngtgatgaat gtcctcctac cctggcaagg ggccatacct301 gtcgccgtgg ccccataggc aaccctgatg aaagagtatc atttccaagg gggctttggt361 ttctgggtgg ccccacctcg tcgtggggta gatggcactg aaatgctaac tgagcgagcc421 ctggaagtca ccagggggct ccggaaggtc acccctgacg tggaaggcct gagatcctga481 ggcccatttg tgtggngggc actggtggct ggatga<210>24<211>511<212>DNA<213>Homo sapiens<400>241 ttcttttttt attcaaaaaa gttttactta attgctgaaa taaaaaataa cattaaaata61 atttctacat atagttacaa agcatggtgg agcttgaaaa agcctcttgt aattttgaac121 agatgaacat gatgtctcca tgatgttcta acaatatgga aaagttaaaa ccaaaagact181 tctaaccctt ggtaagacca gagggattaa aagaccccac tatggacata ttcactttag241 tcaaccactt ttatggaatg aacacttgtg agagactggg tgtgagctga tctggttcca301 agtgatctgg aagctgaggt actactttca ggatgaatgg tccggggata ttttgctgca361 gttgcttgag gaatggtttg cactgtgact ggatgatgtt tttccacaac aactgagctc421 atgggaggag aaactcccat tatagctaaa ctgctgctaa ttctatttgt tgaagcaaaa481 tcacatcttc ctgtgatccg ggctgtaata g<210>25<211>454<212>DNA<213>Homo sapiens<400>251 aaaaaatgta taatttatta tatatgtatg tattccttaa caatatgttg tataacccaa61 caatgaaata tacatcaatg aaaatgacag ctataaaaca ataaaacttg tgaattctag121 agacatgttg agtgaaaaaa accaaatcct tagactacat agagtatgat acaatttttt181 tgaagttcaa aaagaaagaa aactataaaa taagtcactt tagggataca tatgtatttt241 aggtctttct acattcgaac aaatctatta aaaagagagc gngggggtcg gcaaagatag301 gagaagagct caaatagata aaatacaagc actcataatg ttattcttaa gttaggtgat361 gagttattat gcttgataat ttatttgcat tccccattta cccttttata agcatcaaat421 attacattta aatataagtc ccaaattatg tata<210>26
<211>2934<212>DNA<213>Homo sapiens<400>261 aaattatgca agctccagga agcactgaag accatctcag aaagttcttc ctgtccaagc61 tgtggtcaaa catgtcacat gagtggtaaa cttacaaatg tgcctgcctg cgttctgatc121 acccctggag actccaaagc tgtgcttcct cccacactgc cacagccagc cagccatttt181 cctcctcctc ctccacctcc acctctgcca cctcctccac caccactagc acctgtgttg241 ctcagaaaac ccagtctcgc taaagcactt caggctggac cattaaaaaa agatggaccc301 atgcagataa cagttaaaga tctgctgact gtaaaattaa agaagacaca gagtttagat361 gaaaagagga agcttatacc atcgccgaaa gcacggaatc cactagttac cgtctctgac421 ttgcagcatg ttaccctgaa acctaactcc aaagtgttat cgactcgagt tacaaacgtc481 ttaatcactc ctggaaaaag tcagatggat ctgcggaaac tgcttagaaa agtcgatgta541 gagaggagcc caggtggaac ccctcttacc aataaggaaa atatggaaac aggaacagga601 ctgactccag tgatgacgca ggccttaagg agaaagtttc agctggctca ccctagaagc661 ccaactccaa ctctgccact ttctacaagc agctttgatg aacaaaactg atgccaactc721 tgcctcactc catgagatga tccataacaa atacagataa aaacacccag ctgggtgcag781 tggctcacac ctgtgatccc agcactttgg gaggctgagg caggtggatc acctgaggtc841 aggagtttga gaccagcctg gccaacatgg tgaaactcct tccccactaa aaatacagaa901 attggctggg catagtggcg ggtgcctgta atcccagcta tgcgggaggc tgaggcagga961 gagttgcttg agcccgggag gcggaggttg cggtgagccg gggtcgcgtc attgcactcc1021 agcctgagcg acaagagcga aacaaaaaca aacaaacaaa caaaaaaaac ccacccaaat1081 cctttttttt aatgtagtag ggtttatata gatatactaa tataattgca tttggagaat1141 tagagtatgt atggagccca cacatactgt gatataaagt gtatatacag atatttggat1201 attttctagt ttgcatgatg attaagagaa ccagatggga aaatacaatc tccaaagtga1261 tgtttatcct ggaattaccc aatttagatt agagaggttg ttcaaattta actagataac1321 tctagtttgt actgtatagg tgcagttatg acagtaaaaa aatagcctct tggctcatac1381 ctgtaatccc accactttgg gaggccaagg tgggaggatt gcttgagccc aggaattcaa1441 gactagcctg ggcaacataa tacagggaga ccccgtttct attaaaaata caaaaatcag1501 ccagctgtgg tgacacatgc ctatagtccc agctacttag gaagctgaga agggaggatc1561 acttgaggct gtagtgcact ataattatgc ctgtgaatag ccactctact ccagcctggg1621 caacatagca agaccccatc tctaaaaatt aaaaaaaaaa tttaaattag aatatcattt1681 ctagcatctt aggtaggtgc ttatatctgg cttacagaag tctaaggtat tccttatttt1741 tatatctgct gtccacattt atacagctac ataaaaattt tatgacaact tcaacatgaa1801 ccttatattt tcgacaatgc cttgccaagg aatctctgaa gtccatagca ggtcactgtg1861 agacctagtt ccctgttgtc actgacctat gtaatcaaag acagtaatac agcctgggaa1921 acatagcgag accctgtctc tatcaaaaat ttaaaattac ccaggcatgg tgacgcacac1981 ctgtagtcta agtgtccaag ttacttgtga agctgaggtg ggaggatcac ttgagcccaa2041 gagtttgaag ctatggtgag ctatgattgt tccactatac tccagcattg gcaacagagc2101 aagaactcat ctctaaaaag taaaaagcaa ctccccagaa agactgtatt tctacagata2161 aatattgcat tgagatgcca aatagagtgt tgttgtaaag tcatcagact agaaagcaga2221 cctggggaca gtgtttacca cctaagaggc agtcctgttt ttgagaccca catctatata2281 tagagatttt tgtttgtttg tttgtttgtt ttgttttgtt ttgagatgaa gtctcactct
