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一種雙特異性抗體egfr×cd3的構建及應用_4

文檔序號:8453516閱讀:來源:國知局
gcg ggcaccaaactggaactgaaacgccgtacggtggctgcaccatctgtcttcatcttcccgccatctgatgagcagtt g過過過tctgg過過CtgCCtctgttgtgtgCCtgCtg33t33Cttct3tCCC3g3g3ggCC333gt3C3gtgg33ggtgg 過t過過cgccctcc過過tcgggt過過ctccc過gg過g過gtgtc過c過g過gc過gg過c過gc過過gg過c過gc過cct過c過gcctc過gc 過gc過ccctg過cgctg過gc過過過gc過g過ct過eg過g過過過c過c過過過gtct過cgcctgcg過過gtc過ccc過tc過gggcctg過g ctcgcccgtc過c過過過g過gette過過c過gggg過g過gtgtt過g
[0077] 單價單元重鏈氨基酸序列(Vectibix,序列號5)
[0078] QV化犯SGPGLVKPSETLSLTCTVSGGSVSSGDYYWTWIRQSPGKGLEWIGHIYYSGNTNYNPSLKS化 TISIDTSKTQ FSLKLSSVTAADTAIYYCVRDRVTGA抑IWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALG CLV邸YFPEPVTVSWNSGALTSGV肌FPAVLQSSGLY化SSVVTVPSS化GTQTYICNVNHKPSNTKVDKKVEPKSC DKT肌CPPCPAP化LGGPSVFLFPPKP邸TLMISRTPEVTCVVVDVS肥DPEVKFNWYVDGVEVHNAKTKP肥EQYN STYRVVS化 SDIAVEWESNGQPENNYDTTPPVLDSDGSFFLYSDLTVDKSRWQQGNVFSCSVM肥ALHN肌TQK化化SPGK
[0079] 單價單元重鏈棱酸序列(Vectibix,序列號6)
[0080] caggtgcagctgcaggagtcgggcccaggactggtgaagccttcggagaccctgtccctcacctgcact 咨tetet咨咨t咨咨ctcc咨tea咨ca咨t咨咨t咨attactact咨咨acet咨咨atcc咨咨ca咨tcccca咨咨咨aa咨咨咨act咨咨a咨t咨 gattggacacatctattacagtgggaacaccaattataacccctccctcaagagtcgactcaccatatcaattgaca cgtccaagactcagttctccctgaagctgagttctgtgaccgctgcggacacggccatttattactgtgtgcgagat cgagtgactggtgcttttgatatctggggccaagggacaatggtcaccgtctcttcagcgtcgaccaagggcccatc ggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactact tccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacag tcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaa cgtg過過tc過c過過gccc過gc過過c過cc過過ggtgg過c過過g過過過gttg過gccc過過過tcttgtg過c過過過過etc過c過c過tgee caccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatg過tctcccgg過cccctg過ggtc過c過tgcgtggtggtgg過cgtg過gee過eg過過g過ccctg過ggtc過過gttc過過ctggt過 cgtgg過cggcgtgg過ggtgc過t過過tgee過過g過c過過過geegeggg過gg過gc過gt過c過過c過gc過cgt過ccgtgtggtc過 gCgtCCtC3CCgtCCtgC3CC3gg3CtggCtg33tggC33gg3gt3C33gtgC33ggtctCC33C333gCCCtCCC3 gcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtctacaccctgcccccatcccg gg過tg過getg過cc過過g過過cc過ggtc過gcctgtggtgcctggtc過過過ggettet過tccc過geg過c過tcgccgtgg過gt gggagagcaatgggcagccggagaacaactacgataccacgcctcccgtgctggactccgacggctccttcttcctc t過c過geg過tctc過ccgtgg過c過過g過gc過ggtggc過gc過gggg過過cgtcttctc過tgctccgtg過tgc過tg過ggetet gcacaaccactacacgcagaagagcctctccctgtctccgggtaaatga
[0081] 單價單元輕鏈氨基酸序列(Vectibix,序列號7)
