本發(fā)明涉及分子標(biāo)記，具體地說，涉及與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記。
背景技術(shù)：
棉花是我國重要的紡織原料，是關(guān)系國計民生的重要物資，在國民經(jīng)濟發(fā)展中占有十分重要的地位。隨著人民生活水平的提高，高檔紡織品的需求也日益增長，優(yōu)質(zhì)棉對保障我國棉產(chǎn)業(yè)發(fā)展具有十分重要的意義。因此，改良棉纖維品質(zhì)也是當(dāng)前棉花育種工作中的一個熱點。棉纖維的斷裂伸長率是指纖維拉伸至斷裂時的伸長與原長的比值的百分率。棉纖維的伸長率越大，表示棉纖維的變形能力越好、剛性越差。
全基因組相關(guān)分析(genome-wideassociationstudy，gwas)是應(yīng)用基因組中數(shù)以百萬計的單核苷酸多態(tài)性(singlenucleotideploymorphism，snp)為分子遺傳標(biāo)記，進行全基因組水平上的對照分析或相關(guān)性分析，通過比較發(fā)現(xiàn)影響復(fù)雜性狀的基因變異的一種新策略。
snp(單核苷酸多態(tài)性)主要是指在基因組水平上由單個核苷酸的變異所引起的dna序列多態(tài)性，包括單個堿基的轉(zhuǎn)換、顛換等。snp作為一種重要的遺傳學(xué)工具，具有分布廣泛、數(shù)量眾多、易于批量檢測等優(yōu)點，同時也是功能基因組學(xué)研究的重點對象。snp適于復(fù)雜性狀的遺傳分析，引起群體差異的基因識別等方面的研究，對于植物高密度遺傳圖譜的構(gòu)建、新基因的克隆等具有十分重要的作用。棉花纖維伸長率相關(guān)的snp分子標(biāo)記，在棉花的各個組織和發(fā)育階段均可檢測，不受環(huán)境和季節(jié)限制，不存在表達與否影響，不用分析片段的長度，只需+/-的分析，適于快速、規(guī)?；?、自動化篩查，可以用于棉花纖維伸長率的早期預(yù)測和篩選。
技術(shù)實現(xiàn)要素：
為了解決現(xiàn)有技術(shù)中存在的問題，本發(fā)明的目的是提供與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記及其應(yīng)用。
為了實現(xiàn)本發(fā)明目的，本發(fā)明的技術(shù)方案如下：
本發(fā)明首先提供與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記，所述分子標(biāo)記如seqidno.1～seqidno.297之一所示；其中，在第50bp處發(fā)生突變，導(dǎo)致纖維伸長率出現(xiàn)多態(tài)性。
所述snp標(biāo)記及其發(fā)生突變的等位基因位點如表1所示：
表1與陸地棉纖維伸長率相關(guān)的snp標(biāo)記
進一步，本發(fā)明提供與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記組合，包括seqidno.1～seqidno.297所示的分子標(biāo)記中的任意兩個或多個。
基于上述基礎(chǔ)，本發(fā)明還提供了一種snp芯片，所述snp芯片包含seqidno.1～seqidno.297所示的分子標(biāo)記中的任意一個、兩個或多個。
檢測所述snp分子標(biāo)記或所述snp分子標(biāo)記組合的引物(組)或試劑盒也屬于本發(fā)明的保護范圍。
進一步，本發(fā)明提供了所述snp分子標(biāo)記或snp分子標(biāo)記組合在棉花纖維伸長率標(biāo)記輔助選擇中的應(yīng)用，以及在棉花種質(zhì)資源纖維伸長率的早期預(yù)測或篩選中的應(yīng)用。
本發(fā)明的有益效果在于：
本發(fā)明提供的與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記可以用于棉花纖維伸長率性狀的早期預(yù)測和篩選，還可以用于棉纖維伸長率分子標(biāo)記輔助選擇育種。其直接以dna的形式表現(xiàn)，在棉花的各個組織、各個發(fā)育階段均可檢測到，不受季節(jié)、環(huán)境限制、不存在表達與否等問題，表現(xiàn)為中性(標(biāo)記表現(xiàn)為不受環(huán)境影響，不受發(fā)育時期以及不受表達等的影響就成為中性標(biāo)記)。
snp分子標(biāo)記適于快速、規(guī)?；Y查?；蚪M篩選中snps往往只需+/-的分析，而不用分析片段的長度，利于發(fā)展自動化技術(shù)篩選或檢測snps。
具體實施方式
下面將結(jié)合實施例對本發(fā)明的優(yōu)選實施方式進行詳細說明。需要理解的是以下實施例的給出僅是為了起到說明的目的，并不是用于對本發(fā)明的范圍進行限制。本領(lǐng)域的技術(shù)人員在不背離本發(fā)明的宗旨和精神的情況下，可以對本發(fā)明進行各種修改和替換。
下述實施例中所使用的實驗方法如無特殊說明，均為常規(guī)方法。
下述實施例中所用的材料、試劑等，如無特殊說明，均可從商業(yè)途徑得到。
實施例1
1、419份棉花核心種質(zhì)資源田間試驗于2014年和2015年連續(xù)兩年分別在河北滄州、河南安陽、湖北荊州、江蘇鹽城、甘肅敦煌和新疆阿拉爾共6個試驗點進行。小區(qū)面積6m2，每小區(qū)1行，行距0.8m、株距0.3m，觀察道0.8m，隔離道1.2m，每重復(fù)20株。試驗設(shè)置3次重復(fù)，正常的大田栽培管理。在棉花吐絮盛期，收摘每個正常棉株中部內(nèi)圍第一、二果節(jié)上正常吐絮棉鈴1～2個，共20個，軋花后，皮棉樣品利用hvi1000進行纖維品質(zhì)檢測，數(shù)據(jù)采集參照棉花種質(zhì)資源描述規(guī)范。
2、snp的檢測：取棉株嫩葉，利用植物基因組提取試劑盒提取5ug高質(zhì)量的棉花基因組dna。上述提取的基因組dna送到北京諾禾致源生物技術(shù)有限公司，用于基因組重測序。測序獲得高質(zhì)量的cleandata數(shù)據(jù)量為6454gb，平均每個樣品15.4g，測序深度6.55倍以上。利用basecalling分析及低質(zhì)量堿基過濾，獲得有效原始dna序列數(shù)據(jù)。有效的高質(zhì)量測序數(shù)據(jù)通過bwa軟件比對到棉花參考基因組，比對結(jié)果經(jīng)samtools去除重復(fù)，獲得樣品有效高質(zhì)量序列。采用gatk軟件進行群體snp的檢測。利用貝葉斯模型檢測群體中的多態(tài)性位點，對gatk(26,740,963個snp位點)結(jié)果snps進行過濾，以獲得高質(zhì)量的snps。利用annovar軟件對snp檢測結(jié)果進行注釋。
3、陸地棉纖維伸長率全基因組相關(guān)分析：陸地棉纖維伸長率全基因組掃描(gwas)定位，對步驟(1)所得的陸地棉纖維伸長率性狀結(jié)果和步驟(2)所得的基因型數(shù)據(jù)，采用genome-wideefficientmixed-modelassociation(gemma)統(tǒng)計分析軟件的混合線性模型進行統(tǒng)計分析，具體可參考(http://www.xzlab.org/software.html)。統(tǒng)計模型為：
y＝xα+zβ+wμ+e
y為表型性狀，x為固定效應(yīng)的指示矩陣，α為固定效應(yīng)的估計參數(shù)；z為snp的指示矩陣，β為snp的效應(yīng)；w為隨機效應(yīng)的只是矩陣，μ為預(yù)測的隨機個體，e是隨機殘差，服從e～(0，δe2)。該模型中，通過在μ中加入親緣關(guān)系矩陣來校正群體分析。分析發(fā)現(xiàn)有共計297個snp與陸地棉纖維伸長率性狀顯著相關(guān)，見表1。
其中，參照系的“效應(yīng)值”值為“0”，定義與參照系相比，snp位點突變后棉花纖維伸長率數(shù)值較參照系變大為負效應(yīng)，反之為正效應(yīng)?！坝^測數(shù)值”是指在419份核心種質(zhì)資源中具有該snp位點的資源數(shù)目。
4、結(jié)果
對419份棉花材料進行了2年、6個地點、12個自然環(huán)境的種植，并檢測分析了這些品種的纖維伸長率。通過illuminahiseq測序平臺對這419份棉花品種進行基因組重測序，獲得高質(zhì)量的cleandata數(shù)據(jù)量為6454gb，平均每個樣品為15.4g，測序深度達6.55倍以上。通過gwas分析累計21個計算值(2年6個試驗點共計12個環(huán)境；每個試驗點的年間均值共計6個；6個試驗點每年的育種值共計2個；所有12個環(huán)境的育種值記為1個，上述總計為21個計算值)獲得至少在一個及以上環(huán)境中穩(wěn)定出現(xiàn)的與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記297個(表1)。利用419份棉花核心種質(zhì)資源2年6點共計12個環(huán)境下的纖維伸長率總平均值作為表型值對上述snp的效應(yīng)進行了驗證，結(jié)果顯示67.34％的snp表現(xiàn)出對纖維伸長率變異具有顯著的影響。
雖然，上文中已經(jīng)用一般性說明及具體實施方案對本發(fā)明作了詳盡的描述，但在本發(fā)明基礎(chǔ)上，可以對之作一些修改或改進，這對本領(lǐng)域技術(shù)人員而言是顯而易見的。因此，在不偏離本發(fā)明精神的基礎(chǔ)上所做的這些修改或改進，均屬于本發(fā)明要求保護的范圍。
序列表
<110>河北農(nóng)業(yè)大學(xué)中國農(nóng)業(yè)科學(xué)院棉花研究所
<120>與陸地棉纖維伸長率相關(guān)的snp分子標(biāo)記及其應(yīng)用
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<213>人工序列
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<211>101
<212>dna
<213>人工序列
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<211>101
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<213>人工序列
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<210>30
<211>101
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<213>人工序列
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gtggcaactccctttttttcataaaaccgaaatttcattttcaaattttcaataaatcgc60
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<210>31
<211>101
<212>dna
<213>人工序列
<400>31
aactgtatatgataaaaatgtccgagtgaatgtattaaagaataggatacgattgacacg60
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<210>32
<211>101
<212>dna
<213>人工序列
<400>32
actgatgtggtggaggaatgtattgatactgattatgtgatgcctacggacttttttcgg60
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<210>33
<211>101
<212>dna
<213>人工序列
<400>33
ttaggaagatgagcaatctaagcttggatggtcacaaatatattttgatggagcataaat60
ttgactaagatttcagtttttcaattatttagagtcttcct101
<210>34
<211>101
<212>dna
<213>人工序列
<400>34
gattgttgaaagacttgggagttgttcaaagtcacattaatctatattgtgacggtcaga60
gcgctattcatttagcgaaaaatcaagtctatcattcaaga101
<210>35
<211>101
<212>dna
<213>人工序列
<400>35
gtcctcgaaaatcttaccagtaaataagtttggatatcgttctttcatggcattctcggt60
ttcccaagtagcttattcgactctgtgtttgagccataaca101
<210>36
<211>101
<212>dna
<213>人工序列