2341 gtcacccagg ctggagtgca gtggcgcggt cttgtgctca ctgcaacctc cgcctcccgg2401 gttcaagcaa ttctcctgcc tcggcctcct gagtagctgg gattacaggt gcgcactgcc2461 acgcctgact aatttttgta tttctagtaa agatggggtt tcactatgtt ggccgggctg2521 gtcttgaact cctcacctca ggtgatccac ccatctcagc ctcccaaagt gctgagatta2581 caggcgtgag ccaccacgcc cggccagaga tccacatcta tatttataac acatttatgg2641 atgaaaatta aacaggtgtc cgggtgcggt tactcatgcc tgtaatccga gcacttgtgg2701 aggccgaggc aggcggatta cttgaggtca ggacttcgaa accagcctgg ccaacatggt2761 gagatcccat ctctactaaa aatacaaaat tagccaggtg tggtgtcacg cacctgtagt2821 cccagttact tgaacctggg aggcagaggt tggagcgagc cgagattgca acactgccac2881 tccagcctgg gtgacagagc aagagaccct gtctcaaaaa aaaaaaaaaa aaaa<210>27<211>2751<212>DNA<213>Homo sapiens<400>271 tgctggccgg gcgagaggct ggcggctggg ctctcgcgcc cctccctgca gggctcaggc61 tctccccctc ctgtcttctc cgcgctgttc ctcgtcatgg cggccctcag caagtccatc121 cctcataact gctatgagat tggccacaga aacactattc agaatgggtg tagcaagagg181 aaccatcaca acattaagaa atggagaagt ccttttgttt tgcatcacag ctgccatgta241 catgttcttt ttcaggtgca aggatggctt gaaaggattt acattttctg cacttaggtt301 cattgtaggg aaggaagaaa ttcccacaca ttctttttca ccagaggcag catatgcaaa361 agtggaacaa aagagagagc aacatgagga aaaacccgga agaatgaata tgattggtct421 agtcaggaaa tttgtggatt caatatgcaa acatggacca aggcatagat gttgcaaaca481 ttatgaagat aattgcatct cttattgcat taaaggtttc atcagaatgt tagcgtgggg541 tacttgatcc agtgctgcct ccgaatccct tctgcattta ggcatctgtt tacacagcca601 tctcggctac tttctctctt ctacaataaa gaaaacttcc agcttggagc ttttcttggc661 tcttttgtta gtatatacaa gggtactagt tgcttcctgc gctggatcag aaacttagat721 gatgaactac atgctattat agctggattt ttggcaggta tatcaatgat gttttataaa781 agcacaacaa tttccatgta tttagcgtcc aaattggtag agacaatgta tttcaaaggc841 attgaagcag ggaaggttcc ctattttcct catgcagata ctatcatcta ttccatctct901 acagcaattt gcttccaggc agctgtcatg gaagttcaga ctttgagacc atcttactgg961 aagttccttt taagactcac caagggcaaa tttgctgtca tgaaccgaaa agtccttgat1021 gtttttggta ctggtgcatc taaacacttt caggatttca tccccaggtt ggatccaaga1081 tacacaactg taacaccaga gttgcccaca gagttttcct gaagatgact gtaacttatt1141 aatgtgacta aatgtttcat cttgaagagt taattatgtt gaacacaaag gagggggccc1201 aagctcgaac ttcagtgtat ttcagttaga gatactcttt tcatttgttt tgtttttctt1261 atgaatcaga aattcagaag ctttttagga agatgttgct taataattaa gcttcctcca1321 tagccagaat aagattctgg atcactgtag tgactgacat tatatattat tgatcaaatt1381 atgtccacaa gcaatattat ataatctacg tagaagtgta ataacaaaca agagtacact1441 taaaattact ttaaaagatg tctttagttc attccaatat aattcttgat taaaattagg1501 attatttcta cattttagga tttacaaagg atcacgggta catggatttg gtctatatat1561 ttttttaaag ttttgaattg gtatctgtag tagtggaatg ttatagattt gaagtaactc
1621 tccacggaca gtgctgcttt cgtgtagagc aatttaattg gagaagtggc cattcttact1681 tcagggatgc aaagatgggt ctcataccat ttggataaat gtcgtggtat ccatgctttt1741 tttcaactaa taacatcatc tctcttcatg accagttaat tgggctattt ggcagcccag1801 tgaacctatg tactaatggc aagttagggg caaatggaaa tggacacatc cgataaagtt1861 gaaatgtatg ttttaatctt tcacagaagt attacacttg aatatttaaa aacaaaactt1921 ttaaacttcc tataggttta tgatgtttgt tttcatttat atggacataa tccttcatag1981 ctcagtttat atgccattgt tgtattagaa gggatcaaaa tcctatggaa caaagtagtc2041 ttggcaagtt ggcagtttgt gtcctctcag ctgtttaact taaggtaatg gatgtttgca2101 cctgaaacca ctataaaaat ccagggttgt ttaaaaagtc catttgtcac taattccatt2161 caggttctcc aaccttcttc ttgaatatca ttgtcaccat ttttactgtt agaataaaga2221 ggtgacacca taaagtcctg ctgataatga gagtagttca ggacagctgt gattgaaata2281 tggtcgctat ttacagtttt tcagggaaaa gttatacttt tctatgttaa taaagagctg2341 aagtggtcta cagttaatgt gacatgtagg gatgatgata tttttaaaat