[0082] DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGT DFTFTIS化QPEDIATYFCQH抑HLPLAFGGGTKVEIKRTVAAPSVFIFPPSDE化KSGTASVVCLLNNFYP肥AKV QWKVDNALQSGNS犯SVTEQDS邸STTSLSSTLTLSKADYEK服VYACEVTHQGLSSPVTKSFNRGEC
[0083] 單價單元輕鏈棱酸序列(Vectibix,序列號8)
[0084] gacatccagatgacccagtctccatcctccctgtctgcatctgtaggagacagagtcaccatcacttgc c過ggcg過gtc過gg過c過tc過gc過過ct過ttt過過過ttggt過tc過gc過g過過過cc過ggg過過過gcccct過過過ctcctg過tet過eg過tgc過tee過過tttgg過過過c過ggggtccc過tc過過ggttc過gtgg過過gtgg過tctggg過c過g過tttt過ettte過cc過 tcagcagcctgcagcctgaagatattgcaacatatttctgtcaacactttgatcatctcccgctcgctttcggcgga ggg過cc過過ggtgg過g過tc過過過cgt過cggtggctgc過cc過tctgtcttc過tcttcccgcc過tetg過tg過gc過gttg過過 過tctgg過過ctgcctctgttgtgtgcctgctg過過t過過ettet過tccc過g過g過ggee過過過gt過c過gtgg過過ggtgg過t過 過cgccctcc過過tcgggt過過ctccc過gg過g過gtgtc過c過g過gc過gg過c過gc過過gg過c過gc過cct過c過gcctc過gc過gc過ccctg過cgctg過gc過過過gc過g過ct過eg過g過過過c過c過過過gtct過cgcctgcg過過gtc過ccc過tc過gggcctg過gctc gCCCgtC3C333g3gCttC33C3gggg3g3gtgtt3g
[0085] 單鏈單元氨基酸序列(L2K,序列號9)
[0086] DIKLQQSGAELARPGASVKMSCKTSGYTFTRYTMHWVKQRPGQGLEWIGYINPSRGYTNYNQKFKDKAT LTTDKSSSTAYMQLS化TS邸SAVYYCARYY孤HY化DYWGQGTTLTVSSGGGGSGGGGSGGGGSDIQLTQSPAIMS ASPGEKVTMTCRASSSVSYMNWYQQKSGTSPKRWIYDTSKVASGVPYRFSGSGSGTSYSLTISSMEAEDAATYYCQQ WSSNPLTFGAGTKLELKGAAAEPKSCDKTHTCPPCPA陽LLGGPSV化FPPKP邸TLMISRTPEVTCVVVDVS肥DP EVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS化TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQV YTLPPS畑化TKNQV化TCRVKGFYPSDIAVEWESNGQPENNYKTTPPVLKSDGSF化ASKLTVDKSRWQQGNVFSC SVM肥ALHNHYTQK化化SPGK
[0087] 單鏈單元核酸序列(L2K,序列號10)
[0088] GACATCAAACTGCAGCAGTCAGGGGCTGAACTGGCAAGACCTGGGGCCTCAGTGAAGATGTCCTGCAAG ACTTCTGGCTACACCTTTACTAGGTACACGATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGG ATACATTAATCCTAGCCGTGGTTATACTAATTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTACAGACAAAT CCTCC AGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAGATATTA TGATGATCATTACTGCCTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAGGAGGCGGCGGTTCAGGCG GAGGTGGAAGTGGTGGAGGAGGTTCTGACATTCAGCTGACCCAGTCTCCAGCAATCATGTCTGCATCTCCAGGGGAG AAGGTCACCATGACCTGCAGAGCCAGTTCAAGTGTAAGTTACATGAACTGGTACCAGCAGAAGTCAGGCACCTCCCC CAAAAGATGGATTTATGACACATCCAAAGTGGCTTCTGGAGTCCCTTATCGCTTCAGTGGCAGTGGGTCTGGGACCT CATACTCTCTCACAATCAGCAGCATGGAGGCTGAAGATGCTGCCACTTATTACTGCCAACAGTGGAGTAGTAACCCG CTCACGTTCGGTGCTGGGA
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