<400>36
aaacaagtggactaagccgaactagtcctcatattttgaatacctaggtgagttgtatgt60
aaataatacaactacattgtcaatatatgtgttcgaattga101
<210>37
<211>101
<212>dna
<213>人工序列
<400>37
ttatggaaaaaccaccactattgtagataaactttcacttaataacattgtggacaagct60
accacttttgtagataaattgtcactttattatattatgga101
<210>38
<211>101
<212>dna
<213>人工序列
<400>38
taggatcacccgtccggactagattctttttaccgtcaattccttttcaaagatccatcg60
aaatttcctttcattcaaccgggatttcttcctcttttatc101
<210>39
<211>101
<212>dna
<213>人工序列
<400>39
agtaaatccggatgatttttttcaaaatccgaacacttggcatgtgaaactcccattggt60
aaactatgctatcgtggagatgcactagtcagatagggtat101
<210>40
<211>101
<212>dna
<213>人工序列
<400>40
accacaaccgaggagatgactgaaaaccaagaaaatccgctacctcttgagattgctgtt60
aatcagaatcctgctctgcatactatgtatgattacgctaa101
<210>41
<211>101
<212>dna
<213>人工序列
<400>41
aaaaggatgttttaatgtaggaatatgtaatattggtgaggcacgagctgagcccttgat60
tgccttagtgtttttggctttgattagcctcataaggaaag101
<210>42
<211>101
<212>dna
<213>人工序列
<400>42
aacttagcaaattttcattcattcatttcattacctcttaaggattatagaccacattta60
cattcaaaatataaaacatttacaagtgacaaacaccagac101
<210>43
<211>101
<212>dna
<213>人工序列
<400>43
attggtgaattcgatgctcctaatgctttaatttcatattttatacttatgagagcatag60
gagagcggaaggaacgagaaacgggccaaaaacggagaaaa101
<210>44
<211>101
<212>dna
<213>人工序列
<400>44
gcataaaaattcgtaatcaagataatacgatatgagtacaagaggccgtagacggagccg60
aggaagtgctcgagcaagatcttcgtcttcgagacatatgc101
<210>45
<211>101
<212>dna
<213>人工序列
<400>45
cacggagaaagagctgctagctatgatttttgcattcgataaatttaggtcatatctaat60
attgtctaaagttgtcgtttatactgaccattctactcttc101
<210>46
<211>101
<212>dna
<213>人工序列
<400>46
accctaatcagtcacttaaatcaacacttaatcgtaacttcaacgagatagacaaacctt60
tatccatcaaaagcccttaccttcgatcgaacgatggcgat101
<210>47
<211>101
<212>dna
<213>人工序列
<400>47
ctaaaaggtgtgttgcaacatacaagcaacgtgttgcaacataaggagtggtccactcac60
tcctcaatttaagttggggtcctacacacacaactagcaca101
<210>48
<211>101
<212>dna
<213>人工序列
<400>48
acattctagtgaagaatcctccttgtattcaaaaactagacctctcactcatggtggttg60
attctacacaggctctaatcgtacatgtcggtgtgcaccgt101
<210>49
<211>101
<212>dna
<213>人工序列
<400>49
tagttttgaaaaagatcattcccatacagaaagattttcgaggaaaatggatgcctaatt60
gggaggggccttacgtggtgaagaaagctttttccggagga101
<210>50
<211>101
<212>dna
<213>人工序列
<400>50
gtcttcttcatggagacaaaacttcaaaaaaaaatagatggaacgagttcagagatgttg60
tggattcatgaacggtattgaggttaattctgaaggttcaa101
<210>51
<211>101
<212>dna
<213>人工序列
<400>51
atactggatgaaatttgagcttctcaggcaactcaagcttgtaggccaccttgcctacct60
ttttcagaattcgaaatggcccctcataccaccgaacgagt101
<210>52
<211>101
<212>dna
<213>人工序列
<400>52
gccgaagatacgtctccagcaatgtattcactcgttcggtttgcccatcagtttggggat60
gcatgctagtggaaaagtttaagtctgaacccattaacttg101
<210>53
<211>101
<212>dna
<213>人工序列
<400>53
ccctccaatcgtatactctgaagcatctgtgcgtacctcatacgcctttgaaaaatttgg60
caatgcgagtacaggttcgcttgtcatcgcttgcttcactt101
<210>54
<211>101
<212>dna
<213>人工序列
<400>54
tcggcaattacccgaactttactttcatccattcgaatcgtgccacctcccacaatatgg60
catagaaatggcatttctcgttaggcaaaggagcatttctc101
<210>55
<211>101
<212>dna
<213>人工序列
<400>55
tgccacctctcacaatatggcatagaaatggcatttctcgttaggcaaaagagcatttct60
cctttttgacatatagcttattttctcgtagagtttggaac101
<210>56
<211>101
<212>dna
<213>人工序列
<400>56
tctcgtagagtttggaacacctcccccaagtgtcccacatgctcttcgagcgtcttacta60
tacaccacaatatcgtcaagataaacaaccacaaaacgatc101
<210>57
<211>101
<212>dna
<213>人工序列
<400>57
ctttgggccttcgtcattcttttccctttgatccttgtttcccctaccattgtctttggg60
tttcgacttgggtttggaagaatcattattatcaaacttgc101
<210>58
<211>101
<212>dna
<213>人工序列
<400>58
ggactcctcgagattaaccactcgaatttccaagctcgacagcatatccctcgatcgact60
agcctttctagccctcgcacgggtctccattggctcattct101
<210>59
<211>101
<212>dna
<213>人工序列
<400>59
aaaaaggtttatggcagttaatctcgatatgagcaaagcatacgacaggatggaaaggga60
tttttttaaggaagtgatgtttagaatgggattcaacaaaa101
<210>60
<211>101
<212>dna
<213>人工序列
<400>60
acaatcttttattctgtggttattaatagccacatcggagagaaatttagacctattaga60
ggacttcggcaaggtgacctgttaagcccatttttgtttct101
<210>61
<211>101
<212>dna
<213>人工序列
<400>61
ttttattctgtggttattaatagccacatcggagagaaatttaaacctactagaggactt60
cggcaaggtgacctgttaagcccatttttgtttcttacttg101
<210>62
<211>101
<212>dna
<213>人工序列
<400>62
attagaggacttcggcaaggtgacctgttaagcccatttttgtttcttatttgtggagag60
ggtttatcaagcctaatgagactagctttgaatgagggttt101
<210>63
<211>101
<212>dna
<213>人工序列
<400>63
tttggggaagcaataagcaggggggctaatttattcaaagaaattttacgcgaatataga60
tcttgttcaggccaaagggtaaattttgaaaaatcaacggt101
<210>64
<211>101
<212>dna
<213>人工序列
<400>64
agacatagccatatgcgcgttcattgcacgcaaagcaaatagtgatttttgacttagggc60
atcagcaacaacattagcctttcccggatgatagtcaatca101
<210>65
<211>101
<212>dna
<213>人工序列
<400>65
catggctttctggtgaggatggtggtttcaaattacaaacgttggacaggcagcaaaacg60
gacatgtgtttaagtcaccaaagatgattcttcttgcgaaa101
<210>66
<211>101
<212>dna
<213>人工序列
<400>66
gctgttttgccaactcttcaataatgtttgcggcgttttcacaaattgcaccgctaatac60
ctcacctattgcggcgtttctgctaagcgccgccactgcca101
<210>67
<211>101
<212>dna
<213>人工序列
<400>67
gacaactgaatttttgttttaaaaaaacggtttcgacagcttggcgacttcgctggggac60
tttagagagtcgagccgtgaattggttatgttttgtctttt101
<210>68
<211>101
<212>dna
<213>人工序列
<400>68
gccggtgcttcaaacttccaggctggatcgggctctaaccccgaaaaactccatgctaat60
ttttccattcctgactttgatgaagtggctgaaaaagaaaa101
<210>69
<211>101
<212>dna
<213>人工序列
<400>69
ctgcgtgctaggtcgtgtaactgcttgacttgcaaccctttgaaacctacaggggacaca60
ttgccatgtcccatgcctatgtggacaagaaataggccatt101
<210>70
<211>101
<212>dna
<213>人工序列
<400>70
taactcgagctgccctactcgttgcatttactctggtcaactatcccacatgcatcgttt60
ccttttcattgaagtccgatgtaccacccacctctcccata101
<210>71
<211>101
<212>dna
<213>人工序列
<400>71
agcactctactggcttcttctcacattcggcctccttggcttcgacacctcttgcgctcg60
aaccaagtgccacggccttacctaccggcttctccttaagc101
<210>72
<211>101
<212>dna
<213>人工序列
<400>72
tcttgggtttcttctttccaactcgtggtttcctattaccaccattgtcaccgttgccat60
tgccatccatactatcttccttgtgatctatttcacatatg101
<210>73
<211>101
<212>dna
<213>人工序列
<400>73
ccgttgccattgccatccatactatcttccttgtgatctatttcacatacgccccttccc60
tcggacttggaagacccaagcttgtctttcccttgaccaat101