acattttgtt2401 gctaaaaagt ttttaggcca gtgcaaatta tgcagtagaa cttgtgttgc aaaaggaatt2461 ataacccata ctttaaaaat gcttaatccc tcatattcaa tttcatcaag ccttgtatac2521 ttctgcttaa atgtaattca atccttggtt gttatggcaa acagaaaccc aacaaaaaga2581 cagactctgg tactatggta acagagccac tttatcattt ggtcaaaatt tggcattatg2641 attagctttg tagaactgac ctgtttattt ggcaatgctg ttaaaggatc atttcggttt2701 cagacttcaa agttgattaa taaatttaat cttaactttt aaaaaaaaaa a<210>28<211>1242<212>DNA<213>Homo sapiens<400>281 gaagaagaat ttacaggttt taaccaagaa gatctggaag aagaaaaagg tgaaacacag61 gtaaaagaag cagaagattc agattctgat gataacataa agagaggaaa acatatggac121 tttctgtcag attttgagat gatgttgcag cgaaaaaaga gcatgagtgg caagcgcaga181 cggaaccgcg atggtggcac ctttattagt gatgcagacg acgtcgtgag tgccatgatc241 gtcaagatga atgaagctgc tgaggaagac agacagttga acaatcaaaa aaagccggca301 ctgaaaaaat taactttact gcctgctgta gttatgcacc ttaagaagca ggaccttaaa361 gaaacattca ttgacagtgg tgtgatgtct gccatcaaag aatggctctc acctctacca421 gataggagtt tgcctgcact caagatccgg gaggagctgc tgaagatcct gcaagagctg481 cctagtgtga gccaggagac cctgaagcat agtgggattg gacgagcagt gatgtatctc541 tataaacacc ccaaggagtc aaggtctaac aaggacatgg cagggaaatt aatcaatgag601 tggtctaggc ctatatttgg tcttacctca aactacaaag gaatgacaag agaagaaagg661 gagcagagag atctagaaca gatgcctcaa cgacgaagaa tgaacagcac tggtggtcag721 acacccagaa gagacctgga aaaggtgctg acaggagagg agaaggctct tagacctgga781 gatcctggat tctgtgcccg tgcaagggtc ccaatgcctt caaacaagga ctatgttgtc841 aggcccaaat ggaatgtgga aatggagtca tccaggtttc aggcgacctc caagaagggt901 atcagtcgac tggataaaca gatgagaaag ttcacagata taaggaaaaa aagcagatct961 gcacacgcag tgaaaatcag cattgagggc aacaaaatgc cattgtgacc ttgcctggaa1021 tgtgtcccca tctctactct aagaaatgcg caatggactc tttggagaaa gaagatattt
1081 taaaacattt ttagtgtgtc tgtaaatggt tcagcgtgta tcagatgttg tcataggact1141 cacatttctc tcagttatat ttaaaaccgt tgtgtacttt gtacaaagga atactagtca1201 tacttctata aactttacac aataaaattt cattctggtt tt<210>29<211>3323<212>DNA<213>Homo sapiens<400>291 ttggatattg aatctgtaat taccttttat tgtaaatcac gtaacattaa atatagcaca61 tcccttagct ggatacatct actgaaacca ttggtgcatc ttcaactgcc acgcagcgat121 ttatacaact gcttttatgc cataatgaat aagtacattc ccagggattg ttcccagaaa181 gggagaccat ttcatctctt caggttgctc atccaatacc atgagcctga gctttgttct241 tatcttgata caaagaaaat tactccagac tcctatgcac tcaactggct tggaagtctt301 tttgcatgtt actgttccac tgaagtcact caggcaatat gggatggata tctacaacaa361 gcagatccat tttttattta tttcttaatg ttaattatcc ttgttaatgc aaaagaagtt421 attttaacac aagagtcaga cagcaaagaa gaagttatca agttcttgga aaatactcca481 tccagtctga atatagaaga tatagaagac cttttctctc tggctcagta ttattgcagc541 aaaacaccgg cttcttttag gaaggataat caccatctct ttggtagtac tttgttggga601 attaaggatg atgatgcaga tctgagtcag gctctttgtc tggccatctc cgtgtcagag661 atccttcaag cgaatcagct acaaggggaa ggagtccggt tctttgtggt ggattgccgt721 cctgcagaac aatataatgc tgggcattta tcaactgctt tccacttaga ttcagacctg781 atgcttcaga atccatctga gtttgcacag tcagtaaaat ccttgctgga agcacagaag841 cagtccattg agtctggctc catagctggt ggggagcacc tctgttttat gggcagtggc901 agggaggaag aagacatgta tatgaacatg gtcctggcac actttttaca gaaaaacaaa961 gaatatgtga gtattgccag tggaggattt atggcactgc agcagcacct ggcagacatt1021 aatgtggatg gaccagaaaa tggatatggc cattggattg ctagtacctc aggctcaagg1081 agcagtatca attctgttga tggtgaatct cctaatggct caagtgatag aggaatgaaa1141 tcactagtaa ataaaatgac tgtggctttg aagacaaaat ccgttaatgt cagggaaaaa1201 gttatcagtt ttatagagaa tacatcaact cctgtggatc ggcatgtgag cagcagtgac1261 agagtgggca agccttaccg tggcgtaaag cctgttttca gcattgggga tgaagaagaa1321 tacgacacag atgaaattga cagttcttca atgtcagatg atgatagaaa agaggttgta1381 aacattcaga cttggataaa caaaccagat gtcaaacatc attttccttg taaagaagta1441 aaagaaagtg gacacatgtt tcccagtcat ctgttggtta ctgcaacaca tatgtactgt1501 ttaagggaga ttgtttcacg gaaaggattg gcttatatac agtctcgaca agcgctgaat1561 tctgtagtta aaattacatc caaaaaaaaa catcctgaac tcattacctt caagtatgga1621 aatagcagtg cttcaggaat agaaatcttg gcaatcgaaa ggtatttgat tccaaatgcal681 ggggatgcaa ctaaagccat aaaacagcag atcatgaaag ttttggatgc tttggaaagt1741 taatataaaa gaaaattata taaaaagaaa ttaagacaac caagagaaac atggacatat1801 acctcctgac tgaatactaa ctggagacct ttcatttgct catggggctg cttaaatagc1861 aggtctaaga aagtgtaaat tattataatc aatctgtgga cagtaaactt tttaaaaatt1921 tttcttctgc attttggttt tataaaatga tgtattataa aggtcagtta ttaaattact1981 ttgaagtaac tgaccctgtg cccttatgga ctaagtaagg gtacagaatg cagttctgtt
