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一種陽性質(zhì)粒分子pBI121-Screening及其應(yīng)用_5

文檔序號:8334125閱讀:來源:國知局
ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg 12960 gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg 13020 attctgtcgc tactgattac ggtgctgcta tcgattaatt cgggggatct ggattttagt 13080 actggatttt ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa, 13140 iacataclaa gggtllctta tatgctcaac acatgagcga aaccctatag gaaccctaal 13200 tcccttatct gggaactact cacacattat tatggagaaa ctcgagaagc ttgcatgcct 13260 gcaggtcccc agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga 13320 gaggcttacg cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc 13380 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag 13440 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa 13500 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaa.aaa ggtagttccc 13560 actgaatcaa. aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta 13620 aagartggcg aacagttcat acagagtctc ttacgact.ca atgacaagaa gaaaatcttc 13fi80 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca 13740 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga 13800 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc 13860
[0152] tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt 13920 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc 13980 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa 14040 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga 14100 acacggggga ctctagagga tccccgggcg ataagaaaag gcaatttgta gatgttaatt 14160 cccatcttga aagaaatata gtttaaatat ttattgataa aataacaagt caggtattat 14220 agtccaagca aaaacataaa tttattgatg caagtttaaa ttcagaaata tttcaataac 14280 tgattatatc agctggtaca ttgccgtaga tgaaagactg agtgcgatat tatgtgtaat 14340 acataaattg atgatatagc tagcttagct catcggggga tcctagaacg cgtgatctca 14400 gatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca 14460 ga.aacccacg tcatgcca.gt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg 14520 ggca.ta.tccg a.gcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc 14580 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga 14640 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc 14700 gta.ggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac 14760 ctcggcgacg a.gccagggat agcgctcccg ca.gacggacg a.ggtcgtccg tccactcctg 14820 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat 14880 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc 14940 tgggttcatc tcaggattta gcagcattcc agattgggtt caatcaacaa ggtacgagcc 15000 atatcacltt attcaaattg gtatcgccaa aaccaagaag gaactcccat cctcaaaggt 15060 ttgtaaggaa gaattctcag tccaaagcct caacaaggtc agggtacaga gtctccaaac 15120 cattagccaa aagctacagg agatcaatga agaatcttca atcaaagtaa actactgttc 15180 cagcacatgc alcatggtca gtaagtttca gaaaaagaca tccaccgaag acltaaagtt 15240 agtgggcatc tttgaaagta atcttgtcaa catcgagcag ctggcttgtg gggaccagac 15300 aaaaaaggaa tggtgcaga.a ttgttaggcg cacctaccaa aagcatcttt gcctttattg 15360 caaagataaa gcagattcct ctagtacaag tggggaacaa aataacgtgg aaaagagctg 15420 tcctgacagc ccactcacta atgcgtatga cgaacgcagt gacgaccaca aaagaattcc 15480 ctctatataa gaaggcattc attcccattt gaaggatcat cagatactca accaatcctt 15540 ctagagcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg tagga.gccac 15600
[0153] cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga 15660 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg 15720 tatctttgat atttttggag tagacaagcg tgtcgtgctc caccatgttg acgaagattt 15780 tcttcttgtc attgagtcgt aagagactct gtatgaactg ttcgccagtc tttacggcga 15840 gttctgttag gtcctctatt tgaatctttg actccatggg aattcaatga aaaagcctga 15900 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgaca.gcg tctccgacct 15960 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 16020 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt algtttatcg 16080 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga 16140 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 16200 aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc 16260 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac 16320 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt 16380 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc 16440 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct 16500 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc 16560 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca 16620 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta 16680 tatgctccgc attggtcttg accaactcta tcaga.gcttg gttgacggca atttcgatga. 16740 tgcagctlgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg 16800 gcgtacacaa. atcgcccgca. gaagcgcggc cgtctggacc gatggctgtg tagaagtact 16860 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agtgtgtttt 16920 actcctcata ttaacttcgg tcattagagg ccacgatttg acacattttt aclcaaaaca 16980 aaatgtttgc atatctctta taatttcaaa ttcaacacac aacaaataag agaaaaaaca 17040 aataatatta atttgagaat gaacaaaagg accatatcat tcattaactc ttctccatcc 17100 atttccattt cacagttcga tagcgaaaac cgaataaaaa acacagtaaa, ttacaagcac 17160 aacaaatggt acaagaaaaa cagttttccc aatgccataa tactcgaaca tgcaaaaact 17220 cattaactca gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg 17280 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag 17340
[0154] cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag 17400 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct丄7460 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa 17520 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg 17580 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc 17640 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgcaggtgc 17700 acatccggcg attgctca'ct ttttacaaca gcctgatgcc gaacgtttaa gcgaactgtt 17760 cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga tlttaaaatc 17820 ggccctcgat agccagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta 17880 cccggaagac agcggtctgt tctccccgct attgctgaat gtggtgaaat tgaaccctgg 17940 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga 18000 agtgatggca aactccgata acgtgctgcg tgcgggtctg acgcctaaat acattgatat 18060 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca 18120 gccggtgaaa caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc 18180 gctgcatgac cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt 18240 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg 18300 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt 18360 agcgcgtgtt tacaacaagc tgtaa; 18385 <210>2 <211〉1176 <212> Sequence of PMI gene <400〉 algcaaaaac tcattaactc aglgcaaaac tatgcclggg gcagcaaaac ggcgttgact 60 gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca 120 catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat 180 gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa 240 ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca 300 aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat 360 gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg 420
[0155] cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctxcct actccagccg 480 gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta 540 agcgaactgt tcgccagcct gttgaata.tg cagggtgaag aaaaatcccg cgcgctggcg 600 attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt 660 tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa 720 ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc 780 gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa 840 tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag 900 ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat 960 tltgccttct cgctgcatga ccttagtgat aaagaaacca. ccattagcca gcagagtgcc 1020 gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag 1080 cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc 1140 cacggccgtt tagcgcgtgt ttacaacaag ctgtaa; 1176 <210> 3 <211> 297 <212) Sequence of T-e9 terminator <400〉 tgtgttttac tcctcatatt aacttcggtc attagaggcc acgatttgac acatttttac 60 tcaaaacaaa atgtttgcat atctcttata atttcaaatt caacacacaa caaataagag 120 aaaaaacaaa. taatattaat ttgagaatga acaaaaggac catatcattc attaactctt 180 ctccatccat ttccatttca cagttcgata gcgaaaaccg aataaaaaac acagtaaatt 240 acaagcacaa caaatggtac aagaaaaaca gttttcccaa tgccaiaata ctcgaac;297 <210〉4 <211> 1026 <212> Sequence of HPT gene <400> atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 60 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 120 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat丄80
[0156] cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 240 ggggagttta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 300 caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat 360 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 420 atcggtcaat acactacatg gcgtgatttc atatgcgcga tt
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