<210>74
<211>101
<212>dna
<213>人工序列
<400>74
ccccttccctcggacttggaagacccaagcttgtctttcccttgaccaagcttcaccatg60
gattcaactaccatcatggcttccgacagcttttggacacc101
<210>75
<211>101
<212>dna
<213>人工序列
<400>75
tcggggaagcaggattcgccgtcgtagcttcaatctgatccactgcatcactagggaagt60
aagattcgccattgcggcttcaatctttttaattgcaatgt101
<210>76
<211>101
<212>dna
<213>人工序列
<400>76
gtcatccttcgacagaacatagtgatatgagctttaggacaactagtcccattatacttt60
tcaaacttcagcattttaaacttaggcgggagcactaaatc101
<210>77
<211>101
<212>dna
<213>人工序列
<400>77
gtacggatgtatcccgaaagcgattcgctatcctgcacggaggtgaaaacttcacgaagg60
agtagcttctcactcccacttaaagggtgtaaccgagcggt101
<210>78
<211>101
<212>dna
<213>人工序列
<400>78
tacggatgtatcccgaaagcgattcgctatcctgcacggaggtgaaaatctcacgaagga60
gtagcttctcactcccacttaaagggtgtaaccgagcggtt101
<210>79
<211>101
<212>dna
<213>人工序列
<400>79
taaagtggctgatccttggtgcctttactactgcccagatgcgtccccgataatggtgaa60
gtgatattaaccagtttgaaattttcttggttaaaagaaaa101
<210>80
<211>101
<212>dna
<213>人工序列
<400>80
aatccaagtaactcctctggaaacacattggaatactcacaaaccactgacactgattca60
aacttcgattcagacagtttattatccaatgcataagcaaa101
<210>81
<211>101
<212>dna
<213>人工序列
<400>81
ttagactaagaacatgtttatgttcatctactaatacaagttgtcttttcgtattacgat60
tcgaccacgtaataccgcttaatatgtaacagcccgatttt101
<210>82
<211>101
<212>dna
<213>人工序列
<400>82
aactgtctaagtgatgcatgactgggtaggtaaaaacacgttaggggtcactagagtaag60
gactttgcagattgtaaaagggttgtcacatagaacaacga101
<210>83
<211>101
<212>dna
<213>人工序列
<400>83
tccgaatcttggttgtttttacacaattcacttaacaaaattactgaacagcctttttat60
acttttgacttaagacatttctttaacgtttaccataattc101
<210>84
<211>101
<212>dna
<213>人工序列
<400>84
ccgaatcttggttgtttttacacaattcacttaacaaaattactgaactacctttttata60
cttttgacttaagacatttctttaacgtttaccataattca101
<210>85
<211>101
<212>dna
<213>人工序列
<400>85
tatgcatagttttccatgtttttcgcttctctagcgtcctggcttgtccttctcggtgga60
cacacttaaacaacaacacctaaaatataaacataacattt101
<210>86
<211>101
<212>dna
<213>人工序列
<400>86
tgcatagttttccatgtttttcgcttctctagcgtcctggcttgtccctatcggtggaca60
cacttaaacaacaacacctaaaatataaacataacattttg101
<210>87
<211>101
<212>dna
<213>人工序列
<400>87
ctatctttggtagataccttcacattgtcaatttaatgatcgcatacttgtacaccactt60
acaattcttaacgtatcattcatctaactgcttggctatct101
<210>88
<211>101
<212>dna
<213>人工序列
<400>88
cacgaatcaaatacgatgacaatgtcccaaacatggtcttacacgtaaacacaaattgat60
gccaacatcccagacgtggtcttacacgaaaacacatatta101
<210>89
<211>101
<212>dna
<213>人工序列
<400>89
caaatacgatgacaatgtcccaaacatggtcttacacgtaaaaacaaatcgatgccaaca60
tcccagacgtggtcttacacgaaaacacatattagaaatcc101
<210>90
<211>101
<212>dna
<213>人工序列
<400>90
caaaatcatctgaatcaattctgaattcatcggaacacatatttgttttttgaacctcaa60
acaattattggtatcaactcaaaactctatttcaacactcg101
<210>91
<211>101
<212>dna
<213>人工序列
<400>91
cactctcgggtatatcgactgagtctcacaaattttcactcatactctgccactgtcatc60
cgaccctgtttcaattcaagaaattccttgcgtttctgatc101
<210>92
<211>101
<212>dna
<213>人工序列
<400>92
tgaatggtaagttaatttcccgttatacgaacttactaagcataatatgtttacattgta60
ttatttttcccgttttatagtaattcgaaagctcgttgggt101
<210>93
<211>101
<212>dna
<213>人工序列
<400>93
gtaagttaatttcccgttatacgaacttactaagcataatatgcttacactgtattattt60
ttcccgttttatagtaattcgaaagctcgttgggttggaag101
<210>94
<211>101
<212>dna
<213>人工序列
<400>94
cctccaccctctcctgtctttcaatcgattctcgatatgccacaaggtaaagaacttgtt60
agaactgataagcctcctatagataaaattcataaatatgg101
<210>95
<211>101
<212>dna
<213>人工序列
<400>95
tccaccctctcctgtctttcaatcgattctcgatatgccacaaggtacataacttgttag60
aactgataagcctcctatagataaaattcataaatatgggg101
<210>96
<211>101
<212>dna
<213>人工序列
<400>96
taacctcggtaatgtcagtggtagtcgaggtacgataatagattcaactatcaaatttga60
agcacgagcattggcaaggacatatactattcgtgcacatg101
<210>97
<211>101
<212>dna
<213>人工序列
<400>97
tagtttctcaccttggggtgcaccagttttttttgtcaagaaaaaagatggatccctgag60
actatgtattgattaccgacaactcaataaggttacaatca101
<210>98
<211>101
<212>dna
<213>人工序列
<400>98
caacaagtgatcctaatctatcacacacccatgacaatttcctcacctttgtaccttaaa60
gaatgatattctccctaataaattcacccatttgaattcaa101
<210>99
<211>101
<212>dna
<213>人工序列
<400>99
ataaacattgttgtcacaccccagtttaggcgggtttaaggagaaggcgtatggtagtga60
aagaacctatgtttggcgggttgtaattcacccatttgttt101
<210>100
<211>101
<212>dna
<213>人工序列
<400>100
caaccttagtgaccatactgcatgtggtaaatgactagatagaattaaaggagaaatgta60
gttaatgagaacagatgtcaagcaggtgcactctaagtgca101
<210>101
<211>101
<212>dna
<213>人工序列
<400>101
attaccttggcattcaccattaaaaatgaccatttacatatgaccttatctatacatgcc60
acaatcttgtaatcatttacattcataccctttcacaccct101
<210>102
<211>101
<212>dna
<213>人工序列
<400>102
accatttacatatgaccttatatatacatgccacaatcttgtaatcattcacattcatac60
cctttcacaccctcattcggagagctgatactgttgagaaa101
<210>103
<211>101
<212>dna
<213>人工序列
<400>103
accttatatatacatgccacaatcttgtaatcatttacattcataccctgtcacaccctc60
attcggagagctgatactgttgagaaacttcgctggcatag101
<210>104
<211>101
<212>dna
<213>人工序列
<400>104
tgccacaatcttgtaatcatttacattcataccctttcacaccctcatttggagagctga60
tactgttgagaaacttcgctggcatagaccgcctaagtcgc101
<210>105
<211>101
<212>dna
<213>人工序列
<400>105
ggtgcacaagctccgtatccaggctggtgtaaggaggcaacggagggatattgcatatga60
tgatgaaaagaagacacatgacaatatgttctgcctctggt101
<210>106
<211>101
<212>dna
<213>人工序列
<400>106
taatcaagatagcaaatttggctaagatgagatgagaaaagacaatggttaagatgggat60
tttattaggagtaaagcttgaggtgaatacattttgtcaag101
<210>107
<211>101
<212>dna
<213>人工序列
<400>107
gttcctcagataccttcatgcaatctttccaaaatttcttttgcctcgatggcatccaca60
cacctcaacaatacttgatcttttccctttttatacaaaat101
<210>108
<211>101
<212>dna
<213>人工序列
<400>108
tgaactctcgaagcaattcaatgaggtcttgttttacttcttcagctatgcacgtgccga60
tctttaccacctttccctcttccaggatcactgtctctatc101
<210>109
<211>101
<212>dna
<213>人工序列
<400>109
cacgatttcatgtgggaactatccaccggtaacctatttaaggtagataagggtaaaaca60
ttcaaggctgatccattatcaattagcactcctgctaacgt101
<210>110
<211>101
<212>dna
<213>人工序列
<400>110
tcggtcttctaattgttttggtaggtctactcttgttttctctatttctactgcgtcatt60
gagatccggaaccacgagattaataggattatcccctggat101
<210>111
<211>101
<212>dna
<213>人工序列
<400>111
ctctgtgggtacacccttggttgcgtctggacattagttggggcgaaacctagagggtag60