2041 ttgaagagct gttttaaggg aacatgcatc actttcgggt tcaaaaacaa ctgtacacat2101 acatatctgc agtgtcttca ctgaaaatta gagatagaat tagttgaaga gacttcctta2161 attgctacat tgttttactc actgagcaat atcagaaact aaaacataga ttaataattc2221 actcactgtt tctattcttc ttaaaaagag tgaaatcttt ttaatggaat ggttgaagat2281 tcctgccaac tcaaaataca gtacagctag taaaatgttg aaacatcagt ttctaaatca2341 cctttgctcc attagctaat tttttggaca ctaaatgctt caaagataag aaacttcatc2401 ctaatgattt ctaaagagca gttaaaagca ttaaaataag taggtaggct gttaagatta2461 aaattaacat tttggggaca gaattgtgtc tgtaaaaatg tcattggttt tattacaagt2521 ggattatttt attcatattt taaaacaaat gtttaagcca cattgattta tcctcattga2581 ggattatggt ctgtgaccac agtaatatcc acttatataa atgccaaata atcagagagc2641 ttataaagta caattgtttt tgtcatcatt ttctgaattt ctttctcttc ttttggt ttt2701 gtcctctcat tgaagttcca gtggtttgaa attagttttg gacacattgt cttaatgtac2761 tgaaaattgc tctgctgtat tttgcagcgt tcttcattta atttataatg gtccatatta2821 gacacatttg gtaagagaag tccaggaaca catttagggc catggaatag tattttgtaa2881 aatccatttg ggaagttgca atacccacaa tctgacatgt cctaaaatgt aagggattat2941 ctctaagggg ttgatgggag aaatatttag atgtaccctg ttaacagcca gtcattttga3001 tttacttatg gaaatcaagt gaataaaagg caacataatt tgggaaattt tattctaata3061 tctaataaaa acagtttgag tggttgaaga catctgcatg aaattgcacc taggtgcatt3121 acttgatata actctcccct aaaactgttg taattgcatt ttttcccttt tcttggcacc3181 cctttagaaa tgtactgaag tctcattttt cacccatttc agccatggga catttttcat3241 aatattaatg taaagatgtt tgtgttactg tttataaatt acaattgtaa ataaattact3301 agtttgccca aaactttgtt ctt<210>30<211>2672<212>DNA<213>Homo sapiens<400>301 tggcttgatg ccatgttaag aatgatgtga attcttccca gttctgccct ggtgctagac61 attgccccat actttcaatt agacactagc tgtatctaaa tagtcccact cagtaaactt121 acatcttgaa aaacaagacc agtaagaggc cagtgaaagt actaaagaaa gaaaccaatg181 ttgtgtgagt ttcaaagcag ctgcaatgct gtgtaaaagt agagtgttca ttctccattt241 ccaagagtgt ttcagaatag gatgtcttaa gacttcagtc atgtcagaga tttttttttt301 taggtgatta ttgagtttct ccttctcctt taagtcatca ccttcctttt atgaaatgat361 agtaaggaac tcgtctattc tgaaaggcat ttgagaaata gctgaattcc tggctgcttt421 tttgctgggg gtagatggtg gaatacttct ggtctagata taacttacca ctaagaaacc481 cccagtatgt caccactgcc taaatctaac tagaccaggg tccaaatgcc atccaggcca541 ggcaggaaat atacctcatg tgaaagacag taaggagttg tgggcagtgt aacaaacagg601 agagctatgc cccaactaaa aggagcagct gctactgctt agtttcagcc agttgcaaca661 gtatgtggga atgtaggctg catggttgtt aacaagatag atggtaaaaa gatgccagaa721 gatacagaag atagcaaaga atgtggggaa tttggatacc acacatagcg agagacaatg781 aagcatgctt cccagctcgc cagagtgtca cacagctgct cattctgcca cctgccagac841 attaatgtct tcctgcccta cctaaacccc ctctttacct gatattttaa ttcgagactc
901 tagctacatg cccacctact taacaggtac tagtgacagg tacaaaacat tatgggtaac961 aattctgagt gtttaatgca agcccaggtg aagcagggta gcttccatca gcaggtacag1021 acgttacgct gaaaagaggt gcattctgca ttgcactcct ggatctaagt ttctgtattc1081 tcagagcatc aatgcagcaa gcttattgtt cctcaatttt ttacaatatt tatcacaact1141 ctgggagaaa acaaaacaaa tcctatccta tttactattt gtgctaccta gtgaggagat1201 accgctctgt ttagacaaat taaggcactt cacattcttc caccaattga aagttttgta1261 tcttacagtt ctttttttaa ataatatatt tattgagcac tttctatcta ctagtcactg1321 tgatacagta taagtaaagt gggttgtctc atttaatact cagaataacc acatgaagta1381 tgaactgcca ttatctttcc cctttgtaca aatgaggaaa gtgaggctca cagaagttaa1441 ttggcccagg gtcccacaac tagtcagtgc agaggtggga aacataacca gatttgttcg1501 gcatgaactt gtgccaaatt tcctccaaag ttctcaaaag gcaaggcatg ttattttatc1561 ccaatttagc ataccaacaa ctataatact agatatgtag gaaagtgctt aataatcgtt1621 ttttactgat gattcagtgt ctaaattttg