gcagggtcttcgtgatcatccccagaatttactgcggggtt101
<210>112
<211>101
<212>dna
<213>人工序列
<400>112
ctgtgggtacacccttggttgcgtctggacattagttggggcgaaacttggagggtaggc60
agggtcttcgtgatcatccccagaatttactgcggggttct101
<210>113
<211>101
<212>dna
<213>人工序列
<400>113
ttttaaccttgcttcttcattttggaattctaataaacttttctccaagacactctctca60
agctcaggcatcttgaaatttctcttcccactgatcagctt101
<210>114
<211>101
<212>dna
<213>人工序列
<400>114
ttataagaaaactcacactcagctaacccttgtgttgccggtacgaactgtcttgactta60
tattgtcttaacacgagcagaggggcatatctgacagctcc101
<210>115
<211>101
<212>dna
<213>人工序列
<400>115
caaatctgtaacggcttcatctatatgccccaatgctttaggaaaaataaccagcccgta60
catactcaaagcaaaaacatcgactttcttcttcgtatcag101
<210>116
<211>101
<212>dna
<213>人工序列
<400>116
tcatagtaccgttttatttaattacccctattaacacgactcgaactcggacagatacac60
agatgcaaccaacacaccagtttggcacccagtgccttatc101
<210>117
<211>101
<212>dna
<213>人工序列
<400>117
gcaaccaacacaccagtttggcacccagtgccttatcggataattcgaaatagtaaattg60
acacccaatgtctcatcggttaaaccaaagtaaattggcac101
<210>118
<211>101
<212>dna
<213>人工序列
<400>118
tgctttagataaattcagatcttacctattgggaactaaagtaattatcgtttctgatca60
tgcagctttgaaatatctgatagggaagaaggaggcaaaac101
<210>119
<211>101
<212>dna
<213>人工序列
<400>119
agtatcatccacgtgagcgaggtcaatttcgacaaggaagaggattcatacccccggaaa60
acagatttcacgggaatctaacatttacgaatccggtacat101
<210>120
<211>101
<212>dna
<213>人工序列
<400>120
ctgtaccacttatttcgtttacacttttattatcgtcttgactttgcccattgaaccatt60
cagaatcgctagggatactcaaaaatctcgtataactcgca101
<210>121
<211>101
<212>dna
<213>人工序列
<400>121
tgtgataactcattattcactttttgtgaaaactatagctaaaggtagaggcagatgtag60
taacatcctagccagagacagtggtaggtctagctgatgaa101
<210>122
<211>101
<212>dna
<213>人工序列
<400>122
gtcatcttcaaactaaacaaccaaccaccgctttgtcttcttgccattctgccgattgtt60
gctcctaaaccgcatcttgataatgatatatcaacaataat101
<210>123
<211>101
<212>dna
<213>人工序列
<400>123
ctatgcaagaaatttgtatgagtggcttatgttaaacttgaaaattaaacatggttggtg60
tttgggagatgtagactaacgatgtctttttgggatcacca101
<210>124
<211>101
<212>dna
<213>人工序列
<400>124
tgatattatagggaatatgctccgacatggaaaacttcccgttactcgagttccagccgt60
gtagagttcttggaaaaagtgttgttgtaggatttcataat101
<210>125
<211>101
<212>dna
<213>人工序列
<400>125
ttctaaggcagcataatcccagaaggggttaaacgacatttagatcatccttatcctgaa60
gttaatggaagcaagttccattcagcaatacgaccaattag101
<210>126
<211>101
<212>dna
<213>人工序列
<400>126
catcgactcgtagtcgaagtaaccctgaactcttcctatcctatgacataccaactatat60
ctgactctgcccgaacagttaataggatatttaattcattt101
<210>127
<211>101
<212>dna
<213>人工序列
<400>127
actcttcctatcctatgacatgccaactatatctgactctgcccgaacatttaataggat60
atttaattcatttttcaatatcattcaatcaaacatatgga101
<210>128
<211>101
<212>dna
<213>人工序列
<400>128
ttaaagcttcctggatgaagcgagaagggtatttggtgagcctcttttaacaatacctgc60
ctcaaataatttcctgttggcacatacatcttacccattat101
<210>129
<211>101
<212>dna
<213>人工序列
<400>129
ctacctgccatacaggtctgcacgccaggtagtaacacttctttacctttagcagttaca60
agtcgtacgcctctagtttggtagtctacaatgatgttatg101
<210>130
<211>101
<212>dna
<213>人工序列
<400>130
taagttcaattgaacttagtgagttctaaatagacaaaaaaatgattatcagggtactga60
taatcaacctaggaataaagacattttcgagactcaatctg101
<210>131
<211>101
<212>dna
<213>人工序列
<400>131
caaaggtgaaacatgtcggctataggcactcttttatagcactgtcaacagaaaataagc60
ttagtaaagaggttagaaatcccactatcacccttgaaatc101
<210>132
<211>101
<212>dna
<213>人工序列
<400>132
aacctttttacaagccttccataataccctgcctagccgaggaagctacatacctctgta60
aagctcttctgcagattccatgcaggtatagtctagatctt101
<210>133
<211>101
<212>dna
<213>人工序列
<400>133
ttagggtctatcaatgcatatatgttggtgtaaaataaagaaaatttactcgtaataatg60
ttgagggcttcagtgtccacttttttcttacatgacatata101
<210>134
<211>101
<212>dna
<213>人工序列
<400>134
atactaagcggtattatgtgatcggatcataatacagaaagatagttggtattagtagat60
aaacctaaacatgtccttagcctaataaaaaaatgagtaaa101
<210>135
<211>101
<212>dna
<213>人工序列
<400>135
actaagcggtattatgtgatcggatcataatacagaaagatagttggcagtagtagataa60
acctaaacatgtccttagcctaataaaaaaatgagtaaacc101
<210>136
<211>101
<212>dna
<213>人工序列
<400>136
tgctttgattcaattggttcaagcccacactcgaagtaattcatggtacaagaatagcgg60
agaagatcatttagttgaaatttggaaaacattaaggatcc101
<210>137
<211>101
<212>dna
<213>人工序列
<400>137
tacgatttcgctctaagggtttattgctataaatatcacaaactgagtctattttcaaaa60
ttttaattttccactgtacaagaaaaccattttcaaaccag101
<210>138
<211>101
<212>dna
<213>人工序列
<400>138
gagggggcttgggcttacaaaaggctgaaggttgaaatcaaacgcaaaagatgaaataca60
attagaagctcatccacaaaagagatatgttgggctcagat101
<210>139
<211>101
<212>dna
<213>人工序列
<400>139
tgggcttacaaaaggctgaaggttgaaatcaaacgcaaaacatgaaataaaattagaagc60
tcatccacaaaagagatatgttgggctcagatgctctttac101
<210>140
<211>101
<212>dna
<213>人工序列
<400>140
actgcgttattcttattaggctaaaaataattttgattcatagagttaactttatcttgc60
tttgtatatttggggaaggttgcatagtcggtttgttagct101
<210>141
<211>101
<212>dna
<213>人工序列
<400>141
caactccatcccttaagaaatttttgtcctcgaaaaccttaccagtaaagtggttcagat60
attgcttcctcatcgtttccttaggttcccaggtagtttct101
<210>142
<211>101
<212>dna
<213>人工序列
<400>142
ttaacatctgtcgaagaaataatatgtgaaggatctaaccgatactgtcgcaacatagac60
acatgaaaggcattgtgaatcttttctaattctgatagtaa101
<210>143
<211>101
<212>dna
<213>人工序列
<400>143
acatgaaaggcattgtgaatcttttctaattctgatagtaaagctaatcggtatgcaaca60
ggtctaattctctctgtaacttcatataacccaataaatcg101
<210>144
<211>101
<212>dna
<213>人工序列
<400>144
catattagaaataaattgggactaaactaggtattcaaagaatttttcagaaaacattaa60
aatttttcaaaggtgcaagggacacacgcccatgaggttag101
<210>145
<211>101
<212>dna
<213>人工序列
<400>145
ccatgtaactctctgacttgtaacttaattaagtacaggggtcacacggccaagtcacaa60
gcttgtgtgccaggcagtgtgatcaattccaaatactctat101
<210>146
<211>101
<212>dna
<213>人工序列
<400>146
atgtcgagatcaacattccgctgttggacgccatcaagcaaataccgcggtatgctaaat60
ttcttaaagagctttgcaccaacaagcgaaaattaacaggt101
<210>147
<211>101
<212>dna
<213>人工序列
<400>147
cccaatacgttagggtacaattcctcataatccccgaactttgaatatcgcttttatttt60
atgcgaaaatcttcatttcgagaaagtaacaggccacaccc101
<210>148
<211>101
<212>dna
<213>人工序列
<400>148
tgtaatgaaattttcgttattatcagccttagaagatgatttttttttataaaaaatcga60
cattcattcttgaaatgtcccggcttatcacaataataaca101