aacaaatttg ggtaagatac aagtcacaca1681 taaattgaca gaaaatgtag ttcttcattc aatggttagc agtcattaaa aggtactttc1741 cctttgtttg tggtgataat cagtattagt agttttcata ttatttggct tccatattaa1801 tcatttttat attttcttct ccttcttacc atgtttactt atatcatcca tcttttagaa1861 tcccagggag ctaatttctg gtccctgtgt tgctatcaaa tctgtatctt gcagaaagaa1921 taatttattt caaacaaggg acatacaata gaaagataag acctactgag gtctttttcc1981 catcatttta ttatgaaaaa tgttcaaaca tacagtaaaa ttgaaagaat tttatagtaa2041 atactgacca cggggattct acatcttact ctacttgttt tattattttc ctatccagcg2101 tactttttga tggatttcaa aataaattgc agttgctgat atacttcccc ctagtacttc2161 aactgcagat tattaactag agtttagtat ttatttagtt tttaaatttt tttgatttaa2221 gatttacctg caataaaatg tacaaatctt aagtataaat ttactgagtt cttgcagaca2281 tatacacctg tgtaacccaa accctttcca aaatttagac cattgcaatc atcttcagaa2341 agtttcctaa atccctctcc cagtctatcc cctccccacc cctcaggtat aactactgtt2401 ctcattcttt tatatcaaag gttaaattta cctgttctaa acttcatatg agtgaaatta2461 tacaaaatgt aatgcttctt tcacttagca taatgttttt gagatttatt catgttgttg2521 catgtgtcag tgattcattt ctttttattg ctgagtattc tttcgtatga atatatcaca2581 gtttgttttt ttatctttct gttgatggac actgggctct ttctgcttgt tttttactgt2641 tatgaataaa gctgctatga acattcttag ac<210>31<211>2084<212>DNA<213>Homo sapiens<400>311 ccagctttag ctatgatgca gcaagcacag cagcccctta ccttcattcc ttcttccttc61 ccactttcaa tcaattcatc tattcttttc ctttcttcag actgggcaga gagaaagaaa121 aacagcatca gtatcttctc ctaggcccat cgtgcgtagc ttgatggtct tgagccctga181 ttgcccaggc catgcccacc gggccacaat cggcctcatt tggcatcact ggggatgatg241 ggtccccagt gatggcaaag cccccaagta tccctccttt tctcatcacc catctgttgt301 ggaagatctg tcacctgggg ttcaactgga tcaggaggga aacagtgggg acccaagaac361 agaatggggc tcgtagatat gttctgttgc ccatgcagca cgttaaaaaa tgtccaactt
421 gcccacacct gaaaatcagg cctctgactt cacagaaaat caggtacagt gggccaggcg481 cggtggctca cgcctgtaat cgcaacactt cgggaggccg aggcgggcgg atcataaggt541 cacgagttcg agaccagcct ggcaaatagg taaaaccctg tctctattaa agatacaaaa601 attagccagg tgtggtagga gcctgtagtc ccagctactc gggaggctga ggcaggagaa661 tcgcttgaac ctgggaggtg gaggttgcag tgagccaaga ttgtgctact gcactccagc721 ctgggcgaca cagcaagact ccaccttaaa aagaaaaaag aaaatcgggt acagcagatc781 agaggctgtg ccctttggat gggacacacg cagtccacat ggctctggtc tgatggctca841 tacttctgtt tgggatcgct gagattcacc tgtatggagg ccaccacgat ggatgagaag901 agggcctcca atcccgaggg tcaatacaga cctgaacaga gaactgggag ggggcacccc961 tggatccacc tctcctctaa ggccacccct cctgcaccta cctccatccc taaccctggg1021 ttctactgct ctgccactgc acagatacta cagagcaaaa gggaaccaaa tgaagacaga1081 tcggaagctc caaaccagtg tggctcaccc caaaccagca tgtcttgacg gcatagactt1141 tcaccaaacc agatggcacg tgtcaggagc ctgacaccaa ctgctgagct cagcccattc1201 cccctacaca gaggcccaaa ccagcttgca gcttttccag gcactcaatc cacacctgca1261 atgtgccagg cgctgcagtc tgtgctggga acaatggtga atgagtaacc tcaaggacag1321 tcccaaatcc tgccacctcc tctccatctc cattcccact gcggccctgc agcccagcca1381 cggccccggc cccgctccgg cccccttgct agtcaccagg ctttcactct gaccccaggg1441 aacactcagt tctccacaag gtagccagag gggtctttta aaatgtaaat gaggccaggg1501 gcagtggctc tctcctgtaa tcccaacact tcaggaaagc cgggaggaag gatcgcttaa1561 ggacaggagt tagagaccag cctaagcaac agatccagac cctgtcccta caaaaaataa1621 ataagctagg tgtggtggcg tacacctttg gtcccagcta ctctagaggc tgaggaagga1681 ggaggattgc tggagcccag gaggttgagg ctgcagtgag ccatgactgt gacactgcac1741 tcctgcctgg gcaacagagt gagaccctgt cttaaaaaaa aacagaaaac atgaccaggc1801 atggtggctc acgtctgtca tcccagcact ttgggaagct gaggtgggtg gatcacttga1861 ggttacgagt ttgagaccag cctggccaac atggcgaaac cccgtctcta ctaaaaacac1921 aaaaattagc tgggcgtggt ggcacacacc tgtaatcccg gctactcagg aggctgaggc1981 aggagaatcg cttgaaccca ggaggtggag tttgcagtag gccaagatcg caccactgca2041 ctccagcctg ggagacagag caagactcca tctcaaaaag aaac<210>32<211>1909<212>DNA<213>Homo sapiens<400>321 atatatcgac gaagaatgga aaaagatgtc ccaatgctgc cccaaagcca gagaagaaag61 atggggtgtc cttctgtgct gaacatgtcc gtaggaatgc cctggcactt catgctcaaa121 tgaagaagac caacccaggg cctgtgggtg aaacactcct gtgccagctg agctcatatg181 ctaagacaga gctggggtct cagactccag aaagtagtcg cagtgaagcc agccgaatac241 tagatgaaga cagctggagt gatggggagc aggaacccat tactgtggat cagacatgga301 gaggtgaccc tgacagtgaa gctgatagca tagacagtga tcaagaagat cccctaaaac361 atgctggtgt ctacacggca gaagaagtgg ccctgattat gcgtgaaaag ctaattcgtt421 tgcagtcgtt gtatattgat cagtttaaac gacttcagca tctgctcaag gagaagaagc481 gccgatactt acataatcgc aaagtggaac atgaagctct aggcagtagt ctcctgactg