<210>149
<211>101
<212>dna
<213>人工序列
<400>149
tattgagaaggaggaaagtcaaccaacagttgaaattcctacaccagacgagccagaacc60
tacaaagtctgatgaggtaaaatctaaactagtaaattcgg101
<210>150
<211>101
<212>dna
<213>人工序列
<400>150
tttgaggctaccaaagaaacaatatggcttcgaaagttccttacaaatcttgaagtcgtt60
catggtatgggaaaagttatcatattgtattgtgacaataa101
<210>151
<211>101
<212>dna
<213>人工序列
<400>151
gccaaaatagccaaaaatttgtccatcttctccatggtgaacgaaatcagaaagggggaa60
gccatatttagggttttcaagcttccaagctctatagtaag101
<210>152
<211>101
<212>dna
<213>人工序列
<400>152
tttagactaattttttgttgagaatttgaacttgtaaggagttgctcttagacagatagt60
gggaacctcctaatggattctgatggcttattttttagagc101
<210>153
<211>101
<212>dna
<213>人工序列
<400>153
gcaaacatttgatattttggaccgaggtaagtttgtatgtaaataatgcgttgtttttac60
ttacgctttcatattttgatatattttgtgaaatgtggctg101
<210>154
<211>101
<212>dna
<213>人工序列
<400>154
tgctagcgatttcctatgttacatgatatattaggattcgtattcagatgaggatatgtt60
aacaaatagaagttgaaaatcaagtgtattcaggactaata101
<210>155
<211>101
<212>dna
<213>人工序列
<400>155
gtgaggtccatcgagttctccagtgaggagattttttattcaaatctctttggaagagtg60
gtaatcaccttctcaaccactctgttgtcactgaaatcctc101
<210>156
<211>101
<212>dna
<213>人工序列
<400>156
accagtctctgacactcatagatttcaagtgtctgcaaggattgaaggatggttggcaac60
cttcccagcaatagaggacattctctgattgaaagaaaacg101
<210>157
<211>101
<212>dna
<213>人工序列
<400>157
gagatcaatagtaaaaggaagaaaattcacataaaatgtttgattcccaatcttcaaatt60
tgtatacaactctattctatgtattttccttttttgggttg101
<210>158
<211>101
<212>dna
<213>人工序列
<400>158
gatatcttctatctttatagtgacgaaattgttttgatatgatttcagtcgtgtaatgtg60
aaaggcgaaatgcattgaggaaagagtaggaagattgatac101
<210>159
<211>101
<212>dna
<213>人工序列
<400>159
tttgtacaagggttttcgatgatagctcctcctaatgccacggttgttatagaaagatgt60
gaagtttgaatggaccgataaatgtcagcagaaatttgaca101
<210>160
<211>101
<212>dna
<213>人工序列
<400>160
atggttttctcctgccacctttccgacagagctgagctgacaaaataataagtcactcaa60
agcttttgatgaaaaccatgcaaatctttatacctcaaatg101
<210>161
<211>101
<212>dna
<213>人工序列
<400>161
tcgtttacataaaatcagatttacaaaatattccttatcaaccaatttagaaaaaatgtg60
acgctgccgagacttccgtataccgatccagcttcagaaca101
<210>162
<211>101
<212>dna
<213>人工序列
<400>162
tcaaatgataatatgcagcagagctgacatatatgtcgtactgtaccttacggtaaatcg60
ctgtacagacatgttatagttgaaactgctaggacagatca101
<210>163
<211>101
<212>dna
<213>人工序列
<400>163
ttatgacagttaattatttttatgactcgatttagtggtcccgaaaccatttcccgacta60
gggtcaaattaggggtgtcacaaatctagtccaagagttta101
<210>164
<211>101
<212>dna
<213>人工序列
<400>164
actcgatttagtggtcccgaaaccacttcccgactagggtcaaattaggagtgtcacaaa60
tctagtccaagagtttattgaaatccacaggcaaaaattct101
<210>165
<211>101
<212>dna
<213>人工序列
<400>165
atatatggtatccgctacataatttagtatgcatgagatagttaaaaatttatgtttaaa60
agaatagcgtactccatgctagtatgtaagtgaaatgcatg101
<210>166
<211>101
<212>dna
<213>人工序列
<400>166
gaaggaatggatggaggaataaggaagatgaaattcctattagatattgtcatacggtgt60
tgttgagttcaaaccataatgtgcgatactccgggctttgt101
<210>167
<211>101
<212>dna
<213>人工序列
<400>167
ccatagctcgactatggaaaccaacaaagtccattgggacactaaacacggggtaattcg60
ccaagacgataatgatggaaaaatcttagatatggaaataa101
<210>168
<211>101
<212>dna
<213>人工序列
<400>168
ctatggaaaccaacaaagtccattgggacactaaacactgggtaattcggcaagacgata60
atgatggaaaaatcttagatatggaaataaggttaaaaata101
<210>169
<211>101
<212>dna
<213>人工序列
<400>169
ttatgctaaaagattaatcgtcggtactccaaatagactaatcggtaaaaaaggggttgg60
atagaactcattgacaagaaaatgtacaagaaagtgtacta101
<210>170
<211>101
<212>dna
<213>人工序列
<400>170
atatcatttacccatgttttgggatatgaactccactgttgtaaatgacgctacatactg60
cagatgtcgtacacccaacgcactagctttcagtttcttat101
<210>171
<211>101
<212>dna
<213>人工序列
<400>171
gcgtttaaaaatgagcattagcggcgttttcagaaaacgccgcaaaaaacctaagcccaa60
cggcgtcgttttcagaactttcgtggtctttagcggggttt101
<210>172
<211>101
<212>dna
<213>人工序列
<400>172
tttcagaaaacgccgcaaaaaatctaagcccaacggcgtcgttttcagacctttcgtggt60
ctttagcggggtttttggattagaaaaacgccactaatgat101
<210>173
<211>101
<212>dna
<213>人工序列
<400>173
caaacgaaaatcctcacaaacaactgaaagcatttcatatggtttgtctaagcatgaaac60
ctcagggcgtaactgaggatcaaatcaaattgcgtgctttc101
<210>174
<211>101
<212>dna
<213>人工序列
<400>174
aatactctatgagtttatattacaggggatttagcctggactggtaatctcactgtaagg60
atgaggttcgcgggagtgtgctctctgaaatgaaatgtgta101
<210>175
<211>101
<212>dna
<213>人工序列
<400>175
cgtcggcaggtggggttctttatcagtagtcggtcacggggctgaaacccgcagcatcag60
tccacgcacttcggtgattacaacgaaagcaagacgatagg101
<210>176
<211>101
<212>dna
<213>人工序列
<400>176
ataaaccacaaattaacatcacttaacatcttcaaattataggttttatgagctttactg60
acaagctcatggagcgtaaagttgtcaaaattttgcatcct101
<210>177
<211>101
<212>dna
<213>人工序列
<400>177
acaacaggatgaaagattccagttggccagcatgaccaataaacaaatataaggaacata60
tcataggagatgagcatgataaaaatgttatttctaatggt101
<210>178
<211>101
<212>dna
<213>人工序列
<400>178
agcttcaaaaatctgttgcaaaaacgtcggtctagcttttcattcaacctaaattaaacc60
gtcactaatcaacgtcaatcatgtgttcaaagctcacaaag101
<210>179
<211>101
<212>dna
<213>人工序列
<400>179
tgaaaatcattgagtatatgtgtcattgattatatttttcccctttttgctattttcggt60
cttcattcactgatggatattgctcttatagttggtggaag101
<210>180
<211>101
<212>dna
<213>人工序列
<400>180
ctttgttgagttattttcttgatctctcgaggagagacttaggataaaatggaaccaata60
acccatttctttaatactaaaaaataaattgaacttccaaa101
<210>181
<211>101
<212>dna
<213>人工序列
<400>181
tggcaaggagtacacttcagaaacctttaataggttttgtgaagaggctggaattgagca60
tcagttgacggcgccatacactccacaacaaaatagagtta101
<210>182
<211>101
<212>dna
<213>人工序列
<400>182
agaaacccagaaaatcaaaatcaagttcaaacccttagtattatttactaaaactataca60
taccctaaaatagtagtacccttcccagcaacacctcgggc101
<210>183
<211>101
<212>dna
<213>人工序列
<400>183
tgcaacattagatgtatttatacggctactattaaaataaaatttagaaggagaacatta60
aaaatatatatttctctgtaccttctgtgtttaactgttca101
<210>184
<211>101
<212>dna
<213>人工序列
<400>184
tttcctagcaaagaaattgatgaaggcaagcttatgccaaccaaatataggactttaaga60
tttttcgtttccttaaaaaaggttgttggtattttcatccg101
<210>185
<211>101
<212>dna
<213>人工序列
<400>185
ttcctcaatatccaatccttgcacttggagttggaacacttcttccagttaagcacaatc60
agtcactttcaatttctctaatctttggaacactcccaaaa101
<210>186
<211>101
<212>dna
<213>人工序列
<400>186
gcacaatcagtcactttcaatttctctaatctttggaacactcccaaaaaaaaaggtgaa60
aagatgttcaacaacacatcacattccttaacagtcaactc101
<210>187
<211>101
<212>dna
<213>人工序列
<400>187
agtcaactcttttagcatagaaaaagaattcgtatgaagttgaccgtcccatatcctctt60
tgcattcctcaaatgggggattgtgattttcttcaagttgg101
<210>188
<211>101
<212>dna
<213>人工序列
<400>188
ctgcaacgagagagatgggacttattcctgagttcgtgtaggcatttggattgatgggtt60
ttcttttatattttaggcaccaccatcttggatgccattag101
<210>189
<211>101
<212>dna
<213>人工序列
<400>189
gagcatagacacctcaagaataataattcactgtctgaaagtccaatcaattattcaaaa60
gatgacacttcaaaaacttagatccagtcaacatatataga101
<210>190
<211>101
<212>dna
<213>人工序列
<400>190
agcttgatagtctgatctttaccgtttgatatgaagtaacgaccatcccaacgactgtcg60
aggaatgtaataccttccaggtgtcccaccaaaactcctgc101
<210>191
<211>101
<212>dna
<213>人工序列
<400>191
ggtccaaccaggactcatgatgtgtcgatacatttgaaaattgttcattataaccactgc60
ctgtaagataatcattggcagaagaagctcagatatattat101
<210>192
<211>101
<212>dna
<213>人工序列
<400>192
agctgtgcaacgtgcagctcgtaagaaacgaggtggacttgatggccgaggtttagctac60
tgttgctgttgtaggatacacaaatgctgtaggtggccaaa101
<210>193
<211>101
<212>dna
<213>人工序列
<400>193
acttgtactcttccttggagatcttaccatctttgttggtgtcgaatttttcgaacaccc60
atttcatttcttccacattgggctgataagttcttgaagac101
<210>194
<211>101
<212>dna
<213>人工序列
<400>194
acaacaatgcttccacaccttgcatataggtctatgagggcattactaattaccacatct60
ttgtcaaagcccttgcatatgatataggccatcacagaatc101
<210>195
<211>101
<212>dna
<213>人工序列
<400>195
gacaacatgctgcacatgggcaataaatttagtatacttacttgatttgatctttggttc60
tccttcaccatttgagcaaacaagactagaacatcatttga101
<210>196
<211>101
<212>dna
<213>人工序列
<400>196
gagcaggcagaaaggatgctaacaattgaaattgcatcaggactgaacccttccaattgc60
attttgcgaaatgcaaccaaactttcatccggtagtccatt101
<210>197
<211>101
<212>dna
<213>人工序列
<400>197
tgtgcatacaccggtcgtcgatacggcgtccgcaaatgagtcaggcacgcggacaagttc60
cacatcaaagctttgataactttcatgcataaatacatatt101
<210>198
<211>101
<212>dna
<213>人工序列
<400>198
acaggtgtttgagatgtctgcaacagctctctcatgaatactccctactgaagaacaagc60
ttcaaaccttattcctggtaagtgttgcttcccgttatttg101
<210>199
<211>101
<212>dna
<213>人工序列
<400>199
gtcatatgctttgctcgtgtcaattttaaggcaaacgatccttttctttctaaatgagac60
cttaaaacgaatgcaaaatctcgtaagccaaaatcacattc101
<210>200
<211>101
<212>dna
<213>人工序列
<400>200
cttcgggcacgagtctatagtgtggccataaagttcacaacgaaaatagacattgggtaa60
agactcatattccactctccgtgtttgtccatcaatcttta101
<210>201
<211>101
<212>dna
<213>人工序列
<400>201
tttgaaaacaccgaggctccctttagttgatcaaacaaatcatcaatacacggtaacgga60
tatttattctttattgtcactttattcagctgacgatagtc101
<210>202
<211>101
<212>dna
<213>人工序列
<400>202
tttcttatgtttatagagttttagagatttgctcgggttagaagtcgcaggagaccttat60
tacactatctggtcattacttcggtaaataaaagctttaag101
<210>203
<211>101
<212>dna
<213>人工序列
<400>203
atctatcgcatctttaagaaaaggcaaaatcacaagttctcgaaataccgaaattttagc60
atttcggcttttcggcgaatattaatctaagctactacaag101
<210>204
<211>101
<212>dna
<213>人工序列
<400>204
gtgcatgtctaggattggatccgaagggagcttggtacttaagcagttcgacagactcac60
ctcctcttcttcctggtttcctacctggtgcacagcttcta101
<210>205
<211>101
<212>dna
<213>人工序列
<400>205
ccatcgtattcgctttgaaaatatggcgacattatttatttggtgagaagtgccatgtat60
attcggatcacaaaagtctcaaatatttgatgactcaaaaa101
<210>206
<211>101
<212>dna
<213>人工序列
<400>206
ttgaaaatctatgcatgtttgagatgattagttcaaactctactgatcccatagattaaa60
cttagaattttgaggtttgcaagaggtaagtaccttgaaat101
<210>207
<211>101
<212>dna
<213>人工序列
<400>207
gttaaacccttttactcgttttcacttaaaaccgagtagtacaagctgtctaacataatt60
taaaacgtcatattctatcataaaacaccaaaatacacaaa101
<210>208
<211>101
<212>dna
<213>人工序列
<400>208
acaaaatcgagatgaaagaagatcagacgttgcactgaatctccgtatcgtctcgtccta60
ctctactttacttttactaggcattgattagtagctactca101
<210>209
<211>101
<212>dna
<213>人工序列
<400>209
aatcgagatgaaagaagatcagacgttgcactgaatctccgtatcttcttgtcctactct60
actttacttttactaggcattgattagtagctactcattac101
<210>210
<211>101
<212>dna
<213>人工序列
<400>210
aacaacaacccaaatcgcatccttcttacttgctgacaatggcagtccgaacacaaagtc60
cattgtgactcgatcccatttccactcgggtatcatgatcg101
<210>211
<211>101
<212>dna
<213>人工序列
<400>211
tttaacctcgtatgataagatgaagcatcttgtggttaaacccgactcttagggagaggg60
tctcattgttcgtgggagacaagatcggaatgctgatgatg101
<210>212
<211>101
<212>dna
<213>人工序列
<400>212
ttattttagatcaataccaataataaccttaagttttcaattattaaattcatatatcac60
ttaccaaccctcgttacaccggagaacgtcttatggatatg101
<210>213
<211>101
<212>dna
<213>人工序列
<400>213
gaatttgatgttattttgggtatggattggttgacattgcatgatgctataataaattgc60
aaagaaaaggttatagaattaaagtgtggaaatggtgaaac101
<210>214
<211>101
<212>dna
<213>人工序列
<400>214
tcatccggaaatactacagcagcttgatctgcttcttcatacttcatccagataacttat60
gttgcatggtctgctccttggttgctttatctgctgcatgc101
<210>215
<211>101
<212>dna
<213>人工序列
<400>215
gcaatagcattaagtcagttagaaaacaataaccctttaccatcagtgggcaatttttac60
aaattttatccactataacatgttggcccaggggatttgaa101
<210>216
<211>101
<212>dna
<213>人工序列
<400>216
aacaaattctcttaactctaggatgcccacaaacactcgaatgttttcaatgaggccatt60
ctcaaacctcttacacatagtagcttcagtggatacatatt101
<210>217
<211>101
<212>dna
<213>人工序列
<400>217
ttctcttaactctaggatgcccacaaacactcgaatgttttcattgagggcattctcaaa60
cctcttacacatagtagcttcagtggatacatattctcgca101
<210>218
<211>101
<212>dna
<213>人工序列
<400>218
cttttagggatttcggcttttcctaaaatatgtaagaagactaagttttagagaagactt60
accaccgattccttagtgaatgattccaatgagtttcccac101
<210>219
<211>101
<212>dna
<213>人工序列
<400>219
taatgtggggttttcggctttttgcaacttgtttttagaaaggtaaaacaagagggtttt60
gctatggtcttgtcgtcaaaacttgaagttttagagggctg101
<210>220
<211>101
<212>dna
<213>人工序列
<400>220
accatttcaacaacaatatttttcatagatatgttcatcataaaaacaatagaaattaaa60
aagaaagggaacagaaagcaaatccagtgtttctctgcggc101
<210>221
<211>101
<212>dna
<213>人工序列
<400>221
cagtcatcgaagaaatttagagatgatttgggccgatctagagacactttgggtttttct60
agacgagatcgcgatcgaccccctgtgagtgcacgagtcac101
<210>222
<211>101
<212>dna
<213>人工序列
<400>222
aatattaaaagaagaccccataaagcctatacccatattcggccatctctctaaattata60
gggtttcggcttttggccaacagttactctaggaatcgttt101
<210>223
<211>101
<212>dna
<213>人工序列
<400>223