541 gcccagaggg acttttggcc aaagaacgag agaacttaaa gcgattaaaa tgtctgcgac601 gataccgcca gcgctatgga gtggaagcct tactgcatag gcagttgaag gaacggagaa661 tgctggccac agatggtgct gcccaacagg cccataccac tcgttccagt cagaggtgct721 tggcctttgt ggatgatgtt cgttgttcca atcagtctct tccaatgacc agacactgcc781 ttacccatat ttgtcaggat acgaatcagg ttctcttcaa gtgctgccag ggatctgaag841 aggtaccctg caacaaacct gttcctgtaa gcctctctga ggatccctgc tgcccgctgc901 atttccagtt gcctcctcag atgtataagc ccgagcaggt actgtctgtg ccagacgatc961 tggaagccgg ccccatggat ctgtacttga gtgctgctga gcttcagccc actgagagtt1021 tacctctgga gttcagtgat gacttggatg ttgtgggtga tggtatgcag tgtcctcctt1081 cacctttgct ttttgatcct tcactaaccc ttgaagacca tttagtcaaa gaaattgctg1141 aagacacagt ggatattttg ggccagatgc agatggctgg agatgggtgc agatcccagg1201 gatctcgaaa ttctgagaaa gcctctgcac cattgtctca gagtggattg gctactgcaa1261 atgggaagcc agaacccact tctattagtt gatagtttgg ggaaccattt tactttggtg1321 gatttaaatt tctcgttctt caaagaagta tttctgatca tctctcacaa aacacaggca1381 acccagacta ccttgttttt cttctggatt gagtattata gtcaaacgta aagccaaatt1441 ttgaggacgt ggaccgtggg tttcttccca ctcagggcaa ggaagagaaa gagagataat1501 ccctatcaaa gcttctggga ttatttggga ctatacctaa aaggaaagtg acatctttgt1561 aatgggattt cccaagtcat ggatagaaat ccacttaagt agctaggtgg gagctgtctt1621 tgtgagtggt ctttgagata ctgatggccc taatatcggt tatctctatc tgaagacctg1681 cagtttagct tttcttcacc tctacttaat tgggcagtct ttaacccctt tccctggtga1741 actctggctt tttatcctct gagaaaacag cccacaggac tgggtcctcc ttatccgtct1801 tgcttttaac ctatgtaaat ttctgtggtt gctatcttat gtaaaatata gtaaaaattt1861 attgtatata gtttctatta aatgttaaag aaaatgttat gaaaatatc<210>33<211>2387<212>DNA<213>Homo sapiens<400>331 attcagttct cgagctccag ccctcagcgc atgcgcaaga cgagtcgcct gagggaactg61 atctcagctc gggcccgcgt tacatcctcc tcctcttctt ccttcggccc agctttcctt121 aggggctgca acccggacgc cgaggccggt ttcggagtgg ggagtgccca ttttctctcc181 ttcccacgtt cctggccccc agacgccatt tgcaggcggg tggcttgggt cagcctcccc241 gcccccaccc gactcccgtc acgggagagc gcacaccgcg ccccgagaac caatcagcag301 ccgcgttagg taaccatgtc tgagtctgga cacagtcagc ctggactcta tgggatagag361 cggcggcgac ggtggaagga gcctggctct ggtggccccc agaatctctc tgggcctggt421 ggtcgggaga gggactacat tgcaccatgg gaaagagaga gaagggatgc cagcgaagag481 acaagcactt ccgtcatgca gaaaaccccc atcatcctct caaaacctcc agcagagcgg541 tcaaaacagc caccacctcc aacagcccct gctgccccgc ctgctccagc ccctctggag601 aagcccatcg ttctcatgaa gccacgggag gaggggaagg ggcctgtggc cgtgacaggt661 gcctctaccc ctgagggcac cgccccacca ccccctgcag cccctgcgcc acccaagggg721 gagaaggagg ggcagagacc cacacagcct gtgtaccaga tccagaaccg gggcatgggc781 actgccgcac cagcagccat ggaccctgtc gtgggtcagg ccaaactact gcccccagag
841 cgcatgaagc acagcatcaa gttggtggat gaccagatga attggtgtga cagtgccatc901 gagtacctgt tggatcagac tgatgtgttg gtggttggtg tcctgggcct ccaggggaca961 ggcaagtcca tggtcatgtc attgttgtca gccaacactc cagaggagga ccagaggact1021 tatgttttcc gggcccagag cgctgaaatg aaggaacgag ggggcaacca gaccagtggc1081 atcgacttct ttattaccca agaacggatt gttttcctgg acacacagcc catcctgagc1141 ccttctatcc tagaccatct catcaataat gaccgcaaac tgcctccaga gtacaacctt1201 ccccacactt acgttgaaat gcagtcactc cagattgctg ccttcctttt cacggtctgc1261 catgtggtga ttgttgtcca ggactggttc acagacctca gtctctacag gttcctgcag1321 acagcagaga tggtgaagcc ctccacccca tcccccagcc acgagtccag cagctcatcg1381 ggctccgatg aaggcaccga gtactacccc cacctagtct tcttgcagaa caaagctcgc1441 cgagaggact tctgtcctcg gaagctgcgg cagatgcacc tgatgattga ccagctcatg1501 gcccactccc acctgcgtta caagggaact ctgtccatgt tacaatgcaa tgtcttcccg1561 gggcttccac ctgacttcct ggactctgag gtcaacttat tcctggtacc cttcatggac1621 agtgaagcag agagtgaaaa cccaccaaga gcaggacctg gttccagccc actcttctcc1681 ctgctgcctg ggtatcgtgg ccaccccagt ttccagtcct tggtgagcaa gctccggagc1741 caagtgatgt ccatggcccg gccacagctg tcacacacga tcctcaccga gaagaactgg1801 taagagctct ttgcagaggg ggtcaatttg aggcacccca ccctggtgta gagagaaggg1861 ggcgtccctt agatcggcat gaccttgtga agcccctcct ctccagatcc caggttcctt1921 ttctggaaca gtgatgattg ctactgtctt gtaagcatct cacccatgtg gcaacatgct1981 gtttcctgtg actttgaatc ctcctaatca gccagcatca tgtaggtcct tgtcaccagt2041 tttcctggtg gggaaattta ggctcgggga aaagtgttac atagcaaggc cacacatctg2101 tggctaggag tcatttgatt gctaggagta ggaccagtga ccagatttag cttaaggaag2161 atttattcca gggacctgat cacagaaagc agggccctgc agcagatggg aaccaggaag2221 ccatcagggt ctcattcctc acacttctgc atttgtcttg tttcctgcct ctggctttct2281 ctccctgctt tcacttggcc cggcactagc tctgtggccc ctactttaca ttacttcttt2341 gtccagagct ctcagcatat gagtccaggt cttcctcatc ttacttc<210>34<211>3459<212>DNA<213>Homo sapiens<400>341 atgtgaccac actgaattta atgcatttct tgatttgaag aactccctaa atgaagtaaa61 aaacctactg agtgataaga aactggatga gtggcatgag cacactgctt tcactaataa121 agcagggaaa atcatttctc atgttagaaa atctgtgaat gctgaacttt gtactcaagc181 atggtgtaag ttccatgaga ttttgtgcag ctttccactt attccacagg aagcttttca241 gaatggaaaa ctgaattctc tacacctttg tgaagctcca ggagctttta tagctagtct301 caaccactac ttaaaatccc atcggtttcc ttgtcattgg agttgggtag cgaatactct361 gaatccatac catgaagcaa atgacgacct catgatgatt atggatgacc ggcttattgc421 aaataccttg cactggtggt actttggtcc agataacact ggtgatatca tgaccctgaa481 attcttgact ggacttcaga atttcataag cagcatggct actgttcact tggtcactgc541 agatgggagt tttgattgcc aaggaaaccc aggtgaacaa gaagctttag tttcttcttt
601 gcattactgt gaagttgtca ctgctctgac cactcttgga aacggtggct cttttgttct661 aaagatgttt actatgtttg aacattgttc cataaacttg atgtacctgc taaactgttg721 ttttgaccaa gtccatgttt tcaaacctgc tactagcaag gcaggaaact ccgaagtcta781 tgtggtttgc ctccactata aggggagaga ggccatccat cctctgttat ctaagatgac841 cttgaatttt gggactgaaa tgaaaaggaa agcccttttt ccccatcatg tgattcctga901 ttcttttctt aagagacatg aagaatgttg tgtgttcttt cataaatatc agctagagac961 tatttctgaa aacattcgtc tatttgagtg catgggaaag gcggaacaag aaaagctgaa1021 taatttaagg gattgtgcta tacaatattt tatgcaaaaa tttcaactga aacatctttc1081 cagaagtaat tggctagtaa aaaaatctag tattggttgt agtacaaata caaaatggtt1141 tgggcagagg aacaaatatt ttaaaactta taatgaaagg aagatgctag aagccctttc1201 atggaaagat aaagtagcca aaggatactt taatagttgg gctgaagaac atggtgtata1261 tcatcctggg cagagttcta ttttagaagg aacagcttcc aatcttgagt gtcacttatg1321 gcatattttg gagggaaaga aactgccaaa ggtaaaatgt tctccttttt gcaatggtga1381 aattttaaaa actcttaatg aagcaattga aaagtcatta ggaggagctt ttaatttgga1441 ttccaagttt aggccaaaac agcagtattc ttgttcttgt catgtttttt ctgaagaact1501 gatattttcc gagttgtgta gccttactga gtgccttcag gatgagcagg ttgtagtacc1561 cagcaatcaa ataaagtgcc tgctggtggg cttttcgact ctccgtaata tcaaaatgca1621 tataccgttg gaagttcgac tcctagaatc agctgaactc acaactttta gctgttcatt1681 gcttcatgat ggagatccaa cttaccagcg tttatttttg gactgccttc tacattcatt1741 gcgggagctt catacaggag atgttatgat tttgcctgta ctttcttgct tcacaagatt1801 tatggctggt ttgatctttg tactccacag ttgttttaga ttcatcactt ttgtttgtcc1861 cacatcctct gatcccctga ggacctgcgc agtcctgcta tgtgttggtt atcaggacct1921 tccaaatcca gttttccgat atttgcagag tgtgaatgaa ttgttgagca ctttgctcaa1981 ctctgactca ccccagcagg ttttacagtt tgtgccaatg gaggtactcc ttaagggggc2041 cctgcttgat tttttgtggg atttgaatgc tgccattgct aaaaggcatt tgcatttcat2101 tattcaaaga gagagagaag aaattatcaa cagccttcag ttacaaaact gaacatatgc2161 tttctgagat tcaactttat gatttcttat aatttgccca gtatttgcat cctgttgctc2221 tattaattta aaaacctttt attttgggga aaggccaaca tttgcatcat tcaaagtctc2281 attaattctg gaaaaccatc cattctgatc tctagggtat atacacccac aggcatagag2341 ctcttccacg tggtggaatc tatgcaatga tagatattca cactctaaat atgaggtgtg2401 tgtatgtgta tgggtggcca cagccatgct tacctatgcc atttagttgg tcttacttaa2461 tctgcttaag