ggttaaagttctaagaaggaagtaggttccgttggcaaaagtactatggtgtaatcatgg60
aagggaagaagctacttgggaatcagaagagactatgcgtc101
<210>224
<211>101
<212>dna
<213>人工序列
<400>224
ttgtggatgaaaagcggtgctaaaatgcagcttggtacccaaagcttctggcaatttctt60
ccaaaatcgcgaggtgaatctcggatctctatccgacacaa101
<210>225
<211>101
<212>dna
<213>人工序列
<400>225
agcattgatcagcgatcgtggaacgtatttttgtaataaggtaatcgacacacttttgaa60
aaaaaaggggtagtgcaacgagttgctacgacttatcaccc101
<210>226
<211>101
<212>dna
<213>人工序列
<400>226
tgtacgtgcatgataaacttgattctttgctaaatgaatagcactttgattatcacaata60
cacgttaatatgctcctggaccaatcccaaggttttagtca101
<210>227
<211>101
<212>dna
<213>人工序列
<400>227
aaaacgatgtttactcccttaagtttgtttgatgatgatgggattttggatgagttgcaa60
gttaagccatcttggttggatgagattaagagtaagcagct101
<210>228
<211>101
<212>dna
<213>人工序列
<400>228
tatacattcatggcatacatcatacagatttaattcatcatgcttttcatacaaaattaa60
ccctaggggtataaaggtcattttgcatacataggggtatt101
<210>229
<211>101
<212>dna
<213>人工序列
<400>229
gcagaatggaaggaagaaattagcaatatcaagggaggaatggagttttagaagggaaaa60
gcaaagaaagaggaggaaaaggctgcgcgcgctgtgataga101
<210>230
<211>101
<212>dna
<213>人工序列
<400>230
aaggtccatcgccttcgatctatttccctactgcttagggggttaagatctgtaaattca60
gcctgttaccctactgcttaggggtttaagacttgtcatct101
<210>231
<211>101
<212>dna
<213>人工序列
<400>231
gaaccaatcattcatcacgctatagaaagcttgtctagctccatcattcggattactagc60
attaggttgagagtccgccggcgctgtctcttgtgcgggag101
<210>232
<211>101
<212>dna
<213>人工序列
<400>232
cgaggtagcactcaagctggatctgcgtcatctgaacacataccagctgacgaggtacgt60
ctaccaccggttaatgaaaatgagccgtatgaccgggccgc101
<210>233
<211>101
<212>dna
<213>人工序列
<400>233
attatgtgataaggccgaatggccaatgtgatggatgtgaaagtgtataaatgtgataag60
gccgaatggccaatgtgatgaatgtgaaagtgtataaatgt101
<210>234
<211>101
<212>dna
<213>人工序列
<400>234
ccttcggggttacttcagccaattactataccagaatggaaatgggaaacaatcactatg60
gattttgtatcggggttacctttgtctctgaggaaaaaaga101
<210>235
<211>101
<212>dna
<213>人工序列
<400>235
acagtaggccagaatacttgtcattattctgctataattctccttacattatattttctc60
atgttcatgtttttggttcaacacttttctcatgttaatat101
<210>236
<211>101
<212>dna
<213>人工序列
<400>236
aaatgaatacattcagtatgttaagtgggccaggtacgcattatgtacttatatgtgagt60
ttgatctgcagtgaatcataagtgtgcatagtgatacaccg101
<210>237
<211>101
<212>dna
<213>人工序列
<400>237
tacttgttcttattcttaattgtgagatttaatccttgttttaatattttaggatattaa60
ttcaggttttgatgtgcttattcagttgagcaaaagtccct101
<210>238
<211>101
<212>dna
<213>人工序列
<400>238
catcctgcttaaagcgtttttgacactatttgcttagaaacatctactcgaaattatttt60
ttcaagagcaattgtagcaaaagcgcgtccacgtgggagct101
<210>239
<211>101
<212>dna
<213>人工序列
<400>239
atataaatttcctcttccaaatcaccatgtaaaaatacagtcttcacattgagctgaact60
agttcaagatcatattgcgcaaccaaggctagcaaaatccg101
<210>240
<211>101
<212>dna
<213>人工序列
<400>240
cgtattaaggactttttgagtgtatatgaatgatgttatgattaattgaggattgtatgt60
gaaatgattttctaaattatgagataagtctggtaatgctc101
<210>241
<211>101
<212>dna
<213>人工序列
<400>241
ggtagctacggtggtggtggtaagtcctctggtttattattgttaatctcattaaatagt60
gggctatctcacggtgggaaatttcctacagcaggtggttt101
<210>242
<211>101
<212>dna
<213>人工序列
<400>242
taaatgtgtaacagccttgaattgtaaccactataagctaaaaagtcacatgcaataaac60
acattaaaatgagtttattaaacaaatgaaaacacttatta101
<210>243
<211>101
<212>dna
<213>人工序列
<400>243
aatataaatttgaagctttctcttccatcttttgatgttcttctgacttctgctgaacaa60
ccacctcggaggcaatactcctactgatttgatcgccaacg101
<210>244
<211>101
<212>dna
<213>人工序列
<400>244
tttcttcatctactaaaaagaacaacacaattttagctttagtgacaatgtttcttcatt60
tctaataacctcccttcttatcttgctcttcatgttatcag101
<210>245
<211>101
<212>dna
<213>人工序列
<400>245
tgttcgatcggtaagggttagttattggacgttccataactaatttacataaggaagagt60
tggtgtccgaggcatccttggtagctataactagcttattg101
<210>246
<211>101
<212>dna
<213>人工序列
<400>246
aaagtcacggaatgggtaagtcgccaatccctaaaacggactatgtaatgtactttatcc60
tctgggttacgggggggcattttaccagaggtttatattgt101
<210>247
<211>101
<212>dna
<213>人工序列
<400>247
ttccgacccttcttcactaaaaattaattatctctttgtacaaaatttgtatgatgtttc60
catttgtttatattgaaaatatactaattcaacattttaaa101
<210>248
<211>101
<212>dna
<213>人工序列
<400>248
ccctaaactttaaactccaaatccaacctcgaacctcaaatcctgaactcgaaccttgaa60
tcttgaactcaggtttcgggatttgaggtttggggttcaga101
<210>249
<211>101
<212>dna
<213>人工序列
<400>249
ggtactgaagcttcctctcgagcacgaatcacataagccctagctagtggccatgcctta60
gatctaacagcagaatctttcattccacctcaattgttacc101
<210>250
<211>101
<212>dna
<213>人工序列
<400>250
ctcaacagtttagtataaagcacttgagagaaactaaatttgttctagacaaaaggatcg60
taagaacaaaaggatagcattatcacaagcttttaaaacat101
<210>251
<211>101
<212>dna
<213>人工序列
<400>251
tttcactcttatatcttgtgtttctgtcttccatgttttacttacgtatcgaaattgtaa60
caatcaaatgttagctcattgattgtctaagtttaaactga101
<210>252
<211>101
<212>dna
<213>人工序列
<400>252
tagtctttattgattactactccatatatgggtattttacctgatgcactgcaaaagtga60
aacctttgataaattcaaagagttctgtgcgaaagtggaga101
<210>253
<211>101
<212>dna
<213>人工序列
<400>253
gctttatgtctccatctgtctaaatacaaaaatctctcatcttagttgatctgctttgtt60
gattgggaaaaacttctgcaagaactttccggtgagttcat101
<210>254
<211>101
<212>dna
<213>人工序列
<400>254
gcagtggagctggttaaagatggtagatcttgccttcctgcattaacagcgaagcagatc60
gaaaacaaagtcttgcctccatcggttgcagtggagctggt101
<210>255
<211>101
<212>dna
<213>人工序列
<400>255
ccaagacgacaagacgaaggcagcccctcaacgaggcccagacattcactcggctcatca60
tcagcggccatgcaatcaccaggcctaacgagagcaccgac101
<210>256
<211>101
<212>dna
<213>人工序列
<400>256
tatacacccacttaacacgtatgaccttcttgtgttctggtctgttcaccaattcccatg60
tctgattcttgtcaatcatttccaacttagcctccatagct101
<210>257
<211>101
<212>dna
<213>人工序列
<400>257
cttagctaaccgctgcttaagcattgtagtacgatgagacctacaagctttaatgccctt60
actctagttacccaaatcaacatccgtttgtttcaatttgg101
<210>258
<211>101
<212>dna
<213>人工序列
<400>258
agaacccaaccagttttgaaacggaacctccaagagcgccctatataagccatgtgggtt60
gtatctaacaatatcaatggcagtaatcagaaaaaactgtg101
<210>259
<211>101
<212>dna
<213>人工序列
<400>259
aacccaaccagttttgaaacggaacctccaagagcgccctatataaggcgtgtgggttgt60
atctaacaatatcaatggcagtaatcagaaaaaactgtgcg101
<210>260
<211>101
<212>dna
<213>人工序列
<400>260
ggcatgtgggttgtatctaacaatatcaatggcagtaatcagaaaaaaccgtgcggaagg60
tgttgcaaagaataattaggaaactcatccttctattcagc101
<210>261
<211>101
<212>dna
<213>人工序列
<400>261
tctattcagcattagcaagcccactatcaagcctttctcggatattattgttcgacaatc60
tgcctcattcccatgtgaaccagcttccagagtatgcaata101
<210>262
<211>101
<212>dna
<213>人工序列
<400>262
ctattcagcattagcaagcccactatcaagcctttctcggatattattagtcgacaatct60
gcctcattcccatgtgaaccagcttccagagtatgcaatat101
<210>263
<211>101
<212>dna
<213>人工序列
<400>263
tattcagcattagcaagcccactatcaagcctttctcggatattattatccgacaatctg60
cctcattcccatgtgaaccagcttccagagtatgcaatatc101
<210>264
<211>101
<212>dna
<213>人工序列
<400>264
gaaataagcatgatctggtccctctccaccgcttggcatagtctgtcgagtaaaattaag60
ctttttcggaaaagccaagtcttccttccaaaatcagttcc101
<210>265
<211>101
<212>dna
<213>人工序列
<400>265
ccctaaagttgtctgtgttatacagcttttctgtccaaaccgtacatataaatgtaggtt60
tggaacttggagccaccatcgagcctttgacagataactga101
<210>266
<211>101
<212>dna
<213>人工序列
<400>266
aagaataaattaggttttgttggtggttcctattctaaagaattgttgccagtcgatttg60
tatccacaataggatagatataattctctagtgttgtcatg101
<210>267
<211>101
<212>dna
<213>人工序列
<400>267
tgattaaatatccatagagcacattgggacaaactctatgatagtgcatatgctgacaaa60
aggtttaccacctaaagtcttttatgagtacactgctcata101
<210>268
<211>101
<212>dna
<213>人工序列
<400>268
gcttctactgctccgttcatcttcgggcggtagggtgaagaattatgatactttatctgg60
aattgctcacatacttctttcatcatcttgttgttcaaatt101
<210>269
<211>101
<212>dna
<213>人工序列
<400>269
cttagcattttgtcatatgtgtcgtattagttagccaatgtgatggctcgttttggtata60
ttattccattttgtatatggccatgaaacatggctcatttt101
<210>270
<211>101
<212>dna
<213>人工序列
<400>270
cgtaaccctattccgacattgaatatgggtgaggggtgttacaagtctggccttataagc60
ttttactattttatttgcatttcttttctttttatagatct101
<210>271
<211>101
<212>dna
<213>人工序列
<400>271
tgggtcagataatttttctcatgtggcttcagttgtctcgacgcataagctacaactcga60
ccctcttgcatcaatacgcaacccaacccaagtagggatgc101
<210>272
<211>101
<212>dna
<213>人工序列
<400>272
tcgcgtaggttatcactactcgaagtttatcaaggttttgtgactgatgagctacaatgc60
cttctcgcttgactgatttttctgaagaaacatttagccag101
<210>273
<211>101
<212>dna
<213>人工序列
<400>273
caaagtccgtcccaaaataataaagatggtgcatgtgagagtcaagttttagagggcgtg60
ttagggaaattgcacatgcaatatggtagttgacgccacaa101
<210>274
<211>101
<212>dna
<213>人工序列
<400>274
agtgaaacataattgaaatgatccaccgcctcattaaaaagataaaaagaaaaaaaaaaa60
gcatttacttacgatgagggtattgaaattccatcttgctt101
<210>275
<211>101
<212>dna
<213>人工序列
<400>275
gtccaacggttttaaagtgttaatttgagatctgtaagagggtttctgtagtgctgaatt60
atgtgtggtattttttatattgcggcagggggggcatgtgg101
<210>276
<211>101
<212>dna
<213>人工序列
<400>276
cagtatatcacatcctctatgagatatgaagcaattttacccaaaaatcaatttgaatgt60
aaaatcaataattacaacttcgacttgtacctgagaatata101
<210>277
<211>101
<212>dna
<213>人工序列
<400>277
gaattatccagtatccactcatgaaatttctgatttctctttggatgtaaagggagatgc60
tgacagggcttgagtcagagtgaattgttctccttttcaac101
<210>278
<211>101
<212>dna
<213>人工序列
<400>278
attcatcagctagcaactcagttctttcaaacaaatcccgaaactataaaatcaacccac60
cgaaatcaccaaatgcaaatttcaaatcacacagttcacgc101
<210>279
<211>101
<212>dna
<213>人工序列
<400>279
aatcgggctgctgtttatttggaaatgggcaaggtatggttatttatttttatttgttta60
gaagctactataaaatgtacttctttcgttgtagtttttgc101
<210>280
<211>101
<212>dna
<213>人工序列
<400>280
agctcaaaagctgaacagaagctcgtaagagctaaacaaaaactcataatggttgagcag60
aagctcataagagctgaatggaaactcgtataagttaaaca101
<210>281
<211>101
<212>dna
<213>人工序列
<400>281
tcaatggagaaggattccatatttgggttgtcaaaatgaagacttacctacaggcattcg60
acctatgggaggtagtcaattcagatgcagagccagcacct101
<210>282
<211>101
<212>dna
<213>人工序列
<400>282
tgattcgttatcacttaccatgaagaaattgatgctactatctatgtggtttaataagtc60
atttaaataatatcagcttgcagttgttttcctttgatatg101
<210>283
<211>101
<212>dna
<213>人工序列
<400>283
tgctcgatttgtggaaatgctgcaggttgctgtggcttctggttgagcttctccaaattg60
aaggcaacgtctactcatgctgcgttggagttcactggcct101
<210>284
<211>101
<212>dna
<213>人工序列
<400>284
aataatgagctgtaataaacctgactaacctttttacaaatctgagcaacctctttctga60
accatttggtatatatctgaagacttatatgacttcaccag101
<210>285
<211>101
<212>dna
<213>人工序列
<400>285
agggaagtcatccaatcttggagtaggtaatgtagagtcactagttttctttcatccccc60
tactagcaaggcaacctcaattgctactggcctcaccatgg101
<210>286
<211>101
<212>dna
<213>人工序列
<400>286
ttgttttcaggtttacaattcactgtttcgctctgtgtttgaaatctatggatatgggtc60
tgtctgtttctttttggttgctttttcggagactgatttca101
<210>287
<211>101
<212>dna
<213>人工序列
<400>287
ttgaaaacgccttttcgtaccattcatgaggtaagacctctttgtcggagtcccttgtgc60
agtgatataacattgcattaaaatgcagagcaccatttagg101
<210>288
<211>101
<212>dna
<213>人工序列
<400>288
tggctgcgttcagtgtgcctcttgaccaaagtgatgatgaagcagtaacagcattgtgta60
ttgaaggcttccgctgtgcaattcatatcactgctgtgatg101
<210>289
<211>101
<212>dna
<213>人工序列
<400>289
atgatgaagcagtaactgcattgtgtattgaaggcttccgctgtgcaatacatatcactg60
ctgtgatgtgcatgaagactcatagagatgcatttctgact101
<210>290
<211>101
<212>dna
<213>人工序列
<400>290
aacaggtgatcatgctttcattttcaacgtggcttgcatggtttttagccggaaagctcc60
atggttaccctgaatcatggataccatcttcaatggatagt101
<210>291
<211>101
<212>dna
<213>人工序列
<400>291
ctgttatccgatcaaaattatctaaatcccaaaaatccttttttttttgttgctcaataa60
gcagagttcgggggtgtgaaaatggcttctgatgtgccttg101
<210>292
<211>101
<212>dna
<213>人工序列
<400>292
cacttaggatgcaccctactttgtgaggaacaagcaagataaaatatacgaattggcctt60
tgtttattatgcttatcatcggccctaacaaggagtcccag101
<210>293
<211>101
<212>dna
<213>人工序列
<400>293
atgcaccctactttgtgaggaacaagcaagataaaatatacaaattggcgtttgtttatt60
atgcttatcatcggccctaacaaggagtcccagtctaacta101
<210>294
<211>101
<212>dna
<213>人工序列
<400>294
cagagtatgcaatatccaccaaagtcatcccaatacttgtctgaatgccatcatttcgtt60
ctcgtcccgtaaccgaccaaccagcggttgcagggttgaga101
<210>295
<211>101
<212>dna
<213>人工序列
<400>295
atgcaatatccaccaaagtcatcccaatacttgtctgaatgccgtcattccgttctcgtc60
ccgtaaccgaccaaccagcggttgcagggttgagaaagggt101
<210>296
<211>101
<212>dna
<213>人工序列
<400>296
gtctgaatgccgtcatttcgttctcgtcccgtaaccgaccaaccagcggctgcagggttg60
agaaagggtgcggagaaggttccaagagtcggaacgaacgc101
<210>297
<211>101
<212>dna
<213>人工序列
<400>297
ttgcagggttgagaaagggtgcggagaaggttccaagagtcggaacgaaagcgattatct60
gtctataattttttataactgagagaagtattgaaatggtt101