atttgcatct gtgtaccttt gttcagatta gttttttttt tccagccgat2521 ttcctcttag tggctaatgc tgttagtgaa ttttccaact aatttcctct cattggttaa2581 tgttgttaat gaattgagag aggtaattga ggaaaggaaa tgagtaaatc actgttcagc2641 aacactgatt tccgttaaca catcagttat gaatttcagg gaattcatct cgccagattc2701 ttgataacat gccattcatt gcccttaggt gattgaccct attttcttac atggctcaaa2761 taaaactagt atgctgttgt atgaatcttt tactgaccac accatccaac tataaaaata2821 taacgggaca gctttaaacc aaagatcatg tttagaacaa tgaaaaatta tttgttgtat2881 ctaatacacg cctgtattgt gaaaagcttc atttagcaat gatgtaataa tttttaactt2941 ccaggaaata atctgtgaat ggaaagattt tttaagattt tgagatagtg tttagtctca3001 tgttgggaac acatgaatgt gatgaacata gtgaatacta aagaaaacgc ttcagacttt3061 cagaatgatg gttcagaatt taaaattttt aatcttttct aatttctttt tttcagtgtg3121 aaaatagcac tttaccaaaa gattagccat gaaatggtta ttttgccagt tacatttgat3181 ttcttttgta tctgcaatgt aatgagttat tttatttctt ctgtatttgc agtgtaatga
3241 gtttttgtgg caaagtgtat taagcaattt ttcattatct tgaagttcca caaagtggag3301 aatatttata ttctcacatg cattttaggc acttttgata tgtgaaaata gatgtatttt3361 ctgatgcatt tggttaataa atattaatct gaacattttc atgttctttg ctattttgaa3421 ttccattata gattcatgaa taaagtcatt actagagag<210>35<211>1620<212>DNA<213>Homo sapiens<400>351 ctggacaacc aaaacttaca taggcttttt atcattttac tgggcctatg taactgagtt61 ttagccagtg gaatgtccaa gtgacatttg ccacttctag gtctggcaca ttaacacttg121 atgtattaca tttcagaccc tcttatcatt ctgcagtgtg tttggcgatc tgttgtttaa181 gatctatatg cactggttac tgttgcttgc cttccattcc tacccttact aatcagattc241 ctattatagc tacttctgtc cttgattcat tttgtatatt agatgtgtgt gtgttcatgt301 gcatgcatgc accctcacat gtaggtgtat atgggagtgg tggtaaagac aggatatttc361 ttcagttcat gggattttat ttcaggagaa ttcaaacatt cacgtattgg agaagatgac421 acatttcttg aatgtctttg gctttgagct gaatgcacta actagaaggg actccagatt481 gtcctccttc gggggaggta agtatattct tcatatacag gtaagagtga aatgatagaa541 gaatgttcct cctctccttt tcttcctgga caacagatca aatatttctc agatctttta601 cagctgaatt ggagtcacat ggacaggtat gatcttaaaa tataaaaaat tattttaaaa661 ttacatgcta aaattttata aaaataaatg ctaaaactaa cttaaacgga agcatttgat721 taaaaaattt atccaaaaaa gtttgaattt tatatgaaac aggatagaag atgaaaaagc781 taaagaaata aaatataaat aaattatggc tggggacagt ggctcacacc tgtaatccca841 gcactttggg aggctgaggt gggaggactg attgaggcta ggagtttgag acctgttatt901 tgaggcacat gggaaggaaa gttttctttg agatattcca cattttataa tattcttcaa961 gagatttaca gagttccttg agtgaattgt tggtttgttc ttaatactga atttcctatt1021 caaatttatt tagaagttca tttcttacag tgatctcatc tcctattaag atattcttca1081 aggtaggttc ttggccagtt tctcaatgac agatttttaa aaatattgtt aatcttttta1141 ttaatatgag aacataattg ttccagatct ggaggccatc ctttaagctt cttgcaacag1201 tctgggagca aagagtttac tcgccactgg gtagtgggcc atggacaccc cagtctccac1261 cagaagttcg ggattgcaaa atgggactct ggcagcaaat ttcaaactgt atgccagacc1321 tggccctttg cagagttaaa agaagccagg tgtggtggct cacacctgta atccctggac1381 tttgggaggc tgaggccaga gaatcacctg aggtcaaggg tttgaaacca gcgtggccaa1441 catgacaaaa acccatctct actaaaatag aaaaattagc caggagtgat ggcgggcacc1501 tgtaatccca gctacttggg aggctgatgg aggagaatca cctgaaccca ggaggtggag1561 gttgcagtgg gccaagattg caccactgca ctcctgtctg ggcaacagag tgagactgtc
權(quán)利要求
1.一類在人類肝臟中表達(dá)的相關(guān)基因，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.35所示的序列；(b)SEQ ID No.1～SEQ ID No.35所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.35所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
2.如權(quán)利要求1所述的相關(guān)基因，其特征在于，所述序列包括具有SEQ ID No.1～SEQ ID No.35所示的序列。
3.一種探針?lè)肿樱涮卣髟谟?，所述的探針?lè)肿雍袡?quán)利要求1中所述的序列中約8-100個(gè)連續(xù)的核苷酸。
全文摘要
本發(fā)明公開(kāi)了一類在人類肝臟中表達(dá)的相關(guān)基因序列，它包括SEQ ID No.1～SEQ ID No.35所示的序列；SEQ ID No.1～SEQ ID No.35所示的序列中每條序列的互補(bǔ)序列；與SEQ ID No.1～SEQ ID No.35所示的序列中每條序列有至少70％同源性的序列；上述三種序列中一條或數(shù)條的組合。利用本發(fā)明的人類肝臟相關(guān)基因，可為研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物發(fā)揮重要作用。
文檔編號(hào)C12Q1/68GK1970761SQ20051011059
公開(kāi)日2007年5月30日 申請(qǐng)日期2005年11月22日 優(yōu)先權(quán)日2005年11月22日
發(fā)明者黃健, 韓澤廣 申請(qǐng)人:上海人類基因組研究中心